A method for separating coenzyme q10 using a supercritical fluid chromatography system
A supercritical fluid and chromatographic system technology, applied in the field of material separation and purification, can solve the problems of low purity and yield of coenzyme Q10 crude extract, large amount of solvent, and long time Emissions and the effect of reducing the amount of circulation
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[0039] According to a specific embodiment of the invention, the method of separating the Coenzyme Q10 using supercritical fluid chromatography systems comprises:
[0040] (1) Dissolve the coenzyme Q10 crude tape in the organic solvent to be separated from the coenzyme Q10 feed liquid; open the supercritical fluid chromatography system, set system parameters;
[0041] (2) Stable operation of the system, the column is sufficiently balanced, and the baseline is stable, and the coenzyme Q10 feed liquid to be separated is to collect the coenzyme Q10 component according to the peak signal according to the peak, and according to this separation process, repeat multiple injections ;
[0042] (3) The above-mentioned steps accumulate the accumulated co-enzyme Q10 component to remove the organic solvent and entrained agents in the rotary evaporator to obtain a high purity Co10.
[0043] The invention will be described in detail below with reference to the examples.
[0044] In the following ...
Embodiment 1
[0046] This embodiment uses the column specification of 10 × 250 mm, fixed phase C18, and the particle size of 25 μm, and the supercritical fluid chromatography system separates the Co10 includes the following steps:
[0047] (1) dissolve the coenzyme Q10 crude tissue in methanol to form a coenzyme Q10 feed liquid, concentration of 0.5 mg · ml -1 The purity is 45%; open the supercritical fluid chromatographic system, set the column temperature of the column of the column of 30 ° C, the column pressure is 7 MPa, and the column pressure is a constant pressure, the flow phase flow is 6 cV / min, the flow phase is supercritical carbon dioxide Composition with the jacket, the entrained agent is ethanol and the proportion of the entrained agent in the flow phase is 5% V / V, and the ultraviolet wavelength is 220 nm;
[0048] (2) Stable operation of the system, the column is sufficiently balanced, and the baseline is stable, and the injecting is 15% of the 20% co-enzyme Q10 feed fluid of...
Embodiment 2
[0051] In this example, the chromatographic column is 15 × 250 mm, and the fixed phase surface is coated with three (3,5-dimethylphenylanimoylated) linear silica gel, the particle size is 45 μm, and the supercritical fluid chromatographic system separates The method of Q10 includes the following steps:
[0052] (1) dissolve the coenzyme Q10 crude tape in ethanol and the coenzyme Q10 feed liquid, concentration is 5 mg · ml -1 The purity is 52%; open the supercritical fluid chromatographic system, set the column temperature of its column to 35 ° C, column pressure is 8.5 MPa, and the column pressure is constant pressure, the flow phase flow is 5 cV / min, the flow corresponds to the supercritical Composition of carbon dioxide and jacket, entrained agent is isopropyl alcohol and the proportion of entrained agent is 10% v / v in the flow phase, and the ultraviolet wavelength is 245 nm;
[0053] (2) Stable operation of the system, the column is sufficiently balanced, the baseline is st...
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