Tremella polysaccharide and its preparation method and application

A technology of tremella polysaccharide and tremella powder, which is applied in the fields of pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., and can solve the problems of low sugar yield, low process efficiency, and large loss of polysaccharides, etc.

Active Publication Date: 2021-05-18
广州善合化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN106117387A discloses a preparation method of low molecular weight white fungus polysaccharide, by mixing white fungus powder with water, adjusting the pH to alkaline, extracting to obtain a crude extract; adjusting the temperature and pH of the crude extract, adding enzymes for enzymolysis ; The product obtained after enzymolysis is subjected to solid-liquid separation, the filtrate is taken, concentrated, and a paste is obtained; ethanol and H are added to the paste 2 o 2 Carry out polysaccharide degradation, static precipitation, centrifugation, washing, and drying to obtain low-molecular-weight white fungus polysaccharides in which polysaccharides with a molecular weight below 10,000 Daltons account for more than 95% of the total polysaccharides, but the sugar yield is low
[0005] CN102161710A discloses a preparation method of low-molecular-weight white fungus polysaccharides. The preparation method is to clean and homogenize white fungus, add acid aqueous solution, extract at a certain temperature and pressure for 1 to 3 hours, and then use alkaline water to dissolve the acid water The extract is neutralized to neutral, centrifuged, the supernatant is concentrated to an appropriate volume, and finally the ultrafiltration membrane method is adopted to obtain low molecular weight tremella polysaccharides, but the ultrafiltration membrane process causes a large loss of polysaccharides and low process efficiency

Method used

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Experimental program
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Effect test

Embodiment 1

[0061] The present embodiment provides a kind of preparation method of white fungus polysaccharide, and specific process is as follows:

[0062] (1) Dry the commercially available white fungus in the sun, take the dried white fungus, remove impurities, crush through an 80-mesh sieve, take 100 g of white fungus powder, and add 0.15 mol / L of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and then heated in a water bath at a temperature of 70°C for 3 h to obtain a crude extract;

[0063] (2) Cool the crude extract to 50°C, adjust the pH to 4.7, add pectin-cellulose complex enzyme (the mass ratio of pectinase to cellulase is 1.75:1), and add 0.5% of the mass of white fungus powder %, enzymolysis reaction 4 h;

[0064] (3) After enzymatic hydrolysis, use filter cloth to filter 3 times, 200 mesh filter cloth for the first time, 200 mesh filter cloth for the second time, and 700 mesh filter cloth for the third time, take the filtrate, and centrifuge at 7500...

Embodiment 2

[0067] The present embodiment provides a kind of preparation method of white fungus polysaccharide, and specific process is as follows:

[0068] (1) Dry the commercially available white fungus, take the dried white fungus, remove impurities, crush through an 80-mesh sieve, take 100 g of white fungus powder, and add 0.2 mol / L of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and then heated in a water bath at a temperature of 80°C for 2 h to obtain a crude extract;

[0069] (2) Cool the crude extract to 40°C, adjust the pH to 5.2, add pectin-cellulose complex enzyme (the mass ratio of pectinase to cellulase is 1.5:1), and add 0.2% of the mass of white fungus powder %, enzymolysis reaction 6 h;

[0070] (3) After enzymatic hydrolysis, filter with filter cloth for 3 times, 100-mesh filter cloth for the first time, 300-mesh filter cloth for the second time, and 500-mesh filter cloth for the third time. Take the filtrate and centrifuge at 8000 rpm / min for...

Embodiment 3

[0073] The present embodiment provides a kind of preparation method of white fungus polysaccharide, and specific process is as follows:

[0074] (1) Dry the commercially available white fungus, take the dried white fungus powder, remove impurities, crush through an 80-mesh sieve, take 100 g white fungus powder, add 0.1 mol / L to the white fungus powder at a material-to-liquid ratio of 1:40 g / mL disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, and then heated in a water bath at a temperature of 60°C for 4 h to obtain a crude extract;

[0075] (2) Cool the crude extract to 60°C, adjust the pH to 4.2, add pectin-cellulose compound enzyme (the mass ratio of pectinase to cellulase is 2:1), and the added amount is 0.8% of the mass of white fungus powder %, enzymatic hydrolysis reaction 2 h;

[0076] (3) After enzymatic hydrolysis, use filter cloth to filter 3 times, 1 time with 150 mesh filter cloth, the second time with 250 mesh filter cloth, and the third t...

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PUM

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Abstract

The invention discloses a white fungus polysaccharide and its preparation method and application. The preparation method comprises: (1) extracting white fungus powder with a neutral buffer solution to obtain an extraction material liquid; (2) adding the extraction material liquid to Add pectin cellulose compound enzyme to the compound enzyme for enzymolysis, the mass ratio of pectinase and cellulase in the pectin cellulose compound enzyme is (1.5~2):1; (3) filter after enzymolysis, take The filtrate is centrifuged, and the supernatant is taken after standing still; (4) the supernatant is subjected to alcohol precipitation and centrifuged, and the obtained precipitate is tremella polysaccharide. The preparation method of the present invention has low cost and high efficiency, and the yield of tremella polysaccharide is higher than 40%, which is suitable for large-scale commercial production. The tremella polysaccharide prepared by the method has polysaccharides with a molecular weight below 10000Da accounting for more than 95% of the total polysaccharides , the total sugar content is greater than 92%, and has broad application prospects in the fields of food, medicine, health care products and cosmetics preparation.

Description

technical field [0001] The invention belongs to the technical field of tremella polysaccharide preparation, and relates to tremella polysaccharide and its preparation method and application. Background technique [0002] Tremella polysaccharide is the main active ingredient of Tremella, including acid polysaccharides, neutral polysaccharides, neutral heteropolysaccharides, acid oligosaccharides, cell wall polysaccharides and extracellular polysaccharides, etc. Immunity and the effects of removing chloasma are widely used in the fields of food, health products and cosmetics. Tremella polysaccharide with large molecular weight has good moisturizing ability and excellent film-forming properties, and has anti-oxidation, anti-allergic and anti-inflammatory effects. However, it has the disadvantages of low solubility, difficulty in being absorbed by the skin, and sticky feeling when applied in formulations, and there are certain defects in the application in cosmetics. Tremella ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K8/73A61Q19/00
CPCA61K8/73A61Q19/00C08B37/0003
Inventor 陈云波
Owner 广州善合化工有限公司
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