Selenium-rich pleurotus citrinopileatus mycelium polysaccharide as well as preparation and application thereof
A kind of mycelium polysaccharide, the technology of elm yellow mushroom, which is applied in the biological field
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Example 1: Preparation method of crude polysaccharide from selenium-enriched yellow mushroom mycelium
[0039] Selenium-enriched mycelium of ulmus chrysanthemum is obtained by liquid fermentation in shake flasks or fermentation tanks in our laboratory. The mycelium yield is more than 1.2g / 100mL, and the organic selenium content is greater than 100mg / kg mycelium. It is dried and crushed. Weigh 100g of dry powder, add hot water at 90°C according to the liquid-solid ratio of 15:1 (mL / g), extract at 90°C for 3h, cool to room temperature and centrifuge at 3500rpm for 15min, take the supernatant, and repeat the extraction of the residue once. Combine the two extracts, concentrate to 1 / 10 of the total volume, slowly add absolute ethanol to the concentrated solution until the final concentration of ethanol is 80%, leave it in the refrigerator at 4°C overnight, centrifuge at 3500rpm for 15min, discard The water extract of selenium-enriched ulm yellow fungus mycelium was obtained...
Embodiment 2
[0040] Example 2: Preparation method of homogeneous selenium-enriched yellow mushroom mycelia SPMP-1
[0041] Take 500 mg of the selenium-enriched yellow mushroom mycelia ((organic selenium content is 160-200 mg / kg)) polysaccharide crude product obtained in Example 1, fully dissolve in deionized water, and load the sample to the well-balanced DEAE Sephadex A-25 ion In the exchange chromatography column, the specification is (2.6×30cm), the balance liquid is deionized water, and the deionized water is used for elution at a flow rate of 1.5mL / min, collected in parts for 3min / tube, and the sulfuric acid-anthrone method is used to detect each The polysaccharide content in the tube eluent, the collection tube number as the abscissa, and the absorbance value as the ordinate draw the polysaccharide elution curve, and combine the same components according to the elution curve. Sephacryl S-200 molecular sieve chromatography on Sephacryl S-200 molecular sieve chromatography, eluting wit...
Embodiment 3
[0044] Example 3: Preparation method of homogeneous selenium-enriched yellow mushroom mycelium polysaccharide SPMP-2
[0045] Take 500 mg of the selenium-enriched yellow mushroom mycelium (organic selenium content is 120-130 mg / kg) polysaccharide crude product obtained in Example 1, fully dissolve it in deionized water, and load it on the well-balanced DEAE Sephadex A-25 ion exchange layer In the analysis column, the specification is (2.6×30cm), the balance liquid is deionized water, eluted with 1mol / L NaCl aqueous solution at a flow rate of 1.5mL / min, collected in sections for 3min / tube, and detected by sulfuric acid-anthrone method For the polysaccharide content in the eluate of each tube, take the collection tube number as the abscissa and the absorbance value as the ordinate to draw the polysaccharide elution curve, and combine the same components according to the elution curve. Sephacryl S-200 molecular sieve chromatography on Sephacryl S-200 molecular sieve chromatograph...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More