Paper diaper and preparation method thereof
A technology of diapers and hyaluronic acid, applied in the field of hygiene products, can solve the problems of skin microecological disorders, harmful skin, irritation of baby skin, etc., and achieve the effects of inhibiting growth, promoting growth and moisturizing skin
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preparation example Construction
[0092] In the present application, the preparation method of the powder comprises:
[0093] Dissolving hyaluronic acid, ectoine, mannitol, and Sclerotinia gelatin in lactic acid bacteria fermentation filtrate to obtain a mixed solution;
[0094] The mixed solution is filtered and sterilized and then dried to obtain powder. In this application, filter sterilization is to use 0.22 μm pore size filter membrane to filter.
[0095] Specifically, one of the lactic acid bacteria fermentation filtrates in this application is: plantarum lactobacillus preserved on a slant is inoculated into a liquid MRS medium for cultivation, cultured at 37°C for 24 hours, and the activated seeds are inoculated with 5% inoculum In the fermentation medium, ferment at 37°C for 48 hours. After the residual sugar is exhausted, sterilize with a 0.22 μm filter membrane to obtain a lactic acid bacteria fermentation filtrate.
[0096] The fermentation medium is as follows: the carbon source is one or more of...
Embodiment 1
[0138] Lactic acid bacteria fermentation filtrate is:
[0139] The Lactobacillus plantarum preserved on the slant was inoculated into the liquid MRS medium for cultivation, cultured at 37°C for 24 hours, the activated seeds were inoculated into the fermentation medium with 5% inoculum, left to ferment at 37°C for 48 hours, and the residual sugar consumption After exhaustion, filter and sterilize to obtain the lactic acid bacteria fermentation filtrate.
[0140] The fermentation medium is: carbon source is glucose 25g / L; nitrogen source is yeast powder 5g / L, peptone 10g / L; inorganic salt is disodium hydrogen phosphate 1g / L, sodium dihydrogen phosphate 1g / L, sodium acetate 5g / L L; the trace element is vitamin B2 10mg / L, and the pH is natural.
[0141] The solid product of sclerotinium gelatin is produced by Bloomage Biotechnology Co., Ltd., with a molecular weight of >2 million.
[0142] The superabsorbent resin is prepared from starch grafted with sodium polyacrylate, which i...
experiment example 1
[0158] Microbial Distribution Experiment
[0159] 105 infants were recruited, aged between 12 and 24 months, divided into 21 groups, respectively 1-21 groups, 5 people in each group, 21 groups of volunteers respectively used examples 1 to 17, and comparative examples 1 to 1 every day. For the diapers of 4, use them for 0 days, 7 days, and 14 days, and stop using them for 7 days. Use a sterile cotton swab to take samples from the skin that touches the diapers, place the samples in sterile water after mixing, and apply 100 μL each on the In Staphylococcus aureus selective screening medium, Escherichia coli selective medium, and lactic acid bacteria selective medium, the total number of colonies after cultivation, and the average test results of the total number of colonies are shown in the table below. The more colonies, the more microorganisms on the skin more.
[0160] Staphylococcus aureus differential medium:
[0161] High Salt Mannitol Agar (g / L)
[0162] Peptone 10, bee...
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