Application of kelp extract
A kind of kelp extract and extract technology, which is applied in the application field of kelp extract, can solve problems such as dryness, tightness and cracking, and decreased skin elasticity, so as to improve stability, adjust skin moisturizing ability, and adjust skin resistance. The effect of oxidative capacity
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Embodiment 1
[0051] Embodiment 1, the preparation of kelp extract
[0052] Freeze the fresh kombu at -40°C, take it out after freezing for 12 days, and thaw it in air at room temperature until the central temperature of the kombu reaches 0°C; put the kombu in a centrifuge and centrifuge at a speed of 5000rpm 7min to remove water; through repeated freeze-thaw and centrifugation 3 times, control the water content of kelp to 70%. Weigh 100g kelp and put it into a 1000mL conical beaker, add 500mL of 85% (V / V) ethanol aqueous solution, seal the mouth of the conical beaker with plastic wrap, leaching for 1 hour in the dark, and filter. Continue to add 500 mL of 85% (V / V) ethanol aqueous solution to the above-mentioned leaching treatment of kelp, seal the mouth of the triangular beaker with plastic wrap, and leaching for 1 hour in the dark, filter, and combine the filtrates. Concentrate the filtrate at 40°C. During the concentration, when a large amount of precipitation gradually appears in the so...
Embodiment 2
[0053] Embodiment 2, detection of free radical scavenging activity of kelp extract DPPH method
[0054] 1,1-Diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl, DPPH) is a stable nitrogen-centered organic radical, and its ethanol solution is dark purple at about 517nm There is strong absorption; when there is a free radical scavenger, the absorption of DPPH is weakened and gradually disappears due to its single-electron pairing. The decolorization degree of DPPH ethanol solution has a quantitative relationship with the number of electrons it accepts, so a spectrophotometer can be used for rapid quantitative analysis to detect free radical scavenging and evaluate the ability of antioxidants to scavenge free radicals.
[0055] Preparation of DPPH ethanol solution: Accurately weigh 6mg of DPPH, dissolve it with 95% ethanol and set the volume in a 50mL volumetric flask, and dissolve with the aid of ultrasound. When used, dilute it four times with 95% ethanol to obta...
Embodiment 3
[0067] Embodiment 3, detection of kelp extract ABTS scavenging ability
[0068] Dissolve ABTS (2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt) (+H 2 O) until the final concentration is 7mmol / L, potassium persulfate is formulated into 2.45mmol / L, the two solutions are mixed 1:1, placed in the dark, and stirred at room temperature for 16h; then the ABTS mixed solution is mixed with PBS solution (PH= 7.0) Dilute until its absorbance value (measured at 734nm) is 0.70±0.02; the kelp extract is diluted to different concentrations with petroleum ether / ethanol (1:1), respectively 1.5μg / mL, 3μg / mL, 6μg / mL , 13μg / mL, 25μg / mL, 50μg / mL, 100μg / mL; finally, add the above-mentioned samples of different concentrations into the ABTS mixed solution, the ratio of the sample to the ABTS mixed solution is 1:20, mix and incubate at room temperature for 6min , The absorbance was measured at 734nm.
[0069] Calculation formula: ABTS clearance rate (%) = (1-A1 / A0) × 100%
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