Photoelectrochemical aptamer sensor and preparation method and application thereof

An aptamer sensor and photoelectrochemical technology, applied in the field of biosensors, can solve the problems of poor accuracy and specificity, high detection cost, complicated operation, etc., and achieve regular and orderly apertures, high utilization of light energy, and improved separation efficiency. Effect

Active Publication Date: 2021-01-19
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the abuse of diazines has brought some adverse effects and caused serious pollution to the environment
Existing methods for measuring diazines include: high performance liquid chromatography, enzyme-linked immunosorbent assay, capillary electrophoresis, immunoassay and electrochemical methods, etc., but there are complex operations, poor accuracy and specificity in these methods, Problems such as high detection cost

Method used

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  • Photoelectrochemical aptamer sensor and preparation method and application thereof
  • Photoelectrochemical aptamer sensor and preparation method and application thereof
  • Photoelectrochemical aptamer sensor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A photoelectrochemical aptamer sensor of the present invention, comprising a conductive glass electrode used as a working electrode in a three-electrode system, the reaction end surface of the conductive glass electrode is modified with composite nanomaterials, and the composite nanomaterials are loaded graphite modified by gold nanoparticles Composed of thibrid hexagonal boron nitride nanosheets of carbon nitride, aptamer probes are self-assembled on the surface of composite nanomaterials, aptamer probes can specifically recognize and capture pollutant molecules, gold nanoparticles modified The hexagonal hexagonal boron nitride nanosheet loaded with graphite phase is based on the hexagonal boron nitride nanosheet as the carrier, and the graphitic carbon nitride is grown in situ on the surface of the hexagonal boron nitride nanosheet. Prepared by modifying gold nanoparticles on the surface of hexagonal boron nitride nanosheets loaded with graphitic carbon nitride.

[00...

Embodiment 2

[0079] An application of the photoelectrochemical aptasensor of the present invention in the detection of organophosphorus pesticides. The photoelectrochemical aptasensor constructed based on functional nanomaterials prepared in Example 1 is used to detect environmental pollutants. The application of the photoelectrochemical aptasensor prepared by the thibrid hexagonal boron nitride nanosheets loaded with graphitic carbon nitride modified by nanoparticles in the detection of diazines comprises the following steps:

[0080] (1) Add the diazine solution dropwise on the reaction end surface of the tin dioxide conductive glass electrode of the photoelectrochemical aptasensor prepared in Example 1, and incubate at 60° C. for 1 h to make the photoelectrochemical aptasensor Aptamer probes specifically recognize and capture diazines.

[0081] (2) With the tin dioxide conductive glass electrode capturing diazine in the step (1) as the working electrode, the saturated calomel electrode ...

Embodiment 3

[0089] Investigate the detection accuracy of the gold nanoparticle-loaded graphitic carbon nitride-loaded hexagonal boron nitride nanosheets as functional nanomaterials for the detection of environmental pollutants, specifically to investigate the gold-nanoparticle-loaded graphite Detection Accuracy of Photoelectrochemical Aptasensors Prepared from Thhied Hexagonal Boron Nitride Nanosheets of Phase Carbon Nitride.

[0090] In order to further verify the detection effect of the photoelectrochemical aptasensor prepared in Example 1 in practical applications, the photoelectrochemical aptasensor was used for target detection in actual samples (assay method with reference to Example 2), and the recovery rate experiment.

[0091] (1) The photoelectrochemical aptasensor prepared in Example 1 was used to detect the concentration of diazine in Xiangjiang water, tap water and apple supernatant respectively. The specific steps are: after pretreatment of different solutions such as filtra...

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Abstract

The invention discloses a photoelectrochemical aptamer sensor and a preparation method and application thereof. The photoelectrochemical aptamer sensor comprises a conductive glass electrode, the surface of the reaction end of the conductive glass electrode is modified with a composite nano material, and an aptamer probe is self-assembled on the surface of the composite nano material; and the composite nano material is a gold nanoparticle modified sulfur hybrid hexagonal boron nitride nanosheet loaded with graphite phase carbon nitride. The preparation method comprises the step of sequentiallyloading the gold nanoparticle-modified graphite-phase carbon nitride-loaded sulfur hybrid hexagonal boron nitride nanosheet and the aptamer probe on the surface of the reaction end of the conductiveglass electrode. The sensor has the advantages of high stability, long service life, strong anti-interference capability, wide detection range, low detection limit and the like, the preparation methodis simple in process, safe, pollution-free and low in cost, and the sensor can be widely used for detecting pollutants in media such as water bodies and organisms, and has the advantages of high utilization rate, wide application range, high application value and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a photoelectrochemical aptamer sensor and its preparation method and application, in particular to a sulfurized hexagonal boron nitride nanosheet loaded with graphite phase carbon nitride modified by gold nanoparticles The photoelectrochemical aptasensor and its preparation method and application. Background technique [0002] Due to the advantages of simple instrumentation, low cost, rapid analysis, and high sensitivity, photoelectrochemical sensors have attracted increasing research interest. In the photoelectrochemical sensor detection system, the working electrode is used to convert light energy into an electrical signal that is linearly related to the concentration, so the working electrode plays an important role in the photoelectrochemical sensor detection system. Various photoactive semiconductor materials such as TiO 2 , ZnO, CdS, and CdSe, etc., can effectively conve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327B82Y15/00B82Y30/00
CPCB82Y15/00B82Y30/00G01N27/3276G01N27/3278
Inventor 汤琳谭集穗彭博欧阳细莲朱旭冯程洋
Owner HUNAN UNIV
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