Photoelectrochemical aptasensor based on nanozyme signal amplification and its preparation method and application

An aptamer sensor and photoelectrochemical technology, applied in the field of biosensors, can solve problems such as poor accuracy and specificity, threat to human health, and complex instruments and equipment, and achieve high specificity, excellent photoelectric conversion efficiency, and good dispersion performance. Effect

Active Publication Date: 2022-03-11
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the application of CAP is currently limited
However, due to illegal use, CAP residues are now found in rivers and even animal-derived foods such as honey, eggs, and milk, posing a serious threat to human health
The current methods for measuring CAP include: high performance liquid chromatography, enzyme-linked immunosorbent assay, capillary electrophoresis, and electrochemical methods, etc., but these methods have complex equipment, cumbersome operations, poor accuracy and specificity, and detection high cost issues

Method used

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  • Photoelectrochemical aptasensor based on nanozyme signal amplification and its preparation method and application
  • Photoelectrochemical aptasensor based on nanozyme signal amplification and its preparation method and application
  • Photoelectrochemical aptasensor based on nanozyme signal amplification and its preparation method and application

Examples

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Effect test

Embodiment 1

[0058] A photoelectrochemical aptasensor based on nanozyme signal amplification of the present invention, comprising a conductive glass electrode used as a working electrode in a three-electrode system, and the reaction end surface of the conductive glass electrode is modified with benzene ring-doped carbon nitride nanosheets , the benzene ring doped carbon nitride nanosheet surface is covalently cross-linked with a specific double-stranded DNA recognition probe, the double-stranded DNA recognition probe is combined with a PtNi nanozyme signal probe, and the PtNi nanozyme signal probe is mainly composed of PtNi nano The wire is hybridized with streptavidin (it can also be considered to be mainly composed of PtNi nanowires hybridized with streptavidin). Among them, the specific double-stranded DNA recognition probe is used to identify and capture the pollutants to be tested, and it can also specifically capture the PtNi nanozyme signal probe, which is used to induce the formatio...

Embodiment 2

[0087] An application of the photoelectrochemical aptamer sensor based on nanozyme signal amplification of the present invention in the detection of antibiotics. In this example, the photoelectrochemical aptamer sensor based on nanozyme signal amplification prepared in Example 1 is used, and the antibiotic is specifically For chloramphenicol, the nucleic acid aptamer in the photoelectrochemical aptasensor has specific recognition and capture functions for chloramphenicol molecules.

[0088] The application process is as follows:

[0089] (a) Add different concentrations of chloramphenicol solution dropwise to the reaction end surface of the conductive glass electrode in the photoelectrochemical aptasensor based on nanozyme signal amplification, and react for 40min to make the specificity on the photoelectrochemical aptamer sensor The aptamer specifically recognizes and captures the chloramphenicol molecule, and then washes the modified electrode with Tris-Hcl buffer solution. ...

Embodiment 3

[0100] Evaluate the detection accuracy of the photoelectrochemical aptamer sensor based on nanozyme signal amplification in Example 1, and use the photoelectrochemical aptamer sensor for target detection in actual samples using the standard addition method (refer to Example 2 for the assay method) , for recovery experiments.

[0101] (1) The photoelectrochemical aptamer sensor prepared in Example 1 was used to detect the concentration of chloramphenicol in Xiangjiang water and milk respectively. The specific steps are: after pretreatment of different samples such as filtration, take the supernatant and use Tris-Hcl The buffer solution was adjusted to pH 7.4. The concentration of the target substance in the sample (containing chloramphenicol) is with reference to Table 1, and finally the photoelectrochemical aptasensor of Example 1 is used to detect chloramphenicol in the solution to be tested according to the method of Example 2, and the measurement results are listed in Table...

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Abstract

The invention discloses a photoelectrochemical aptamer sensor based on nanozyme signal amplification, a preparation method and application thereof. The sensor includes a conductive glass electrode used as a working electrode in a three-electrode system. The surface of the conductive glass electrode is modified with benzene ring-doped carbon nitride nanosheets, and the surface of the benzene ring-doped carbon nitride nanosheets is covalently cross-linked. The double-stranded DNA recognition probe is combined with a PtNi nanozyme signal probe, and the PtNi nanozyme signal probe is mainly composed of a PtNi nanowire. The preparation method comprises sequentially modifying the benzene ring-doped carbon nitride nanosheet, the double-stranded DNA recognition probe and the PtNi nanozyme signal probe on the surface of the conductive glass electrode reaction end. The sensor of the invention has high selectivity, repeatability and stability, and the preparation method is simple in process, low in cost and high in efficiency. The sensor can be used for detecting antibiotics, has wide detection range and low detection limit.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a photoelectrochemical aptamer sensor and its preparation method and application, in particular to a photoelectrochemical aptamer sensor based on nanozyme signal amplification and its preparation method and application. Background technique [0002] Due to the advantages of simple instrumentation, low cost, fast response, and high sensitivity, photoelectrochemical aptasensors have attracted increasing research interest. To improve the sensitivity of photoelectrochemical aptasensors, signal amplification is essential. Among various strategies, enzyme-mediated catalytic deposition is a simple and effective method, which can induce insoluble and non-conductive products on the electrode surface, hinder interfacial electron transfer, and then affect the change of photocurrent. However, the high cost of separation and purification of natural enzymes (such as horseradish peroxidase), ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N21/76
CPCG01N27/3276G01N27/3278G01N21/763
Inventor 汤琳朱旭彭博欧阳细莲谭集穗余江芳
Owner HUNAN UNIV
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