Styrene cyclooxygenase derived from rhizobium, and function of styrene cyclooxygenase

A cyclooxygenase, styrene technology, applied in the direction of oxidoreductase, enzymes, microorganism-based methods, etc., to achieve the effect of excellent enantioselectivity
CN111662887AActive Publication Date: 2020-09-15CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Publication Date
2020-09-15

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Abstract

The invention discloses a novel styrene mono-cyclooxygenase BrSMO derived from Bradyrhizobium sp. ORS 375, and an application of the novel styrene mono-cyclooxygenase BrSMO in the catalysis of asymmetric oxidation reactions. The enzyme can catalyze styrene or thioether substrates to carry out asymmetric oxidation reactions, and has relatively high activity, wherein the styrene substrates are asymmetrically oxidized to generate S-type epoxy, the enantioselectivity is excellent, and the ee is greater than 99%; and the thioether substrates are asymmetrically oxidized to generate R-type sulfoxide,and the ee value of generated (R)-p-bromophenyl methyl sulfoxide is up to 90%.
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Description

technical field

[0001] The invention relates to a novel styrene cyclooxygenase BrSMO gene and its catalytic function from Bradyrhizobium sp. engineering field. Background technique

[0002] Chirally pure epoxides are important synthetic building blocks for the production of pharmaceuticals and fine chemicals, yet obtaining epoxides with high yields and excellent optical purity remains a great challenge in organic synthesis. To date, many chemical and enzymatic strategies have been developed to synthesize chiral epoxides. Among them, the biocatalytic epoxidation of alkenes is considered to be a green and efficient route to prepare chiral pure epoxy compounds.

[0003] Styrene cyclooxygenase, which can catalyze the epoxidation of alkenes to obtain epoxides with excellent enantioselectivity, is an excellent biocatalyst for the preparation of chiral epoxides. At present, many styrene cyclooxygenase genes have been identified from styrene-degrading strains, metagenomes, or thr...

Claims

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