Method for detecting concentrations of anxiolytic and hypnotic drugs in serum by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

Abstract

The invention relates to a method for detecting anxiolytic and hypnotic drugs in serum by using an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The method can be used for detecting 11 kinds of anxiolytic and hypnotic drugs at one time, monitors target drugs and metabolites at the same time, is simple in pretreatment process, low in cost, high in sensitivity andhigh in specificity, can complete separation and detection of the anxiolytic and hypnotic drugs within 5 minutes, basically meets the requirements for accuracy degree and precision, can be used for quantitative analysis of the anxiolytic and hypnotic drugs clinically, and provides a simple and rapid detection method for monitoring the treatment concentrations of the anxiolytic and hypnotic drugsin serum clinically.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a method for detecting the concentration of anti-anxiety and hypnotic drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. The specific drugs are: Nitrazepam (NZP), Oxazepam ( Oxazepam (OXP), Estazolam (ESL), Temazepam (Temazepam, TMP), Alprazolam (Alprazolam, APL), Bromazepam (BZP), Lorazepam (LZP) ), midazolam (Midazolam, MDZ), zopiclone (Zopiclone, ZPC), diazepam (Diazapam, DZP) and zolpidem (Zolpidem, ZPM). Background technique [0002] Anxiety and insomnia is a common disease, which is generally affected by biological, psychological and social aspects. Benzodiazepines belong to a kind of psychotropic drugs, which have the effects of sedative, hypnotic, anxiolytic, anticonvulsant, and muscle relaxation. Benzodiazepines are drug-related benzodiazepine receptor agonists that exert a depressant effect on the central nervo...

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Problems solved by technology

[0009] At present, there are many patents for the detection of the concentration of anti-anxiety and hypnotic drugs in the body. For example, the Chinese patent application (publication number: CN 109085265 A) discloses a "liquid chromatography Kit and Application of Tandem Mass Spectrometry for Detecting Anxiolytic / Hypnotic Drugs in Serum and Plasma", 8 kinds of substances are detected at the same time, the sample volume needs to be 100 μL, and the pretreatment takes more than 35 minutes, Chinese patent application (publication number: CN 107991421 A) published "A liquid chromatographic analysis method for detecting the content of diazepam in blood", the sensitivity of diazepam is low, the limit of quantification is 0.625μg / mL, the sample volume needs to be 100μL, the pretreatment is cumbersome, extraction is required, nitrogen blowing is concentrated

In addition, there are patents for the detection of one or several of these drugs, which have relatively low efficiency, long analysis time for a single sample, low sensitivity, unsuitable linear range, complicated sample pretreatment, and matrix effect interference.

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