Glucose sensor

A technology of glucose sensor and glucose dehydrogenase, which is applied in the field of glucose dehydrogenase composition, can solve the problems of small sensor response current blank value, inability to fully improve storage stability, inability to obtain and the like

Inactive Publication Date: 2003-07-30
PANASONIC HEALTHCARE HLDG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] However, when PQQ-GDH enzyme is used, even if the above-mentioned additives such as phosphoric acid are added to the reaction layer, a sufficiently small sensor response current blank value cannot be obtained, and there is a problem that the storage stability cannot be sufficiently improved.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0065] exist figure 1 0.5 wt% aqueous solution of carboxymethylcellulose sodium salt (hereinafter referred to as "CMC") as a hydrophilic polymer was dropped on the electrode of the substrate 1, and dried in a warm air dryer at 50°C for 10 minutes to form CMC. Layer 7. Then, a mixed solution of 5000 units of PQQ-GDH, 20 μM potassium hydrogen phthalate and 50 μM potassium ferricyanide dissolved in 1 ml of water was dropped on the CMC layer 7, and the reaction layer 8 was formed after drying. In this way, a glucose sensor was fabricated.

[0066] Then, aqueous glucose solutions of various concentrations were prepared as sample solutions, and the above sample solutions were dropped on the reaction layer 8 . Once the sample solution containing glucose is dropped on the reaction layer, the glucose in the sample is oxidized by PQQ-GDH. At the same time, potassium ferricyanide in the reaction layer is reduced to potassium ferrocyanide.

[0067] After adding the sample solution dro...

Embodiment 2

[0080] Except that the CMC layer 7 was not formed on the electrodes, a glucose sensor was manufactured in the same manner as in Example 1. Then, in the same manner as in Example 1, graphs were drawn of the response characteristics of the sensor immediately after production and after storage for 6 months.

[0081] As a result, there is a certain correlation between the glucose concentration and the current value, which has a good linear relationship. The blank value was also small, and there was almost no difference in response characteristics between the sensor immediately after production and the sensor stored for 6 months, showing good storage stability.

Embodiment 3～10

[0083]In addition to using maleic acid (Example 3), succinic acid (Example 4), triethanolamine hydrochloride (Example 5), sodium dihydrogen citrate (Example 6), dimethylglutaric acid (Example 7), 2-(N-morpholino) ethanesulfonic acid (embodiment 8), three (hydroxymethyl) glycine (embodiment 9) or tris (hydroxymethyl) aminomethane (embodiment 10) replace benzene Except for potassium hydrogen diformate, the other operations were the same as in Example 1 to prepare a glucose sensor, and then draw the response characteristic diagram of the sensor just after fabrication and after storage for 6 months. The response characteristic diagram is as follows Figure 4 ~ Figure 11 shown.

[0084] from Figure 4 ~ Figure 11 It can be seen that the blank value of the fabricated sensor is very small, and there is a certain correlation between the concentration and the current value. Furthermore, the responsiveness was high and a good linear relationship was shown. In addition, there was alm...

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Abstract

The present invention provides a high performance glucose sensor which can demonstrate high stability against preservation and produces only a low blank value. The glucose sensor comprises an electrically insulating base plate, an electrode system formed on the base plate, and a reaction layer which is formed in contact with or in the vicinity of the electrode system and contains at least a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the reaction layer further containing an additive such as phthalic acid.

Description

technical field [0001] The present invention relates to a glucose sensor capable of quickly and easily quantifying a specific component in a sample with high precision. More specifically, the present invention relates to a method for stabilizing glucose dehydrogenase used in a glucose sensor using pyrroloquinoline quinone as a coenzyme and a stable composition of glucose dehydrogenase. Background technique [0002] Conventionally, various biosensors have been proposed as means for simply quantifying a specific component in a sample solution without diluting or stirring the sample solution. The following is an example of a biosensor (Japanese Patent Laid-Open Publication No. Hei 2-062952). [0003] The biosensor includes an electrode system composed of a working electrode, a counter electrode, and a reference electrode formed on an insulating substrate by screen printing and other methods, and an electrode system composed of a hydrophilic polymer, an oxidoreductase, and a E...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327C12Q1/00G01N27/416G01N33/487
CPCC12Q1/006G01N27/3271
Inventor 汤川系子吉冈俊彦南海史朗岩田润子宫崎正次马场英行竹嶋诚嗣
Owner PANASONIC HEALTHCARE HLDG CO LTD
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