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Collagen peptide for controlling osteoarthritis of old people and preparation method of collagen peptide for controlling osteoarthritis of old people

A collagen peptide and osteoporosis technology, applied in the field of collagen peptide preparation, can solve the problems of low molecular weight, high salt content, and no biological activity, so as to improve and inhibit the decline of bone density, promote cell proliferation, and improve The effect of purity

Inactive Publication Date: 2020-08-25
WUXI KESHANG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the prepared collagen peptides have the following problems: low molecular weight, mostly below 1000 Da, mostly in the form of free amino acids or oligopeptides; low purity, high salt content; no biological activity, only As a nutritional supplement of amino acids; fewer types of amino acids, lower levels of glycine, hydroxyproline / proline, and glutamic acid

Method used

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  • Collagen peptide for controlling osteoarthritis of old people and preparation method of collagen peptide for controlling osteoarthritis of old people
  • Collagen peptide for controlling osteoarthritis of old people and preparation method of collagen peptide for controlling osteoarthritis of old people
  • Collagen peptide for controlling osteoarthritis of old people and preparation method of collagen peptide for controlling osteoarthritis of old people

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A collagen peptide for preventing and treating osteoporosis in the elderly, the preparation method of the collagen peptide comprises the following steps:

[0033] (1) Use a shaver to extract the long hair on the surface of the wet cowhide, scrape off the fat layer as much as possible with a scraper, clean it, cut it into pieces, and freeze it for later use.

[0034] (2) Soak 100 g of the pretreated cowhide with 1000 mL of NaOH aqueous solution (0.5% in mass concentration) for 1 hour. After soaking, wash the cowhide with purified water until neutral.

[0035] (3) Soak the cowhide in purified water (solid-liquid ratio 1:5g / mL), place in an autoclave, and heat-treat under pressure at 115°C and 0.05MPa for 3h.

[0036] (4) Then use a filter bag to filter hair and impurities while it is hot, refrigerate at 4°C for 12 hours, and filter fat with gauze to obtain kraft gum.

[0037] (5) Adjust the pH to 6.0±0.2 with 2M HCl solution, add 1% (based on the dry product of cowhide g...

Embodiment 2

[0040] A collagen peptide for preventing and treating osteoporosis in the elderly, the preparation method of the collagen peptide comprises the following steps:

[0041] (1) Use a shaver to extract the long hair on the surface of the wet pigskin, scrape off the fat layer as much as possible with a scraper, clean it, cut it into pieces, and freeze it for later use.

[0042] (2) Soak 100 g of pretreated pigskin with 800 mL of NaOH aqueous solution (mass concentration: 0.6%) for 1.2 hours. After soaking, wash the pigskin with purified water until neutral.

[0043] (3) Soak pigskin in purified water (solid-liquid ratio 1:6g / mL), place in an autoclave, and heat-treat under pressure at 118°C and 0.1MPa for 2.5h.

[0044] (4) Then use a filter cloth bag to filter hair and impurities while it is hot, refrigerate at 6°C for 18 hours, and filter fat with gauze to obtain pigskin gelatin.

[0045] (5) Adjust the pH of the pigskin jelly to 7.0±0.2 with 2M HCl solution, add 0.5% (based on ...

Embodiment 3

[0048] A collagen peptide for preventing and treating osteoporosis in the elderly, the preparation method of the collagen peptide comprises the following steps:

[0049] (1) Clean the wet fish skin, cut it into pieces, and freeze it for later use.

[0050] (2) Soak 100 g of pretreated fish skin with 500 mL of NaOH aqueous solution (mass concentration 0.5%) for 0.5 h. After soaking, wash the fish skin with purified water until neutral.

[0051] (3) Soak the fish skin in purified water (solid-liquid ratio 1:10g / mL), place it in an autoclave, and heat-treat it under pressure at 120°C and 0.05MPa for 2h.

[0052] (4) Then filter out impurities with a filter bag while it is hot, refrigerate at 8°C for 24 hours, filter out fat with gauze, and obtain fish skin gelatin.

[0053] (5) Adjust the pH of the fish skin gel to 6.5±0.2 with 2M HCl solution, add 0.5% (based on the dry product of the fish skin gel) of papain and 1% of Bacillus licheniformis neutral protease, 40°C The enzymati...

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Abstract

The invention discloses collagen peptide for controlling osteoarthritis of old people and a preparation method of the collagen peptide for controlling osteoarthritis of old people, and belongs to thetechnical field of preparation of the collagen peptide. The preparation method of the collagen peptide comprises the following steps of performing pretreatment, performing alkali treatment, performingpressurized heat treatment, performing filtering, removing hair and degreasing, performing enzymatic hydrolysis and enzyme deactivation, performing decoloring, deodorization and filtration, performing spray drying and the like. An alkali treatment method is adopted, so that the isoelectric point of the collagen peptide is 5 or below, and the degree of amidation is 5% or below. By an alkaline treatment-enzymatic hydrolysis method, the macrochain can be effectively sheared, amino acid groups and polypeptide chains can be completely reserved, the content of the collagen peptide of which the molecular weight is 3000-6000Da is 75% or above, the obtained polypeptide has biological activity, and the purity of the collagen peptide can achieve 95% or above.

Description

technical field [0001] The invention relates to a collagen peptide for preventing and treating osteoporosis in the elderly and a preparation method thereof, belonging to the technical field of collagen peptide preparation. Background technique [0002] The synthesis of collagen is the most vigorous around the age of 25. With the increase of age, the amount of collagen synthesis decreases year by year. At the age of 60, the content of collagen is only 50% of that of youth. The reduction of collagen causes the body's connective tissue The resistance of the body is reduced, and the metabolism of bone collagen is abnormal, which leads to chronic orthopedic diseases, such as arthralgia, arthritis, rheumatoid arthritis and osteoporosis. [0003] Collagen peptides can alleviate the symptoms of osteoarthritis (OA), and can repair bone and joint damage or slow down degeneration without side effects. Collagen peptide can stimulate articular cartilage to produce type II collagen, hyal...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06C07K1/34A61P19/10
CPCA61P19/10C07K14/78C12P21/06
Inventor 陆金婷许亚丽石丽虹程咏梅任伟业
Owner WUXI KESHANG BIOLOGICAL TECH
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