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Construction method of phellinus igniarius fingerprint spectrum, fingerprint spectrum and application

A fingerprint and construction method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of outstanding uncertainty, large subjective influencing factors, and long time consumption, and achieve the effects of efficient screening, stable methods, and easy mastery

Active Publication Date: 2020-11-17
SHANGHAI ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Due to different parasitic tree species, cultivation methods and cultivation conditions, there are many varieties of Phellinus Phellinus and various forms. Now, the judgment of Phellinus Phellinus is still based on the appearance of the species, or some chemical indicators are determined through personal experience combined with simple chemical methods. (such as flavonoids, triterpenoids, etc.) to judge, the results are usually subject to relatively large factors, and the uncertainty is prominent, which will inevitably lead to misjudgment; biological analysis can also be used to judge, but it takes a long time and the cost is high , highly professional
At present, there is no efficient screening method for Phellinus cultivars

Method used

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  • Construction method of phellinus igniarius fingerprint spectrum, fingerprint spectrum and application

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preparation example Construction

[0048] In the present invention, the preparation method of the alcoholic extract of Phellinus radix preferably comprises the following steps:

[0049] Take the fruit body of Phellinus japonica and dry it at 48-52°C until the moisture content is lower than 10%, mix it with 80% ethanol aqueous solution at a solid-liquid ratio of 1:20g / mL, extract for 24h, filter, Concentrate and dry to obtain the alcohol extract of Phellinus luteum. In the present invention, before the processing of the Phellinus fruiting body, it is preferred to pulverize the Phellinus Phellinus fruiting body. In the present invention, after the drying, it is preferable to cut the fruiting body of Phellinus Phellinus into particles with a size of about 0.5 cm in length, width and height. In the present invention, the method of concentration is preferably a rotary evaporation concentration method. The drying method of the present invention is preferably a freeze-drying method. The present invention has no spe...

Embodiment 1

[0066] Construction of Phellinus Phellinus Fingerprint

[0067] 1 Collection of samples and preparation of Phellinus alcoholic extract

[0068] Phellinus fruiting bodies (all of which are poplar Phellinus spp.) samples were produced in Jinzhai, Anhui and Nanjing, Jiangsu, with four bacterial ages of 1, 2, 3, and 4 years, respectively (see Table 1 for details). Dry the collected Phellinus fruiting bodies at 50±2°C until the water content is lower than 10%, cut into particles with a size of about 0.5cm in length, width and height, extract 10g in 200mL80% ethanol solution for 24 hours, and filter to obtain the alcohol extract The solution was concentrated by rotary evaporation and freeze-dried for later use.

[0069] Table 1 Phellinus Phellinus fruiting body sample information

[0070]

[0071]

[0072] 2 High performance liquid chromatography (HPLC) analysis method

[0073] Sample preparation: Accurately weigh 20.0 mg of Phellinus lutea alcohol extract into a 1 mL centr...

Embodiment 2

[0092] Anti-tumor prediction model construction

[0093] 1 Inhibition of tumor cell growth test

[0094] Human breast cancer cells (MCF-7) and human liver cancer cells (HepG2) were selected to study the growth inhibition of tumor cells.

[0095] 32 samples of Phellinus ethanol extract were prepared into 40mg / mL, 20mg / mL, 10mg / mL, 2mg / mL, 1mg / mL and 0.4mg / mL sample solutions with DMSO solution under aseptic conditions, respectively, for later use.

[0096] Preparation of DMEM medium: add 100mL of fetal bovine serum to each liter of MEM medium, 10mL of 1% double antibody, 3mg / mL of penicillin, 5mg / mL of streptomycin, filter through a 0.22μm filter, and store at 4°C.

[0097] Take MCF-7 and HepG2 cells in the logarithmic phase, wash them with phosphate buffered solution (PBS), add 1 mL of trypsin, digest at 37°C for 2-3 minutes, first add 2 mL of DMEM medium to stop the digestion, and then according to the growth of the cells, , the two cells were diluted to 2 × 10 with DMEM me...

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Abstract

The invention relates to a phellinus igniarius fingerprint spectrum construction method, a phellinus igniarius fingerprint spectrum and application, and belongs to the technical field of edible and medicinal fungus fingerprint spectrum construction and spectrum effect research. The invention provides a phellinus igniarius fingerprint construction method, which comprises: taking a phellinus igniarius alcohol extract to prepare a detection sample, and carrying out high performance liquid chromatography analysis to obtain a mobile phase A which is a 0.01% acetic acid-aqueous solution and a mobilephase B which is acetonitrile, wherein the detection wavelength is 254 nm; and constructing a fingerprint spectrum according to a high performance liquid chromatography analysis result. According tothe construction method disclosed by the invention, efficient screening of different varieties of Sanghuangporous vaninii can be realized, and more specifically, the fingerprint spectrum obtained by the construction method disclosed by the invention can realize efficient screening of the Sanghuangporous vaninii.

Description

technical field [0001] The invention relates to the technical field of fingerprint construction and spectrum effect research of edible and medicinal fungi, in particular to a method for constructing Phellinus Phellinus fingerprint, fingerprint and application thereof. Background technique [0002] Phellinus belongs to Fungi Kingdom Fungi, Basidiomycota Basidiomycota, Hymenochaetales and Hymenochaetaceae, mainly including genera from Phellinus, Inonotus, Fomitiporia and Sanghuangporous. Known as "forest gold", it mainly grows on broad-leaved trees such as mulberry, poplar and willow. Most of them are yellow, and a few are brown or black, but they are all yellow when cut open. The growth distribution is concentrated in East Asia, Central Africa and North America. Phellinus has been recorded in many ancient books and medicines in our country. In traditional Chinese medicine, it mainly treats metrorrhagia, menstrual blood coagulation, postpartum hemagglutination in women, men's...

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N30/88
CPCG01N30/8686G01N30/88G01N2030/027G01N2030/8809Y02A50/30
Inventor 陈万超杨焱张俊峰张忠吴迪李文李正鹏张赫男张劲松冯娜陆本坤王琨
Owner SHANGHAI ACAD OF AGRI SCI
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