Method for detecting anti-tuberculosis drug in serum by ultra-high performance liquid chromatography-tandem mass spectrometry technology
An ultra-high performance liquid chromatography and tandem mass spectrometry technology, which is applied in the field of ultra-high performance liquid chromatography tandem mass spectrometry detection of anti-tuberculosis drugs in serum, can solve the problems of unreachable drug concentration, high cost, cumbersome detection process, etc., and achieve simple Rapid detection method, accurate pretreatment process, high sensitivity effect
Inactive Publication Date: 2020-10-13
南京品生医学检验实验室有限公司
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Problems solved by technology
It is easy to cause the drug concentration in the body to be too low, so that the therapeutic drug concentration cannot be reached, or the drug concentration in the body is too high to cause toxic reactions. sex is more important
[0004] At present, the method for detecting the concentration of anti-tuberculosis drugs in the body is mainly ultra-high performance liquid chromatography-tandem mass spectrometry. Most of the reports in the literature are for the detection of 1-3 kinds of drugs at one time, and there are
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Embodiment 1
[0047] 1. Materials
[0048] (1) The samples of the methodological research experiment came from the serum samples collected from the outpatient clinic of Nanjing Drum Tower Hospital in August 2019.
[0049] (2) Instruments: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with automatic sampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA) ; ultrapure water instrument (ELGA LabWater, UK); multi-tube vortex mixer (Vortex genie2, US); adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glassware, Graduated cylinder, etc.
[0050] (3) Reagent consumables: MS grade methanol (Fisher, USA); HPLC grade methanol (Honeywell, USA); MS grade acetonitrile (Fisher, USA); HPLC grade acetonitrile (Honeywell, USA); MS grade formic acid (Fisher, USA) MS grade ammonium formate (Sigma, USA) chromatographic column: Phenomenex Kintex C18 (2.1×100mm, 2.6 μm) (Phenomenex, ...
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The invention discloses a method for detecting an anti-tuberculosis drug in serum by an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The antitubercular drug comprises cycloserine (CYS), pyrazinamide (PZN), isoniazid (INZ), p-aminosalicylic acid (P-ASA), ethiisonicotinamide (ETN), ethambutol (ETB), clofazimine (CFM), bedaquiline (BDQ), rifampicin (RFP), rifbutine (RFB) and rifapentine (RFT). The method includes: detecting the content of the antituberculosis drug in the pretreated serum by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry method, performing quantifying by utilizing a mass spectrometry isotope internal standard method, establishing a calibration curve by taking the concentration ratio of the standard substance to the internal standard substance as an X axis and the peak area ratio of the standard substance to the internal standard substance as a Y axis, and calculating the concentration of the target drug in the serum; the method is high in sensitivity, strong in specificity and simple in pretreatment process, separation and detection of the anti-tuberculosis drugs in serum are completed within 5 min, and a simple and rapid detection method is provided for clinical concentration monitoring of the anti-tuberculosis drugs.
Description
technical field [0001] The invention belongs to the technical field of analysis and detection, and more specifically relates to a method for detecting anti-tuberculosis drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Tuberculosis is an infectious disease caused by patients infected with Mycobacterium tuberculosis. So far, tuberculosis is still the infectious disease that causes the highest human mortality rate except AIDS. Anti-tuberculosis drugs are mainly divided into four categories: first-line anti-tuberculosis drugs, second-line anti-tuberculosis drugs, new anti-tuberculosis drugs, and compound drugs. [0003] In this patent, the first-line anti-tuberculosis drugs involved mainly include rifampicin (Rifamicin, RMP), isoniazid (Isoniazid, INH), pyrazinamide (Pvrazinamide, PZA) and ethambutol (Ethambutol, EMB) ; First-line anti-tuberculosis drugs are still the first choice for the treatment of tubercu...
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Login to view more IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7266G01N30/8679G01N2030/045G01N2030/067
Inventor 成晓亮李美娟
Owner 南京品生医学检验实验室有限公司
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