New preparation method and application of kombucha protein

A technology of kombucha and a new method, applied in the field of kombucha protein, can solve the problem of low output content of antibacterial protein, achieve good bacteriostatic effect, mild conditions, and improve the extraction rate.

Pending Publication Date: 2020-10-02
上海澜海生物科技有限公司
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Problems solved by technology

Literature (Xie Junjie, She Shiwang, Zhong Qingping, Yan Xueming, Li Huaguang. Research on Antibacterial Activity of Kombucha Antibacterial Proteins[J]. Biotechnology, 1999(06):22-25.) Antibacterial proteins were extracted from the cultured Kombucha fermentation broth And its an...

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  • New preparation method and application of kombucha protein
  • New preparation method and application of kombucha protein

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Embodiment 1

[0031] A novel method for preparing kombucha protein, comprising the steps of:

[0032] (1) Boil 100g of water, add 0.5g of black tea leaves, decoct for 15 minutes under boiling conditions, filter to remove tea leaves, add 10g of sucrose and 0.1g of MgCl to the tea soup 2 , 1gK 2 HPO 4 Stir to dissolve completely, then cool the tea soup, add 1.5g of Kombucha strain (Kombucha Xinsheng No. Irradiate under the sun for 10s, then ferment for 10 days at a constant temperature of 30°C in an electric thermostat to obtain a kombucha fermentation broth (volume V1);

[0033] (2) dissolving isopropanol in water to form an organic solution with a mass fraction of 26%, dissolving ammonium sulfate in water to form a salt solution with a mass fraction of 12%, and then mixing the organic solution and the salt solution uniformly to form isopropanol Propanol-ammonium sulfate aqueous two-phase liquid;

[0034] (3) Filter the kombucha fermented liquid obtained in step (1), centrifuge the filtr...

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Abstract

The present invention discloses a new preparation method and an application of kombucha protein, and belongs to the technical field of kombucha protein. The method comprises the following steps: adding black tea leaves after water boiling out, conducting decocting for 5 min-1 h under a boiling-out condition, filtering tea leaf residues, adding sucrose and inorganic salt to tea liquid for stirringand complete dissolving, then cooling the tea liquid, adding kombucha in the cooled tea liquid, conducting irradiation under weak ultraviolet lights after uniform stirring, then conducting constant-temperature fermentation in an electric thermostat to obtain kombucha fermentation liquid; and filtering and centrifuging the kombucha fermentation liquid, collecting supernatant, also adding two-aqueous-phase liquid formed by organic reagent-salt, conducting uniform stirring, conducting centrifugation after putting still and sedimentation, collecting supernatant, repeating for multiple times, concentrating the supernatant under reduced pressure to obtain a concentrated solution, and drying the concentrated solution to obtain the kombucha protein. The new preparation method can significantly increase output content of the kombucha protein extracted from the kombucha fermentation liquid.

Description

technical field [0001] The invention relates to a new method for preparing kombucha protein and its application, and belongs to the technical field of kombucha protein. Background technique [0002] Kombucha, also known as Haibao, Weibao, and kombucha, is a traditional folk acidic health drink with a long history. Because of its sweet and sour taste, it is still very popular among the public. Kombucha is made of sucrose and tea water as the fermentation substrate, and is co-fermented by yeast, acetic acid bacteria and lactic acid bacteria. Studies have shown that kombucha tea is a healthy drink that has multiple health effects on the human body. Such as clearing the stomach and intestines to help digestion, protecting the liver and kidneys and removing stones, promoting metabolism and increasing appetite, anti-aging, enhancing immunity, clearing the lungs and calming the nerves, preventing and treating atherosclerosis, etc. [0003] Although studies have shown that kombucha...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N13/00A61K8/64A61K8/04A61K8/9789A61Q19/00A61Q17/00A61K38/16A61P17/10A61P31/04
CPCC12N1/20C12N1/16C12N13/00A61K8/64A61K8/9789A61K8/042A61Q19/00A61Q17/005A61P17/10A61P31/04A61K2800/85A61K38/00Y02A50/30
Inventor 吴震孔令姗郑雄健谢振荣
Owner 上海澜海生物科技有限公司
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