Kit for anxiolytic and hypnotic drug in serum by ultra-high performance liquid chromatography-tandem mass spectrometry technology

Abstract

The invention relates to a kit for detecting an anxiolytic and hypnotic drug in serum by an ultra-high performance liquid chromatography-tandem mass spectrometry technology. 11 kinds of anxiolytic andhypnotic drugs can be detected at one time; target drugs and metabolites are monitored at the same time; the pretreatment process is simple, low in cost, high in sensitivity and high in specificity,separation and detection of the anxiolytic and hypnotic drugs are completed within 5 minutes, the accuracy and precision basically meet the requirements, the kit can be used for quantitative analysisof the anxiolytic and hypnotic drugs clinically, and a simple and rapid detection method is provided for monitoring the treatment concentration of the anxiolytic and hypnotic drugs in serum clinically.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a kit for detecting anti-anxiety and hypnotic drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. The specific drugs are: Nitrazepam (NZP), Oxazepam ( Oxazepam (OXP), Estazolam (ESL), Temazepam (Temazepam, TMP), Alprazolam (Alprazolam, APL), Bromazepam (BZP), Lorazepam (LZP) ), midazolam (Midazolam, MDZ), zopiclone (Zopiclone, ZPC), diazepam (Diazapam, DZP) and zolpidem (Zolpidem, ZPM). Background technique [0002] Anxiety and insomnia is a common disease, which is generally affected by biological, psychological and social aspects. Benzodiazepines belong to a kind of psychotropic drugs, which have the effects of sedative, hypnotic, anxiolytic, anticonvulsant, and muscle relaxation. Benzodiazepines are drug-related benzodiazepine receptor agonists that exert a depressant effect on the central nervous system (CNS) and are ...

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Problems solved by technology

[0009] At present, there are many patents for the detection of the concentration of anti-anxiety and hypnotic drugs in the body. For example, the Chinese patent application (publication number: CN 109085265 A) discloses a "liquid chromatography Kit and Application of Tandem Mass Spectrometry for Detecting Anxiolytic / Hypnotic Drugs in Serum and Plasma", 8 kinds of substances are detected at the same time, the sample volume needs to be 100 μL, and the pretreatment takes more than 35 minutes, Chinese patent application (publication number: CN 107991421 A) published "A liquid chromatographic analysis method for detecting the content of diazepam in blood", the sensitivity of diazepam is low, the limit of quantification is 0.625μg / mL, the sample volume needs to be 100μL, the pretreatment is cumbersome, extraction is required, nitrogen blowing is concentrated

In addition, there are patents for the detection of one or several of these drugs, which have relatively low efficiency, long analysis time for a single sample, low sensitivity, unsuitable linear range, complicated sample pretreatment, and matrix effect interference.

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