Preparation method of tremella polysaccharide

A technology of tremella polysaccharide and molecular weight, applied in the field of preparation of tremella polysaccharide, can solve the problem of difficult control of raw materials and process parameters, no controlled degradation to prepare high-quality tremella polysaccharide products, lack of raw materials and product purity, molecular weight distribution range monitoring, etc. question

Inactive Publication Date: 2020-09-11
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the prior art only prepares low-molecular-weight tremella polysaccharides in the extraction process. Although the production cost is relatively low, there are problems such as difficult control of raw materials and process parameters, and uneven molecular weight distribution of the final product; Lack of key quality control methods such as raw material and product purity, molecular weight distribution range monitoring
In the existing disclosed technical solutions, there is no bibliographical report on the preparation of high-quality tremella polysaccharide products by controlled degradation

Method used

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  • Preparation method of tremella polysaccharide
  • Preparation method of tremella polysaccharide
  • Preparation method of tremella polysaccharide

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preparation example Construction

[0026] The purpose of the present invention is to provide a preparation method of tremella polysaccharide, which selects tremella polysaccharide with a molecular weight greater than 1500kDa for controlled acid hydrolysis, so that tremella polysaccharide with a specific molecular weight in the range of 10-1000kDa can be prepared. And high performance size exclusion chromatography (HPSEC) was used to measure the molecular weight of tremella polysaccharide, and the ultraviolet color method was used to determine the content. Specifically include the following steps:

[0027] (1) select a homogeneous tremella polysaccharide raw material with a molecular weight greater than 1500kDa, and prepare a 0.5%-1.5% tremella polysaccharide solution;

[0028] In this step, the tremella polysaccharide can be extracted from the fruit body, sporophyte or submerged fermentation broth of Tremella fungus, and the extraction method and raw material of the tremella polysaccharide can be obtained from ...

Embodiment 1

[0040] Preparation of tremella polysaccharide by controlled acid hydrolysis.

[0041] A preparation method of tremella polysaccharide, comprising the steps of:

[0042] (1) Select 10.0 g of tremella polysaccharide (TFP) raw material with a molecular weight greater than 1500kDa, and prepare a 1.0% (W / V, the same below) tremella polysaccharide solution with purified water, wherein tremella polysaccharide (TFP, batch number WSK181117) is white Solid powder, provided by Shanghai Huiwen Biotechnology Co., Ltd. The main extraction process is as follows: take the raw material of tremella fruiting body, extract with hot water, concentrate, decolorize, ethanol precipitation, filter, dry, grind to obtain dried tremella Entity polysaccharide powder. G6000 chromatographic column was used for HPSEC analysis, and the relative molecular weight of TFP was determined to be 3843kD based on dextran;

[0043] (2) Add 2 mol / L HCl to the tremella polysaccharide solution and adjust it to an acidit...

Embodiment 2

[0049] Detection and Analysis of Tremella Polysaccharide

[0050] In order to determine the purity and quality of the tremella polysaccharide prepared in Example 1 and carry out quality control, the present invention has established the molecular weight and content assay method of the tremella polysaccharide:

[0051] (1) Determination of average weight average molecular weight

[0052] The HPSEC method was used to determine the molecular weight with a series of molecular weight dextran as the standard. Through the exploration of mobile phase, temperature and flow rate, it was found that in (NH 4 ) 2 SO 4 Polysaccharide samples in saline had less baseline fluctuation, better peak shape, and higher resolution. Determine the optimized liquid phase conditions: TSK-gel G4000

[0053] PWXL chromatographic column (7.8x 300mm); mobile phase 0.1mol L-1 (NH 4 ) 2 SO 4 , flow rate 0.5mL·min -1 ; Differential refractive index detector; column temperature 30°C; injection volume 2...

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Abstract

The invention discloses a preparation method of tremella polysaccharide. A uniform tremella polysaccharide raw material with the molecular weight larger than 1500 kD is adopted, wherein hydrolysis isconducted for 0.5-10 hours within the acidity range of 0.05-0.15 mol / L and the temperature range of 70-100 DEG C, and tremella polysaccharide with the specific molecular weight within the range of 10-1000 kDa is obtained. The molecular weight of the tremella polysaccharide acid hydrolysate is detected by adopting a high-performance molecular exclusion chromatography, and the content of the tremella polysaccharide acid hydrolysate is determined by adopting an ultraviolet developing method; the prepared tremella polysaccharide is an acidic heteropolysaccharide taking alpha-(1->3)-D-mannose as amain chain, and the tremella polysaccharide acid hydrolysate completely reserves a structural unit of prototype tremella polysaccharide. The method can be used for obtaining tremella polysaccharide hydrolysate with controllable molecular weight and good uniformity, has the advantages of simplicity and convenience in operation, easiness in control, suitability for industrial production and the like, and can be applied to the fields of foods, cosmetics, medicines and the like.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of white fungus polysaccharide. Background technique [0002] Tremella fueiforms Berk, also known as white fungus, white fungus, white fungus, etc., is a traditional Chinese medicinal material for both medicine and food. Aging, regulating blood sugar and blood lipids, protecting gastric mucosa, and anti-radiation. Tremella polysaccharide is the main functional component of Tremella, which can be extracted from its fruiting body, sporophyte or submerged fermentation broth. Tremella polysaccharide has a complex structure, mainly a type of heteropolysaccharide with α-(1-3)-D-mannose as the main chain, and contains complex branched chains composed of xylose, glucuronic acid and other monosaccharides. Tremella polysaccharides prepared from different raw material sources and processing techniques are different in molecular weight, monosac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 刘江云单佳晶李雅丽李冉
Owner SUZHOU UNIV
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