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Antibacterial repair plant extract composition, and preparation method and application thereof

A technology for extracting and repairing plants, which can be applied in pharmaceutical formulations, cosmetic preparations, and dressing preparations. It can solve problems affecting product safety, organic solvent residues, and limited scope of application. Liquid stability, good antibacterial effect

Active Publication Date: 2020-11-24
COSMAX CHINA INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the organic solvent extraction process is applied to large-scale production, there is a risk of organic solvent residues, which will affect the safety of the product, so the scope of application of this method is limited

Method used

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  • Antibacterial repair plant extract composition, and preparation method and application thereof
  • Antibacterial repair plant extract composition, and preparation method and application thereof
  • Antibacterial repair plant extract composition, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 antibacterial plant extract composition

[0041] In the following examples and comparative examples of the present invention, the bacteriostatic plant extract composition was prepared by the following process.

[0042] (1) Crush Forsythia, Houttuynia cordata, and Radix Radix respectively, and mix well;

[0043] (2) adding deionized water for extraction, the mass ratio of raw material drug to deionized water is 1:30, the extraction temperature is 90°C, the extraction time is 1.5h, and the extraction frequency is 2 times;

[0044] (3) cooling the extract obtained in step (2) to 25° C., filtering with 200 mesh gauze to obtain the initial filtrate;

[0045] (4) adding clarifier chitosan to the primary filtrate obtained by step (3), and standing at low temperature for 8h;

[0046] (5) Centrifuge the solution obtained in step (4) for 40min, the rotating speed is 5000rpm, filter with a microfiltration membrane with a pore size of 0.2 μm, collect...

Embodiment 2

[0054] Embodiment 2 antibacterial effect detection

[0055] Selection of strains: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Propionibacterium acnes; Pseudomonas aeruginosa \ Staphylococcus aureus \ Escherichia coli \ Propionibacterium acnes were selected for the preparation of bacterial solution for the listed species. Preparation: Dilute it with sterile saline to about 1.0x10 5 cfu / ml~1.0x10 6 cfu / ml bacterial suspension. The prepared bacterial suspension should be used within 2 hours, or stored at 2°C to 8°C for no more than 24h.

[0056] Bacteriostatic effect detection: take the functional plant composition obtained in step (7) of Example 1, take a piece of sterile dry filter paper, and add 20 μL of the plant composition solution (10 mg / mL) of the actual concentration to each piece dropwise. Test bacteria inoculation: Take 1mL of each test bacteria suspension and spread evenly on the surface of the nutrient agar medium; paste the antibacterial tes...

Embodiment 3

[0063] Example 3 Cytotoxicity Evaluation Test:

[0064] MTT assay is an experimental method widely used in the detection of cytotoxicity or cell proliferation by measuring the number of living cells. The detection principle is that succinate dehydrogenase (succinate dehydrogenase or mitochondrial dehydrogenase) in living cell mitochondria can make water-soluble The yellow salt MTT (3-[4,5-dimethlythiazole-2-yl]-2,5-diphenyl tetrazolium bromide) is reduced to a water-insoluble blue color and deposited in cells, while dead cells do not have this function. The generated crystalline formazan (formazan) is generally dissolved by adding DMSO (dimethlysulfoxide) to measure the absorbance. The specific experimental method is in 96well multi plate (corning) according to 1X10 4 DMEM medium containing 10% bovine serum and 100 μL of keratinocytes (HaCaT) were inoculated at the density of cells / well, and replaced with serum-free medium after 24 hours of culture. Samples of different con...

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Abstract

The invention provides an antibacterial repair plant extract composition, a composition and a preparation method and application thereof. The plant extract composition is prepared from the following ingredients in percentage by mass: fructus forsythia, herba houttuyniae and radix isatidis according to a ratio of (1 to 6.5) to (1 to 6.5) to (1 to 6.5). The plant extract composition is prepared by the processes such as crushing, extraction, concentration, formulating and sterilization, and common pathogenic bacteria can be obviously inhibited. The raw materials are pure natural plants; all ingredients achieve a good synergistic effect; obvious antibacterial and repairing effects are achieved; the property is mild; the absorption is easy; no irritation exists; and the antibacterial repair plant extract composition specially meets the requirements of people on safety, effectiveness and zero burden of cosmetics, and can be widely applied to skin care cosmetics.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to a plant extract composition with antibacterial and repairing effects, a preparation method and application thereof. Background technique [0002] The new crown epidemic is coming fiercely, and with it, the market demand for antibacterial products has increased significantly. Although the current epidemic situation is gradually improving, the pressure of "internal prevention of recurrence and external prevention of importation" has made antibacterial and disinfection products an indispensable daily necessities for people in the post-epidemic era. On the other hand, with the improvement of living standards and the enhancement of health awareness, people pay more and more attention to the green safety and functionality of medicines and cosmetics. Natural and healthy plant extract products are welcomed by more and more consumers. Respected, and the market is expanding step by st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q17/00A61Q19/00
CPCA61K8/9789A61Q17/005A61Q19/00A61K2800/5922A61K2800/805Y02A50/30
Inventor 金荣熙陈玉琳周春亚齐秋月周秋娜魏茂林金炫延金延埈
Owner COSMAX CHINA INC
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