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Periplaneta americana polypeptide extraction method

A technology of Periplaneta americana and its extraction method, which is applied in the field of extraction and preparation of natural components, can solve the problems such as the smoothness of the emulsification process of the extract, and achieve the effects of avoiding oil separation failure, ensuring the smoothness of the process, and increasing the content of polypeptides

Active Publication Date: 2020-11-20
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of Periplaneta americana polypeptide extraction method, this extraction method adopts the polarity increasing extraction method to remove most of grease, in order to solve the emulsification of extracting solution and poor process smoothness in the Periplaneta americana extraction process technical problem

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  • Periplaneta americana polypeptide extraction method

Examples

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Effect test

Embodiment 1

[0035]S1 extraction step: take 5 kg of Periplaneta americana medicinal material, and pulverize it below 0° C. into coarse powder (through a 20-mesh sieve) to obtain Periplaneta americana coarse powder. Adding the volume fraction of Periplaneta americana coarse powder is 90% ethanol solution and soaks four times, uses 25L ethanol solution for soaking each time, soaks 24h, filters and gets filtrate after each soaking finishes, and the filtrate that soaks for four times is obtained merges, and then The combined filtrates were concentrated to one-half of the original volume using a rotary evaporator to obtain a crude extract (about 45 L in volume).

[0036] S2 extraction step: performing a total of N stages of extraction on the crude extract, where N≥2, where N is an integer;

[0037] In the first-stage extraction, the crude extract is extracted with a non-polar organic solvent, and the alcohol layer is taken to obtain the first-stage extract; in the n-stage extraction, the corres...

Embodiment 2

[0048] This embodiment is basically the same as Embodiment 1, the difference is:

[0049] S1 extraction step: take 10 kg of Periplaneta americana medicinal material raw material, freeze to -10°C, and grind it into a 20-mesh powder with a grinder (keep below 0°C during the grinding process) to obtain Periplaneta americana coarse powder. The extraction solvent used was methanol (pure), soaked and extracted four times, the amount of methanol used for each soak was 100 L, the soaking system was stirred each time, and the duration of each extraction was 2 hours. After combining the filtrates, the volume of the combined filtrates was concentrated to one-half of the original volume to obtain a crude extract (about 200 L in volume).

[0050] S2 extraction step: the organic solvent used is petroleum ether, and the amount of petroleum ether used in each extraction is about 70 L in the first-stage extraction to the fourth-stage extraction. In the 2nd stage extraction, 3rd stage extracti...

Embodiment 3

[0054] This embodiment is basically the same as Embodiment 1, the difference is:

[0055] S1 extraction step: get 20kg of Periplaneta americana medical material raw material, the extraction solvent that uses is the methanol solution that volume fraction is 95%, carries out soaking and extracting four times under the condition of 50 ℃, the methanol solution (95%) that each soaking extracts uses The volume is 120L, and the time for each soaking and extraction is 4h. After combining the filtrates, the volume of the combined filtrates was concentrated to one-half of the original volume to obtain a crude extract (about 240 L in volume).

[0056] S2 extraction step: the organic solvent used is pentane, which only contains three stages of extraction. From the first stage to the third stage extraction, the amount of pentane used for each extraction is 80L. In the second stage extraction and the third stage extraction, the amount of pure water added is 24L and 48L respectively.

[00...

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Abstract

The invention belongs to the technical field of natural component extraction and preparation, and relates to a periplaneta americana polypeptide extraction method. The method comprises the following steps of removing most of grease by adopting a solvent polarity progressive increase extraction method, and then removing a small amount of residual grease, solvent residues and pigments by adopting aresin adsorption method. In an obtained polypeptide product, the grease residues and organic solvent residues used in the technological process cannot be detected, the fishy smell in the product is eliminated, the content of the product is increased, and the product quality is greatly improved. The method has relatively good market application prospects and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of natural components, and relates to a method for extracting Periplaneta americana polypeptide. Background technique [0002] Periplaneta americana, also known as Feilian, has been recorded in "Shen Nong's Materia Medica". Modern research shows that its main active ingredients are polypeptides and biological enzymes, which have obvious effects of promoting tissue repair and regeneration, anti-tumor and anti-hepatitis. The marketed drugs with Periplaneta americana as the main raw material include Kangfuxin Liquid, Xinmailong, and Ganlong Capsules, etc., which have produced good economic and social benefits. [0003] At present, the active ingredient polypeptide of Periplaneta americana usually uses high-concentration alcohol as the solvent medium to extract, then adds water to the relative system to separate, and finally uses activated carbon and macroporous resin to absorb the ...

Claims

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Application Information

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IPC IPC(8): C07K1/14A61K35/64
CPCC07K1/145A61K35/64Y02A50/30
Inventor 马晓霞刘录王越函丁雄蒲世彪谭文红周志宏
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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