Preparation method and application of coated yeast fermentation product
A technology of yeast fermentation and yeast extract, which is applied in the field of preparation of film-coated yeast fermentation, can solve the problems of unsatisfactory treatment effect and easy drug or hormone dependence, so as to improve skin and mucous membrane immunity, prevent wound infection, The effect of preventing moisture evaporation
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Embodiment 1
[0045] A preparation method of coated yeast fermentation product, comprising the following steps:
[0046] Step 1: Prepare medium
[0047] 1) YPD medium for primary seed medium
[0048] YPD medium: Dissolve 10g of coated yeast extract, 20g of YNB, and 20g of glucose in 1000mL of deionized water, autoclave at 115°C, and set aside;
[0049] 2) BSM medium for fermentation expression medium
[0050] BSM medium: Dissolve the following substances in 1L deionized water in the following order: 26.7ml85% phosphoric acid 85%, 18.2g potassium sulfate, 14.9g magnesium sulfate heptahydrate, 0.93g calcium sulfate dihydrate, 4.13g potassium hydroxide; Use 25% ammonia water to adjust the pH to 5.5, add 0.2% YNB;
[0051] Step 2: Fermentation Expression
[0052] 1) Take the positive colony with identified activity, inoculate it into a baffle bottle of sterilized 200mL YPD medium, and cultivate it with shaking at 29°C and 200rpm for 24 hours, so that the absorbance value at 600nm wavelength...
Embodiment 2
[0076] A preparation method of coated yeast fermentation product, comprising the following steps:
[0077] Step 1: Prepare medium
[0078] 1) YPD medium for primary seed medium
[0079] YPD medium: Dissolve 10g of coated yeast extract, 20g of YNB, and 20g of glucose in 1000mL of deionized water, autoclave at 115°C, and set aside;
[0080] 2) BSM medium for fermentation expression medium
[0081] BSM medium: Dissolve the following substances in 1L deionized water in the following order: 26.7ml85% phosphoric acid 85%, 18.2g potassium sulfate, 14.9g magnesium sulfate heptahydrate, 0.93g calcium sulfate dihydrate, 4.13g potassium hydroxide; Use 25% ammonia water to adjust the pH to 5.5, add 0.2% YNB;
[0082] Step 2: Fermentation Expression
[0083] 1) Take the positive colony with identified activity, inoculate it in a baffle bottle of sterilized 200mL YPD medium, and cultivate it with shaking at 29°C and 200rpm for 24 hours, so that the absorbance value at 600nm wavelength r...
Embodiment 3
[0107] A preparation method of coated yeast fermentation product, comprising the following steps:
[0108] Step 1: Prepare medium
[0109] 1) YPD medium for primary seed medium
[0110] YPD medium: dissolve 10g of coated yeast extract, 20g of YNB, and 20g of glucose in 1000mL of deionized water, autoclave at 115°C, and set aside;
[0111] 2) BSM medium for fermentation expression medium
[0112] BSM medium: Dissolve the following substances in 1L deionized water in the following order: 26.7ml85% phosphoric acid 85%, 18.2g potassium sulfate, 14.9g magnesium sulfate heptahydrate, 0.93g calcium sulfate dihydrate, 4.13g potassium hydroxide; Use 25% ammonia water to adjust the pH to 5.5, add 0.2% YNB;
[0113] Step 2: Fermentation Expression
[0114] 1) Take the positive colony with identified activity, inoculate it in a baffle bottle of sterilized 200mL YPD medium, and cultivate it with shaking at 29°C and 200rpm for 24 hours, so that the absorbance value at 600nm wavelength r...
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