Preparation method and application of coated yeast fermentation product

A technology of yeast fermentation and yeast extract, which is applied in the field of preparation of film-coated yeast fermentation, can solve the problems of unsatisfactory treatment effect and easy drug or hormone dependence, so as to improve skin and mucous membrane immunity, prevent wound infection, The effect of preventing moisture evaporation

Inactive Publication Date: 2021-02-12
吉林省蓝浦浩业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the functional components of the existing functional facial masks for treating acne and dermatitis are drugs and hormones, the therapeutic effect is not ideal, and a large amount of long-term use is likely to cause drug or hormone dependence, and a kind of film-coated yeast is provided. Fermented product, the fermented product is fermented with YNB instead of peptone, the product produced by using it has antibacterial effect, and has the functions of moisturizing, promoting wound healing, reducing scar, perfect function, can treat hormone-dependent dermatitis, and does not contain preservatives , can be used for a long time, safe and reliable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of coated yeast fermentation product, comprising the following steps:

[0046] Step 1: Prepare medium

[0047] 1) YPD medium for primary seed medium

[0048] YPD medium: Dissolve 10g of coated yeast extract, 20g of YNB, and 20g of glucose in 1000mL of deionized water, autoclave at 115°C, and set aside;

[0049] 2) BSM medium for fermentation expression medium

[0050] BSM medium: Dissolve the following substances in 1L deionized water in the following order: 26.7ml85% phosphoric acid 85%, 18.2g potassium sulfate, 14.9g magnesium sulfate heptahydrate, 0.93g calcium sulfate dihydrate, 4.13g potassium hydroxide; Use 25% ammonia water to adjust the pH to 5.5, add 0.2% YNB;

[0051] Step 2: Fermentation Expression

[0052] 1) Take the positive colony with identified activity, inoculate it into a baffle bottle of sterilized 200mL YPD medium, and cultivate it with shaking at 29°C and 200rpm for 24 hours, so that the absorbance value at 600nm wavelength...

Embodiment 2

[0076] A preparation method of coated yeast fermentation product, comprising the following steps:

[0077] Step 1: Prepare medium

[0078] 1) YPD medium for primary seed medium

[0079] YPD medium: Dissolve 10g of coated yeast extract, 20g of YNB, and 20g of glucose in 1000mL of deionized water, autoclave at 115°C, and set aside;

[0080] 2) BSM medium for fermentation expression medium

[0081] BSM medium: Dissolve the following substances in 1L deionized water in the following order: 26.7ml85% phosphoric acid 85%, 18.2g potassium sulfate, 14.9g magnesium sulfate heptahydrate, 0.93g calcium sulfate dihydrate, 4.13g potassium hydroxide; Use 25% ammonia water to adjust the pH to 5.5, add 0.2% YNB;

[0082] Step 2: Fermentation Expression

[0083] 1) Take the positive colony with identified activity, inoculate it in a baffle bottle of sterilized 200mL YPD medium, and cultivate it with shaking at 29°C and 200rpm for 24 hours, so that the absorbance value at 600nm wavelength r...

Embodiment 3

[0107] A preparation method of coated yeast fermentation product, comprising the following steps:

[0108] Step 1: Prepare medium

[0109] 1) YPD medium for primary seed medium

[0110] YPD medium: dissolve 10g of coated yeast extract, 20g of YNB, and 20g of glucose in 1000mL of deionized water, autoclave at 115°C, and set aside;

[0111] 2) BSM medium for fermentation expression medium

[0112] BSM medium: Dissolve the following substances in 1L deionized water in the following order: 26.7ml85% phosphoric acid 85%, 18.2g potassium sulfate, 14.9g magnesium sulfate heptahydrate, 0.93g calcium sulfate dihydrate, 4.13g potassium hydroxide; Use 25% ammonia water to adjust the pH to 5.5, add 0.2% YNB;

[0113] Step 2: Fermentation Expression

[0114] 1) Take the positive colony with identified activity, inoculate it in a baffle bottle of sterilized 200mL YPD medium, and cultivate it with shaking at 29°C and 200rpm for 24 hours, so that the absorbance value at 600nm wavelength r...

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PUM

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Abstract

The invention provides a preparation method and application of a coated yeast fermentation product, and provides a preparation method of a coated yeast fermentation product. The method comprises the following steps: 1, preparing a culture medium: using a YPD culture medium as a primary seed culture medium, wherein the fermentation expression medium is a BSM medium. 2, performing fermentation expression. The invention also discloses application of the coated yeast fermentation product. The coated yeast fermentation product is a natural fermentation yeast product, and is rich in vitamins, essential amino acids, enzymes and yeast peptides. The coated yeast fermentation product filtrate can increase the expression of sericin and prevent the reduction of serine protein tyrosine mediated sericin, and the coated yeast fermentation product filtrate can increase the expression of caspase-14 in epidermal keratinocytes in a 3D model. The product can resist bacteria and diminish inflammation, promote wound healing, repair skin barriers and improve skin and mucous membrane immunity.

Description

technical field [0001] The invention relates to the field of production and manufacture of skin care products, in particular to a preparation method and application of a fermented product of coated yeast. Background technique [0002] After the mask or dressing is applied to the face, it can temporarily isolate the skin from the outside world, and the substance in the mask will wrap the skin tightly, blocking the skin from the outside air, and on the one hand, allowing water to slowly penetrate into the epidermis The stratum corneum, let the cells of the stratum corneum "drink enough water" in a humid environment, so that the deep cells can absorb enough water, so that the skin will become soft and increase elasticity; at the same time, the surface of the skin will also warm up, promoting capillary The blood vessels expand, which is beneficial to remove the external dust, chemical pollutants and microorganisms contaminated in the pores, and also helps to eliminate the waste ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9728A61Q19/00A61Q17/00A61P17/00A61P17/10A61P17/02
CPCA61K2800/85A61Q17/00A61Q19/00A61K8/9728A61P17/00A61P17/02A61P17/10
Inventor 眭春曹原眭新王启名曹珊周兰夏菲
Owner 吉林省蓝浦浩业科技有限公司
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