Preparation method of composite preparation and application of composite preparation in preparation of medicine for preventing or treating sleep disorder
A compound preparation and uniform mixing technology, applied in the field of medicine, can solve the problems of sleep disorder rebound, tolerance and residual effect, drug dependence of patients, etc., and achieve the effects of improving sleep, shortening the latency to fall asleep, and improving sleep conditions.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0061] Embodiment 1 A method for extracting compound preparations from Ashitaba, lotus seeds, soybeans, hawthorn and capsicum, comprising the steps of:
[0062] (1) Preparation of Ashitaba extract
[0063] Take 5kg of dry stems and leaves of Ashitaba and grind them into 80-mesh powder, put them into an extraction tank, add 25L of 50% (v / v) ethanol aqueous solution to it each time, and reflux extraction at 60°C for 2 times, each time for 1 hour , combined the 2 extracts, concentrated under reduced pressure, and vacuum freeze-dried to obtain 677.2g of Ashitina extract powder. The content of chlorogenic acid in this extract powder was measured to be 3.2wt%, and it was set aside.
[0064] (2) Preparation of lotus seed extract
[0065] Get 5 kg of shelled dry lotus seeds and grind them into 80 mesh powders, put them into an extraction tank, add 25 L of 50% (v / v) ethanol aqueous solution therein each time, and reflux extraction at 60 ° C for 2 times, each time for 1 hour, Combine ...
Embodiment 2
[0074] Embodiment 2 A method for extracting a composite preparation from Ashitaba, lotus seeds, soybean, hawthorn and pepper, comprising the steps of:
[0075] (1) Preparation of Ashitaba extract
[0076] Take 5kg of dry stems and leaves of Ashitaba and grind them into 200-mesh powder, put them into an extraction tank, add 50L of 80% (v / v) ethanol aqueous solution to it each time, and reflux extraction at 80°C for 2 times, each time for 2 hours , combined the 2 extracts, concentrated under reduced pressure, and vacuum freeze-dried to obtain 1201.3g of Ashitina extract powder. The content of chlorogenic acid in this extract powder was measured to be 3.7wt%, and it was set aside.
[0077] (2) Preparation of lotus seed extract
[0078] Get 5 kg of shelled dry lotus seeds and grind them into 200 mesh powder, put them into an extraction tank, add 50 L of 80% (v / v) aqueous ethanol solution therein at a time, and reflux extraction at 60°C for 2 times, each for 2 hours, Combine the ...
Embodiment 3
[0087] The influence of embodiment 3 compound preparations on mouse direct sleep
[0088] The test animals were 120 SPF-grade Kunming male (8-week-old) mice provided by Hubei Experimental Animal Center (provided by Liaoning Changsheng Biotechnology Co., Ltd.). Drink water and eat freely, keep the room temperature at 25±1°C, and the light-dark cycle is 12 hours. Pre-feed for a week.
[0089] 1. Grouping and administration
[0090] The 120 mice were divided into three groups: the first group was the pentobarbital sodium sleep time extension group, the second group was the pentobarbital sodium subthreshold dose hypnosis group, and the third group was the pentobarbital sodium Sleep latency group; each group has four subgroups: blank control group, low, medium and high dose groups, with 10 mice in each group. The blank control group was given the same amount of distilled water by oral gavage, and the low, middle and high dose groups of the compound preparation were given the cor...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More