Disinfectant for medical institution wastes and preparation method thereof
A technology of medical institutions and disinfectants, applied in botany equipment and methods, chemicals for biological control, disinfectants, etc., can solve the problems of toxic high-carbon steel sheets, etc., and achieve the effect of reducing impact and simple preparation process
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[0029] 2. Preparation of spore suspension
[0030] Inoculate Bacillus subtilis on LB slant and culture for 72-96 hours, rinse with sterile saline to obtain bacterial suspension, suck the bacterial suspension into a sterilized conical centrifuge tube, inactivate the bacterial cells in a 75°C water bath for 30 minutes, and centrifuge for 10 minutes (500r / min), discard the sediment at the bottom of the tube, and observe it as a bacterial suspension containing spores through microscopic examination and staining. When the spore formation rate reaches more than 95%, follow-up experiments can be carried out. The spore suspension can be stored at 4°C for use, and the validity period is half a year. When the spore suspension is used, the live bacteria culture count should be carried out first.
[0031] 3. Preparation of disinfectant:
Embodiment 1
[0033] A disinfectant for waste in medical institutions, the raw material composition and mass ratio of the effective components of the bactericidal disinfectant are: 0.1% glutaraldehyde, 0.1% dodecyl dimethyl benzyl ammonium chloride, Didecyl dimethyl ammonium chloride 0.15%, sodium nitrite 1%, isopropanol 0.1%, camphor oil 0.01%, and the rest is distilled water; pH is adjusted to 7.0 by sodium bicarbonate.
[0034] A preparation method for a disinfectant for medical institution waste, comprising the following preparation steps:
[0035] S1: Measure or weigh the required materials according to the ratio requirements of each component;
[0036] S2: Pour a quantitative isopropanol solution through the drain, then pour dodecyldimethylbenzyl ammonium chloride through the drain, stir and mix at a low speed, and let stand;
[0037] S3: Pour into the weighed didecyl dimethyl ammonium chloride, stir and mix at a low speed, and let stand;
[0038] S4: Pour in the weighed glutaraldeh...
Embodiment 2
[0044] A disinfectant for waste in medical institutions. The composition and mass ratio of raw materials for making the active ingredients of the disinfectant are: 0.5% glutaraldehyde, 0.1% dodecyldimethylbenzyl ammonium chloride, bis Decyl dimethyl ammonium chloride 0.1%, sodium nitrite 1.5%, isopropanol 0.05%, camphor oil 0.05%, and the rest is distilled water.
[0045] The preparation method of the disinfectant in this embodiment is the same as that of Embodiment 1.
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