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Quantitative analysis method of berberine in blood plasma

A quantitative analysis, berberine technology, applied in the field of berberine analysis, can solve problems such as limiting the analytical capability of liquid chromatography mass spectrometry, and achieve the effects of easy automatic operation, ultra-clean extraction effect, and environmental protection.

Inactive Publication Date: 2020-11-13
苏州圣临医药科技有限公司
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Problems solved by technology

However, this method has a very strong matrix effect, which limits the maximum analysis ability of liquid chromatography mass spectrometry (LC-MS / MS). Even with Sciex6500, it can only achieve a sensitivity of 20.0pg / ml.

Method used

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  • Quantitative analysis method of berberine in blood plasma
  • Quantitative analysis method of berberine in blood plasma
  • Quantitative analysis method of berberine in blood plasma

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Embodiment 1

[0033] A method for quantitative analysis of berberine in plasma, the steps comprising: extracting 100 μL of plasma, using 100 μL of ammonia water with a concentration of 10% as a diluent to mix with the plasma, and mixing 10 μL of isotope internal standard to mix evenly, and then adding the mixed solution to the The 200mg filler extraction column (BIOTAGE) was implanted and adsorbed. After the pores of the filler were absorbed for 5 minutes, the berberine was washed out with 500μL chloroform to a 96-well collection plate and dried; Ammonium acetate was formulated as mobile phase at a volume ratio of 1:9 and mixed with the collected berberine, then 5 μL was injected for liquid chromatography-mass spectrometry analysis. The analysis results are shown in Tables 1-3 and Figure 1~5 .

[0034] Among them, the solid phase-supported liquid-liquid extraction method (SLE in English) is realized based on improved diatomite, which is a porous particle-type filler. Its porous structure m...

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Abstract

The invention discloses a quantitative analysis method of berberine in blood plasma. The method comprises the following steps: S1, carrying out pretreatment on plasma through a solid-phase supported liquid-liquid extraction method, wherein the method specifically comprises the following steps: diluting plasma, performing implantation adsorption in solid filler, eluting berberine by using an esterorganic solvent, drying, and finally uniformly mixing berberine in an analysis column by using an organic solvent mixed solution serving as a mobile phase to obtain an organic solvent mixed solution containing berberine; and S2, carrying out liquid-phase secondary mass spectrometry analysis on the organic solvent mixed solution by using a mass spectrometer. The method is easy and convenient to operate, automatic operation is easy, labor is saved, and the yield is high; the extraction effect is ultra-clean, and no latex liquid is formed; a large number of materials and equipment for traditionalextraction operation are omitted, cost is saved, and environmental protection is facilitated.

Description

technical field [0001] The invention relates to a method for analyzing berberine, in particular to a method for quantitatively analyzing berberine in plasma by using solid-phase supported liquid-liquid extraction (SLE) liquid chromatography mass spectrometry (LC-MS / MS). Background technique [0002] Berberine, also known as berberine, is a quaternary ammonium alkaloid isolated from Chinese medicine Coptis chinensis. It is the main active ingredient of Coptis chinensis antibacterial. It is widely distributed in the plant kingdom, and this product is found in about 4 families and 10 genera. [0003] Berberine has antibacterial effects on hemolytic streptococcus, Staphylococcus aureus, Neisseria gonorrhoeae, Freund's, Shigella, etc., and can enhance the phagocytosis of leukocytes, and has different degrees of inhibition on Mycobacterium tuberculosis and Yersinia pestis , It also has inhibitory effect on amoebic bacteria in rats. Berberine has anti-curare effect on animals, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045G01N2030/062
Inventor 潘炯伟卜海之田志轩
Owner 苏州圣临医药科技有限公司
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