Collagen-based cell scaffold with vascular regeneration promoting property and preparation method thereof

A regenerative performance, cell scaffolding technology, applied in medical science, prosthesis, etc., can solve the problems of unfavorable application, low starch solubility, etc., and achieve good biocompatibility, high mechanical strength, and biodegradable promotion effects

Active Publication Date: 2020-09-29
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solubility of starch in water is low, which is not conducive to its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Dissolve starch in deionized water at 80 °C to obtain a starch solution with a mass concentration of 7%, and then add 0.5 parts by volume of hydrogen peroxide solution with a mass concentration of 30% and 1 volume part with a mass concentration of 0.05% to 1 volume part of the starch solution. Copper sulfate solution, then the mixed solution was stirred and reacted at 50 °C for 10 h, after it was cooled to room temperature, it was centrifuged at 3000 rpm for 20 min, absolute ethanol was added to the supernatant, and the filter cake was obtained by suction filtration. After washing the filter cake with water and ethanol for 4 times, the filter cake was dried at 60 °C for 36 h to prepare oxidized starch containing carboxyl groups; the oxidized starch and collagen were dissolved in morpholineethanesulfonic acid solution with a concentration of 30 mM and a pH of 4.5 Obtaining the oxidized starch solution that mass concentration is 0.5% and the collagen solution that mass con...

Embodiment 2

[0017] Dissolve starch in deionized water at 95 °C to obtain a starch solution with a mass concentration of 5%, and then add 1 volume part of a 10% hydrogen peroxide solution and 0.5 volume parts of a mass concentration of 0.1% to 1 volume part of the starch solution. Copper sulfate solution, then the mixed solution was stirred and reacted at 65 °C for 6 h, after it was cooled to room temperature, it was centrifuged at 5000 rpm for 10 min, absolute ethanol was added to the supernatant, and the filter cake was obtained by suction filtration. After washing the filter cake with water and ethanol for 3 times, the filter cake was dried at 40 °C for 48 h to prepare oxidized starch containing carboxyl groups; the oxidized starch and collagen were dissolved in morpholine ethanesulfonic acid solution with a concentration of 35 mM and a pH of 4.7 Obtain the oxidized starch solution that mass concentration is 0.6% and the collagen solution that mass concentration is 0.7%, then 0.12 mass p...

Embodiment 3

[0019] Dissolve starch in deionized water at 100 °C to obtain a starch solution with a mass concentration of 10%, and then add 2 volume parts of hydrogen peroxide solution with a mass concentration of 15% and 0.1 volume parts with a mass concentration of 0.5% to 1 volume part of the starch solution. Copper sulfate solution, then the mixed solution was stirred and reacted at 70 °C for 2 h, after it was cooled to room temperature, it was centrifuged at 4500 rpm for 15 min, absolute ethanol was added to the supernatant, and the filter cake was obtained by suction filtration. After washing the filter cake with water and ethanol for 3 times, the filter cake was dried at 55 °C for 40 h to prepare oxidized starch containing carboxyl groups; the oxidized starch and collagen were dissolved in morpholineethanesulfonic acid solution with a concentration of 50 mM and a pH of 5 Obtaining the oxidized starch solution that mass concentration is 1% and the collagen solution that mass concentra...

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Abstract

The invention relates to a collagen-based cell scaffold with vascular regeneration promoting performance and a preparation method thereof. According to the method, firstly, oxidized starch containingcarboxyl is prepared by employing a wet oxidation method; subsequently, an amidation reaction between the carboxyl of the oxidized starch and amidogen of collagen is realized under the catalytic action of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide; and the collagen-based cell scaffold with a uniform and controllable microscopic porous structure, a good mechanical property and the vascular regeneration promoting property is prepared by regulating the carboxyl content of the oxidized starch. The microenvironment of the collagen-based cell scaffold prepared by the invention can promote adipose-derived stem cells gradually transformed from adherent growth to aggregate growth in the scaffold, thereby inducing the adipose-derived stem cells to regulate high expression of a vascular endothelial growth factor and a fibroblast growth factor, promoting vascular regeneration of a wound, and accelerating healing of a wound surface. The collagen-based cellscaffold provided by the invention has potential market application prospects in the field of biomedical materials.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a collagen-based cell scaffold with controllable microscopic pore structure size, effective promotion of regeneration of blood vessels and adipose tissue, good biocompatibility and biodegradability, and a preparation method thereof. Background technique [0002] Tissue engineering is a new marginal discipline developed in the late 1980s. Tissue engineering provides an effective way for tissue regeneration. Due to the huge demand of soft tissue defects in clinic, the research of tissue engineering in adipose tissue regeneration is promoted. At present, some methods have been used for the regeneration of adipose tissue: scaffold-guided tissue regeneration, injectable systems, block amphiphilic copolymer fragments, etc., but the above methods all focus on the regeneration of fat itself. Studies have shown that the effect of promoting fat regeneration by inducing angiog...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/24A61L27/56A61L27/58
CPCA61L27/20A61L27/24A61L27/56A61L27/58C08L3/10C08L89/00
Inventor 葛黎明解晓芬林先玉李德富穆畅道
Owner SICHUAN UNIV
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