Photonic crystal sensing material for SEB ultra-sensitive detection, preparation method thereof and method for ultra-sensitive detection of SEB

A technology of photonic crystals and sensing materials, which is applied in the direction of material analysis, analysis materials, and measuring devices through optical means, can solve the problems of complicated operation procedures, long time required for the detection process, and inability to meet fast and convenient detection, etc., to achieve Convenient operation, improved biocompatibility, and wide detection range

Pending Publication Date: 2021-01-08
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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Problems solved by technology

[0003] At present, detection methods with high sensitivity and reliable results often rely on expensive experimental equipment, the ope

Method used

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  • Photonic crystal sensing material for SEB ultra-sensitive detection, preparation method thereof and method for ultra-sensitive detection of SEB
  • Photonic crystal sensing material for SEB ultra-sensitive detection, preparation method thereof and method for ultra-sensitive detection of SEB
  • Photonic crystal sensing material for SEB ultra-sensitive detection, preparation method thereof and method for ultra-sensitive detection of SEB

Examples

Experimental program
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Effect test

Example Embodiment

[0034] Example 1

[0035] Preparation of Photonic Crystal Sensing Materials

[0036] (1) Add 150 mL of absolute ethanol and 9 mL of ammonia water to the washed dry flask and mix, then add 7.2 mL of tetraethyl orthosilicate (TEOS), stir vigorously at 30°C, and react at 600 rpm for 9 hours; After the end, the remaining ammonia water and dehydrated alcohol are removed by centrifugal method (rotating speed 4000rpm), then redispersed in dehydrated alcohol, and the particle size is separated by centrifugal sieving (800rpm to get supernatant, 1500rpm to get precipitation), and finally the obtained The colloidal particles (silicon spheres) were dispersed in 10 mL of absolute ethanol and stored;

[0037] (2) Take 1 mL of the synthesized silicon ball and dilute it to 10 mL with absolute ethanol, place it in a 50 mL flask, add 200 μL of 3-aminopropyltriethoxysilane (APTES) and sonicate for 5 min, then fill with nitrogen and remove oxygen 30min, and finally mechanically stirred for 6 ho...

Example Embodiment

[0041] Example 2

[0042] Check the drawing of the working curve

[0043] (1) Soak the photonic crystal sensing material in PBS buffer (pH is 7.4), fix the probe of the optical fiber spectrometer vertically above the photonic crystal and immerse it under the liquid surface, and obtain the reflection spectrum of the photonic crystal;

[0044] (2) After the system is stabilized, SEB with different concentrations is added in sequence, so that the SEB concentration of the system becomes 0.01 fg / mL ~ 1 μg / mL, and the stabilized reflection peaks are recorded respectively;

[0045] (3) Taking the logarithm of SEB concentration as the abscissa, and the variation of the reflection peak intensity as the ordinate, establish a working curve. like Figures 3a-3c shown, Figure 3a The response of the Aptamer-PC sensing material to SEB is shown; Figure 3b The relationship between the logarithm of SEB concentration and the change in reflection peak intensity is shown; Figure 3c A stand...

Example Embodiment

[0046] Example 3

[0047] (1) Milk sample, centrifuged at 8000rpm for 15 minutes to remove fat, and then diluted 500 times with PBS buffer;

[0048] (2) Add different concentrations of SEB standards to the above-mentioned treated samples respectively, to make them spiked samples of different concentrations (0.1pg / mL, 1pg / mL and 100pg / mL);

[0049] (3) The above-mentioned spiked samples are sequentially added to the sensing system, and after stabilization, the intensity change of the reflection peak is recorded, and the spiked recovery rate is calculated. The results are shown in Table 1 below:

[0050] Table 1 Detection results of SEB in milk samples

[0051]

[0052] Note: N.D.* means SEB not detected.

[0053] It can be seen from the results in Table 1 that the recovery rate of standard addition of the milk sample is 100.63% to 114.98%, indicating that the method of the present invention has better accuracy.

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Abstract

The invention belongs to the field of toxin detection, and relates to a photonic crystal sensing material for SEB ultra-sensitive detection, a preparation method of the photonic crystal sensing material and a method for ultra-sensitive detection of SEB. The preparation method comprises the following steps: carrying out hydrolysis reaction on tetraethyl orthosilicate to obtain silicon spheres; mixing and reacting the silicon spheres with 3-aminopropyl triethoxysilane to obtain aminated silicon spheres; carrying out contact reaction on an aqueous solution of aminated silicon spheres and a nanogold solution to obtain aminated silicon spheres SiO2-AuNPs modified with nanogold particles, preparing a SiO2-AuNPs microsphere suspension, performing drying in a constant-temperature drying oven, andperforming stacking to obtain photonic crystals; and adding the SEB aptamer of which the 5'end is modified with sulfydryl into the SiO2-AuNPs photonic crystal, and performing incubating to obtain theSEB aptamer-photonic crystal sensing material. The photonic crystal sensing material provided by the invention can realize ultrasensitive and label-free detection of SEB.

Description

technical field [0001] The invention belongs to the field of toxin detection, and in particular relates to a preparation method of a photonic crystal sensing material for SEB ultrasensitive detection, the photonic crystal sensing material for SEB ultrasensitive detection prepared by the preparation method, and A method for ultrasensitive detection of SEB. Background technique [0002] Biotoxin contamination in food and the environment has the characteristics of a wide range of sources and a wide variety, posing a potential threat to human health, economic development and even social security and causing serious harm. Among them, Staphylococcus aureus enterotoxin B is easy to cause food poisoning events and has a very low lethal dose, and is also listed as a "potential biological warfare agent". Therefore, quantitative detection of SEB in food and environmental samples has received great attention from international organizations and regulatory authorities of various governm...

Claims

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Application Information

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IPC IPC(8): G01N21/25G01N21/47B22F1/00B22F9/24
CPCG01N21/25G01N21/255G01N21/474B22F9/24G01N2021/4742G01N2021/4769B22F1/054
Inventor 彭媛白家磊韩殿鹏宁保安李双秦康任舒悦李森高志贤
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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