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Application of nicotinamide mononucleotide in preparation of medicine for preventing, improving and/or treating polycystic ovarian syndrome

A single nucleotide and nicotinamide technology, applied in the field of biomedicine, can solve the problems of no clear standard for the course of treatment, low compliance, and difficulty in effectively improving patients' symptoms

Inactive Publication Date: 2020-11-24
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese PCOS experts recommend short-acting oral contraceptives (Diane-35 is the first choice) for the treatment of hyperandrogenism, but there is no clear standard for the course of treatment; PCOS patients also have abnormal glucose and lipid metabolism, and metformin is only used for diabetes in domestic guidelines. Patients with abnormal tolerance, and pregnant women are not listed as suitable groups; surgical treatment, but postoperative pelvic adhesions and premature ovarian failure are prone to occur; in today's fast-paced lifestyle, standard diet and physical exercise have low compliance and are difficult Effectively improve patient symptoms

Method used

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  • Application of nicotinamide mononucleotide in preparation of medicine for preventing, improving and/or treating polycystic ovarian syndrome
  • Application of nicotinamide mononucleotide in preparation of medicine for preventing, improving and/or treating polycystic ovarian syndrome
  • Application of nicotinamide mononucleotide in preparation of medicine for preventing, improving and/or treating polycystic ovarian syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of PCOS-IR rat model

[0056] Five-week-old SD rats were randomly divided into a control group and a model group after one week of adaptive feeding. The model group was treated with trazole 1 mg / kg·d, dissolved in 1% sodium carboxymethyl cellulose (CMC ), continuously gavage for 30 days, while being fed with high-fat feed. The control group was fed with ordinary feed. After 20 days of administration, the pictures of the vagina of SD rats were monitored, and the model was initially determined based on the estrus cycle. The SD rats with successful model construction were divided into PCOS group (model group) and NMN group (experimental group).

Embodiment 2

[0057] Example 2 Preparation of vaginal smear

[0058] The rat was fixed in the supine position, and 20 μL of sterile normal saline was sucked with a pipette, and the normal saline was injected into the vagina about 1 cm. The pipette was repeatedly blown and sucked 3 times, and 10 μL of liquid was sucked out and smeared on the slide evenly, and dried. Add 1-2 drops of Wright-Giemsa stain solution to each slide. After 2 minutes, add the same dose of 0.01M disodium hydrogen phosphate buffer. Shake the slide to make the two liquids evenly mixed. Let stand for 10 minutes. Rinse the dye solution with distilled water. After drying, the xylene dye solution is transparent and sealed with neutral resin for microscopic inspection.

Embodiment 3

[0059] Example 3 Blood sampling from the abdominal aorta and ovarian and pancreatic tissue sampling

[0060] The rats were treated after the expiration of the administration period and fasted for 12 hours before treatment. All rats were weighed, and blood was collected from the tail vein to measure fasting blood glucose. Chloral hydrate was used for intraperitoneal anesthesia (6% concentration, 0.6-0.7ml / 100g body weight). After successful anesthesia, fix the rat on the dissection table in a supine position. Shave the abdomen. After disinfection, cut it layer by layer along the main midline of the rat's abdomen with scissors. After opening the abdominal cavity, look for the abdominal aorta and abdominal aorta Located in front of the spine and beside the abdominal vena cava (thicker and darker in color than the abdominal aorta), use forceps or hemostatic forceps to gently separate the adipose tissue and fascia tissue around the blood vessels to clearly expose the blood vessels. ...

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Abstract

The invention relates to the field of biological medicines, in particular to an application of nicotinamide mononucleotide in preparation of a medicine for preventing, improving and / or treating the polycystic ovarian syndrome (PCOS). A PCOS rat model is constructed by combining letrozole with high-fat feed, the protective effect of the nicotinamide mononucleotide on the PCOS is studied, and experimental results show that nicotinamide mononucleotide intervention can obviously improve the follicular development condition of PCOS rats, increase the number of corpus luteum, thicken granular cell layers, restore the oestrus cycle of the rats, reduce body weight and fasting blood sugar, reduce serum androgen, luteinizing hormone, insulin and insulin resistance index level, increase secretion oflactic acid and ATP, and improve ovarian energy metabolism.

Description

Technical field [0001] The present invention relates to the field of biomedicine, in particular to the application of nicotinamide mononucleotide in the preparation of drugs for preventing, improving and / or treating cystic ovarian syndrome. Background technique [0002] Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women of puberty and childbearing age, and is the main cause of women's irregular menstruation and infertility. Among women of childbearing age, the prevalence of PCOS is 5-10%, which is the main cause of ovulatory infertility, the proportion is as high as 75%, and the incidence of early spontaneous abortion in PCOS is 20-41%. The main clinical manifestations are hyperandrogenemia, sparse ovulation or anovulation, polycystic ovary, menstrual disorders, insulin resistance, dyslipidemia, hirsutism, acne, etc. PCOS is also prone to type 2 diabetes, cardiovascular disease, depression, anxiety, etc. It can be seen that PCOS is a multi-sys...

Claims

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Application Information

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IPC IPC(8): A61K31/706A61P15/00A61P15/08A61P5/28A61P5/48
CPCA61K31/706A61P5/28A61P5/48A61P15/00A61P15/08
Inventor 雷小灿刘慧晴张顺李素云陈熙李美香黄兰娥梁爱红
Owner NANHUA UNIV
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