A kind of Lactobacillus paracasei lc-37 with promoting digestion and its application
A technology of LC-37 and Lactobacillus, which is applied in the direction of Lactobacillus, cheese substitutes, bacteria used in food preparation, etc., can solve the problems of lack of effective evaluation and lack of promotion of digestion, and achieve improvement of indigestion, abdominal pain, increase The effect of content
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Embodiment 1
[0046] The acquisition of embodiment 1 Lactobacillus paracasei strain LC-37
[0047] 1. Isolation of Lactobacillus paracasei strain LC-37
[0048] The intestinal contents of the healthy and long-lived elderly people in Guangxi Bama were collected, that is, feces, and the intestinal feces samples of the healthy and long-lived elderly people in Guangxi Bama were serially diluted with diluent. Different morphological colonies, microscopically select Gram-positive bacilli, and continue to use the pour plate method to purify the strain until the pure culture of the strain is isolated. After the above separation process, a Lactobacillus strain was obtained.
[0049] The preparation method of the diluent used in the above dilution step: 4.5 g of sodium dihydrogen phosphate, 6.0 g of disodium hydrogen phosphate, 0.5 g of L-cysteine, 0.5 g of agar, 0.5 mL of Tween 80, 1000 mL of distilled water, Heat to dissolve, adjust pH to 7.4-7.6, sterilize at 121°C for 15min, and set aside.
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Embodiment 2
[0053] Embodiment 2 Lactobacillus paracasei LC-37 bacteria powder
[0054] The present embodiment provides a preparation method of Lactobacillus paracasei LC-37 bacterial powder, which specifically includes the following steps:
[0055] 1. Expanded culture of strains
[0056] The Lactobacillus paracasei LC-37 cryopreserved strain was inoculated into 10 mL of MRS medium, and placed at 37 °C for 12 hours to obtain a seed culture solution suitable for inoculation. bacteria.
[0057] 2. Fermentation
[0058] The seed culture liquid obtained above was inoculated into the fermentation medium at an inoculum amount of 2% to 5% (v / v) (2% v / v was selected in this example). In this example, the fermentation medium was: The MRS medium was cultured at 33°C with a stirring speed of 200 rpm, the pH of the medium was kept at pH 5.0, and the fermentation was carried out for 20 hours. After the fermentation, the culture solution was centrifuged by using a disc centrifuge at a rotational spe...
Embodiment 3
[0061] Embodiment 3 Lactobacillus paracasei LC-37 bacterial powder
[0062] The present embodiment provides a preparation method of Lactobacillus paracasei LC-37 bacterial powder, which specifically includes the following steps:
[0063] 1. Expanded culture of strains
[0064] The Lactobacillus paracasei LC-37 cryopreserved strain was inoculated into 10 mL of MRS medium, and placed at 37 °C for 12 hours to obtain a seed culture solution suitable for inoculation. bacteria.
[0065] 2. Fermentation
[0066] The seed culture liquid obtained above was inoculated into the fermentation medium at an inoculum amount of 2% to 5% (v / v) (5% v / v was selected in this example). In this example, the fermentation medium was: The MRS medium was cultured at 40° C., the stirring speed was 100 rpm, the pH of the medium was maintained at pH 6.0, and the fermentation was carried out for 14 hours. After the fermentation, the culture solution was centrifuged with a disc centrifuge at a rotational...
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