Tremella polysaccharide and its preparation method and application
A technology of tremella polysaccharide and tremella powder, which is applied in the fields of pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., and can solve the problems of low sugar yield, low process efficiency, and large loss of polysaccharides, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0061] The present embodiment provides a kind of preparation method of white fungus polysaccharide, and specific process is as follows:
[0062] (1) Dry the commercially available white fungus in the sun, take the dried white fungus, remove impurities, crush through an 80-mesh sieve, take 100 g of white fungus powder, and add 0.15 mol / L of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and then heated in a water bath at a temperature of 70°C for 3 h to obtain a crude extract;
[0063] (2) Cool the crude extract to 50°C, adjust the pH to 4.7, add pectin-cellulose complex enzyme (the mass ratio of pectinase to cellulase is 1.75:1), and add 0.5% of the mass of white fungus powder %, enzymolysis reaction 4 h;
[0064] (3) After enzymatic hydrolysis, use filter cloth to filter 3 times, 200 mesh filter cloth for the first time, 200 mesh filter cloth for the second time, and 700 mesh filter cloth for the third time, take the filtrate, and centrifuge at 7500...
Embodiment 2
[0067] The present embodiment provides a kind of preparation method of white fungus polysaccharide, and specific process is as follows:
[0068] (1) Dry the commercially available white fungus, take the dried white fungus, remove impurities, crush through an 80-mesh sieve, take 100 g of white fungus powder, and add 0.2 mol / L of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and then heated in a water bath at a temperature of 80°C for 2 h to obtain a crude extract;
[0069] (2) Cool the crude extract to 40°C, adjust the pH to 5.2, add pectin-cellulose complex enzyme (the mass ratio of pectinase to cellulase is 1.5:1), and add 0.2% of the mass of white fungus powder %, enzymolysis reaction 6 h;
[0070] (3) After enzymatic hydrolysis, filter with filter cloth for 3 times, 100-mesh filter cloth for the first time, 300-mesh filter cloth for the second time, and 500-mesh filter cloth for the third time. Take the filtrate and centrifuge at 8000 rpm / min for...
Embodiment 3
[0073] The present embodiment provides a kind of preparation method of white fungus polysaccharide, and specific process is as follows:
[0074] (1) Dry the commercially available white fungus, take the dried white fungus powder, remove impurities, crush through an 80-mesh sieve, take 100 g white fungus powder, add 0.1 mol / L to the white fungus powder at a material-to-liquid ratio of 1:40 g / mL disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and then heated in a water bath at a temperature of 60°C for 4 h to obtain a crude extract;
[0075] (2) Cool the crude extract to 60°C, adjust the pH to 4.2, add pectin-cellulose compound enzyme (the mass ratio of pectinase to cellulase is 2:1), and the added amount is 0.8% of the mass of white fungus powder %, enzymatic hydrolysis reaction 2 h;
[0076] (3) After enzymatic hydrolysis, use filter cloth to filter 3 times, 1 time with 150 mesh filter cloth, the second time with 250 mesh filter cloth, and the third t...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com