Preparation method for preparing peptide molecules by applying enzymolysis technology
A technology using enzymes and technologies, applied in the field of preparing peptide molecules by using enzymolysis technology, can solve the problems of insufficient separation of enzymolysis products, low yield of peptide molecules, large molecular weight of peptides, etc. Enhanced enzymatic hydrolysis and small molecular weight peptides
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Embodiment 1
[0022] A method for preparing peptide molecules using enzymolysis technology, comprising the steps of:
[0023] S1. Select one or more of soybeans, black beans, red beans, mung beans, peas, wheat, rice, sorghum and oats to prepare vegetable protein powder;
[0024] S2. Preparation of protein solution: Dissolve protein powder in small molecule activated water, mix evenly at a ratio of 1:3, and prepare a protein solution; preheat the protein solution in a constant temperature water bath at 90°C for 10 minutes and then cool it to 40~ 50°C;
[0025] S2. Enzymatic hydrolysis of protein: adjust the cooled protein solution with 1mol / L sodium hydroxide to keep the pH value at 5.5-6.0, then add protease, vitamin B1, vitamin E and poria cocos, and put it in a water bath at 40-50°C Underwater hydrolysis for 18-24 hours, continuously add 1mol / L sodium hydroxide during the reaction process to keep the pH value of the solution constant, adjust the pH value to 7.0 after the hydrolysis is co...
Embodiment 2
[0030] A method for preparing peptide molecules using enzymolysis technology, comprising the steps of:
[0031] S1. Select one or more of soybeans, black beans, red beans, mung beans, peas, wheat, rice, sorghum and oats to prepare vegetable protein powder;
[0032] S2. Preparation of protein solution: dissolve protein powder in small molecule activated water, mix evenly at a ratio of 1:4, and prepare protein solution; preheat the protein solution in a constant temperature water bath at 90°C for 10 minutes and then cool it to 40~ 50°C;
[0033] S2. Enzymatic hydrolysis of protein: adjust the cooled protein solution with 1mol / L sodium hydroxide to keep the pH value at 6.0-6.5, then add protease, vitamin B1, vitamin E and poria cocos, and put it in a water bath at 40-50°C Underwater hydrolysis for 18-24 hours, continuously add 1mol / L sodium hydroxide during the reaction process to keep the pH value of the solution constant, adjust the pH value to 7.0 after the hydrolysis is comp...
Embodiment 3
[0038] A method for preparing peptide molecules using enzymolysis technology, comprising the steps of:
[0039] S1. Select one or more of pigs, ducks, fish, meat, eggs, cattle, and sheep to prepare animal protein powder.
[0040] S2. Preparation of protein solution: dissolve protein powder in small molecule activated water, mix evenly at a ratio of 1:5, and prepare protein solution; preheat the protein solution in a constant temperature water bath at 90°C for 15 minutes and then cool it to 40~ 50°C;
[0041] S2. Enzymatic hydrolysis of protein: adjust the cooled protein solution with 1mol / L sodium hydroxide to keep the pH value at 5.5-6.0, then add protease, vitamin B1, vitamin E and poria cocos, and put it in a water bath at 40-50°C Underwater hydrolysis for 18-24 hours, continuously add 1mol / L sodium hydroxide during the reaction process to keep the pH value of the solution constant, adjust the pH value to 7.0 after the hydrolysis is completed, and then use ultrasonic assis...
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