A functional polymer material with specific recognition and rapid detection of chromium ions and its preparation and application
A technology of functional polymers and chromium ions, applied in the field of detection, can solve the problems of inapplicable rapid detection of heavy metal ions, complex and cumbersome operation process, and high technical requirements for detection personnel, and achieve the effects of low cost, good selectivity and high sensitivity
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Embodiment 1
[0041] (1) Preparation of aldylated chitosan: get the chitosan of 10g molecular weight 50000 and place it in the reaction vessel of 65-68% nitric acid, 85% phosphoric acid mixed acid, wherein the addition of mixed acid is 140ml , the volume ratio of nitric acid and phosphoric acid is 4:1, and sodium nitrite is subsequently added, and the mass ratio of the added amount to the amount of chitosan is 1:5. Seal the reaction for 8 hours, add 500ml of deionized water to the reaction vessel after the reaction, and air-dry the prepared aldehylated chitosan at 50°C for 24 hours for use.
[0042] (2) Introducing carboxyl functional groups. Get 1g of the prepared aldehylation chitosan and place it in the reaction container, add 20ml PBS buffer solution in advance in this container, the pH of this buffer solution is 7.2, add glutamic acid, the addition amount is the same as the formaldehyde chitosan The mass ratio of sugar was 1:1. The reaction was carried out at 30°C and 3000rpm magnetic...
Embodiment 2
[0045] (1) Preparation of aldylated chitosan: get the chitosan of 10g molecular weight 50000 and place it in the reaction vessel of 65-68% nitric acid, 85% phosphoric acid mixed acid, wherein the addition of mixed acid is 150ml , the volume ratio of nitric acid and phosphoric acid is 5:1, followed by adding sodium nitrite, the mass ratio of the amount added to the amount of chitosan is 1:4. Seal the reaction for 8 hours, add 500 ml of deionized water to the reaction vessel after the reaction, and place the prepared aldehylated chitosan in a blast drying oven at 50° C. for 24 hours before use.
[0046] (2) Introducing carboxyl functional groups. Get 1g of prepared aldehylation chitosan and place it in a reaction container, add 20ml of PBS buffer solution in advance in this container, the pH of this buffer solution is 7.4, add glutamic acid, and the addition amount is the same as that of the aldehylation shell The mass ratio of polysaccharides was 1:2, 30°C, 3000rpm magnetic st...
Embodiment 3
[0049] (1) prepare aldylated chitosan: get 10g molecular weight and be the chitosan of 50000 and place it in the reaction vessel of 65-68% nitric acid, 85% phosphoric acid mixed acid, wherein mixed acid consumption is 140ml, The volume ratio of nitric acid and phosphoric acid is 4:1, followed by adding potassium nitrite, and the mass ratio of the added amount to the amount of chitosan is 1:5. Seal the reaction for 8 hours, add 500 ml of deionized water to the reaction vessel after the reaction, and place the prepared aldehylated chitosan in a blast drying oven at 50° C. for 24 hours before use.
[0050] (2) Introducing carboxyl functional groups. Get 1g of the prepared aldylated chitosan and place it in a reaction container, add 20ml of PBS buffer in advance in this container, the pH of this buffer is 7.2, add 2-aminoethanesulfonic acid, and the addition amount is the same as aldehyde The mass ratio of ylated chitosan is 1:1, 30°C, 3000rpm magnetic stirring reaction for 4 hou...
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