Lactobacillus casei for improving silage quality of paper mulberry and application of lactobacillus casei

A technology of Lactobacillus casei and paper mulberry, which is applied in the field of Lactobacillus casei, can solve the problems of not being able to improve the effect of paper mulberry silage, the effect of paper mulberry silage is not good, and the number of lactic acid bacteria is small, so as to improve the quality of mulberry silage and increase the nutritional value And palatability, low cost effect

Active Publication Date: 2021-02-12
博益德(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are fluff distributed on both sides of the leaves of the mulberry tree, which is easy to absorb miscellaneous bacteria in the air, the number of lactic acid bacteria attached to the raw materials is small, and the buffer energy is high, which leads to poor silage effect of the mulberry tree.
In order to solve the problem of silage failure due to the lack of lactic acid bacteria and many miscellaneous bacteria in the raw material of mulberry, it is necessary to add additional lactic acid bacteria agents. However, the addition of conventional commercial lactic acid bacteria cannot improve the effect of mulberry silage, and there is no Lactobacillus casei additive specially used for mulberry silage. See the report

Method used

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  • Lactobacillus casei for improving silage quality of paper mulberry and application of lactobacillus casei
  • Lactobacillus casei for improving silage quality of paper mulberry and application of lactobacillus casei
  • Lactobacillus casei for improving silage quality of paper mulberry and application of lactobacillus casei

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Experimental program
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Effect test

Embodiment 1

[0023] The screening of embodiment 1 mulberry silage special strain

[0024] 1) Liquid enrichment: select a mulberry silage sample with a pH of 4.0-4.5, take 3-5g of the sample and put it into 100mL of sterilized MRS liquid medium, and culture it statically in a 37°C incubator for 36h-48h. Among them, the MRS liquid medium is composed of 2% sucrose, 1% peptone, 1% beef extract, 0.5% yeast extract powder, 0.5% sodium acetate, 0.2% ammonium citrate, 0.2% K 2 HPO 4 , 0.05% MgSO 4 ·7H 2 O, 0.02% MnSO 4 4H 2 O, 0.1% Tween, and the balance are composed of water, and the pH is adjusted to 6.2-6.4.

[0025] 2) Solid culture: draw 1mL of the above-mentioned liquid-enriched bacterial solution and carry out 10 days with normal saline. -4 、10 -5 、10 -6 Gradient dilution, draw 200 μL of the diluted solution and evenly spread it on the MRS solid medium, culture it statically in a 37°C incubator for 36h-48h, pick the colony with good shape and growth, and further streak and purify it...

Embodiment 2

[0039] The probiotic property of embodiment 2 Lactobacillus casei of the present invention

[0040] Inoculate the isolated Lactobacillus casei CGMCC NO.20432 (BFEC-7), Lactobacillus plantarum of CGMCC NO.13132, and Enterococcus faecalis of CGMCC NO.16062 in MRS liquid medium, and culture them statically in a 37°C incubator , from the fourth hour, use a microplate reader to detect the absorbance every hour, and record the absorbance value to evaluate the growth rate of the strain. For experimental data, see image 3 . Among them, Lactobacillus plantarum numbered CGMCC NO.13132 and Enterococcus faecalis numbered CGMCC No.16062 have been described in "A General Composite Fermentation Agent for Livestock and Poultry and Its Preparation and Application" with application number 201911203375.7. I won't go into details here. The bacterial strains selected in this application are three bacterial strains finally selected and determined through a large number of experimental verificat...

Embodiment 3

[0053] The silage experiment of embodiment 3 mulberry silage special bacterial agent

[0054] The culture fluid of bacterial strain CGMCC NO.13132, NO.20432, NO.16062 (wherein the bacterium concentration in CGMCC NO.13132 and NO.20432 bacterial fluid is respectively 10 8 CFU / mL, the bacterial concentration in NO.16062 bacterial solution is 10 7 CFU / mL) at a volume ratio of 0.5:2:0.5 to make a compound silage agent, cut the fresh mulberry branches and leaves into 1-2cm lengths, and add (mL / g) to the fresh mulberry branches and leaves Add compound silage agent for 1%, that is, add compound silage agent 1.0mL in 100g of fresh mulberry branches and leaves, and add molasses of 2%-5% of silage branches and leaves quality. After mixing well, put it in a breathing bag for compaction and sealing, and silage at 25°C-35°C for 40-50 days, and take silage on the 0th, 5th, 10th, 20th, 30th, and 40th day of the silage experiment, and use a pH meter to detect The pH value was determined by ...

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Abstract

The invention provides lactobacillus casei for improving the silage quality of paper mulberry, the lactobacillus casei is preserved in China General Microbiological Culture Collection Center on August17, 2020, and the preservation number is CGMCC NO.20432. The mixed microbial inoculum of the lactobacillus casei and bacterial strains CGMCC NO.13132 and CGMCC NO.16062 is used for carrying out ensiling fermentation on paper mulberry to prepare the silage. The lactobacillus casei provided by the invention has high synergy, inhibits harmful bacteria, generates a large amount of lactic acid, and rapidly reduces the pH value of a fermented material. The strain is compounded and combined with two strains of CGMCC NO.13132 and CGMCC NO.16062 to be used in paper mulberry silage, lactic acid can berapidly generated, the pH value of the paper mulberry silage is rapidly reduced, growth and propagation of harmful microorganisms are inhibited, the paper mulberry silage quality is improved, the nutritional value and palatability of the feed are improved, application is easy, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of microbial fermented feed, and in particular relates to Lactobacillus casei for improving the quality of mulberry silage and an application thereof. Background technique [0002] The shortage of protein feed resources and high-quality roughage resources is the main factor restricting the development of my country's animal husbandry, and the development of new forage resources is an important solution to the development of my country's animal husbandry. Paper mulberry is a deciduous tree of the Moraceae family unique to my country. It has the characteristics of fast growth, strong stress resistance, drought resistance, salt and alkali resistance, etc. The crude protein content of the whole plant can reach 20%, and the protein content of leaves is alfalfa protein. 1.4 times of content. However, mulberry accumulates a lot in the rainy season, and its water content is as high as 70%, which is not convenient fo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18C12R1/245
CPCC12N1/20A23K30/18C12R2001/245C12N1/205A23V2400/125A23V2400/169
Inventor 陈达刘世杰刘晶晶傅杰田久东
Owner 博益德(北京)生物科技有限公司
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