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Application of TIMP-1 in preparation of medicine for preventing or treating traumatic brain injury

A traumatic, brain-damaging technique with medical applications that can address issues such as disrupting the integrity of the blood-brain barrier

Pending Publication Date: 2021-03-09
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Altered expression of TJ-associated proteins has been found to disrupt the integrity of the blood-brain barrier in multiple TBI models

Method used

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  • Application of TIMP-1 in preparation of medicine for preventing or treating traumatic brain injury
  • Application of TIMP-1 in preparation of medicine for preventing or treating traumatic brain injury
  • Application of TIMP-1 in preparation of medicine for preventing or treating traumatic brain injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. The protective effect of TIMP-1 on traumatic brain injury mice

[0038] 1. Experimental animals

[0039] SPF-grade male C57BL / 6J mice 22-25 g, male, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0040] 2. TBI model making

[0041] After the mice were anesthetized with isoflurane, the scalp was incised at 0.8 mm posterior to the right coronal suture and 1.3 mm lateral to the midline, and a bone hole with a diameter of 3 mm was drilled. Using the improved Feeney free fall injury device, a 20g heavy hammer was used to freely fall from 25cm to impact the ram, and the blow depth was 3mm, and the scalp was sutured. In the sham operation group, the scalp was incised and drilled, and then the scalp was sutured without hammering.

[0042] 3. Animal grouping and administration:

[0043] Mice were divided into sham operation group, model group, TIMP-1 low-dose group (30 μg / kg), TIMP-1 medium-dose group (90 μg / kg), TIMP-1 high-d...

Embodiment 2

[0055] Example 2. The loss of TIMP-1 can lead to damage to the tight junctions between brain microvascular endothelial cells and HBMEC cells, and damage the integrity of the blood-brain barrier

[0056] 1. Cell Permeability Experiment

[0057] HBMEC cells (control group, knockout TIMP-1 group) were inoculated in 0.4 μm Transwell chambers coated with rat tail collagen I, and the chambers were placed in 24-well plates. After administration, FITC-Dextran with a molecular weight of 40Kd was added To the upper chamber, the final concentration was 1 mg / ml. After 2 hours, the culture medium in the well below the chamber was collected, and the leakage of FITC-Dextran was detected by a fluorescent microplate reader.

[0058] 2. Determination of transmembrane cell electrical resistance (TEER)

[0059] Inoculate HBMECs in a 0.4 μm Transwell chamber coated with rat tail collagen I, place the chamber in a 12-well plate, and insert the long end of the Millicell-ERS electrode into the lowe...

Embodiment 3

[0069] Example 3.Protective effect of TIMP-1 on blood-brain barrier damage in isolated traumatic brain injury model

[0070] 1. Isolated traumatic brain injury model

[0071] Establish an in vitro model of traumatic brain injury using a hypoxic chamber: add IL-1β to the HBMEC cell culture medium to a final concentration of 20 ng / ml, put the cell culture dishes of each group into the stemcell hypoxic chamber, and place a tube containing 10 ml of sterile water at the bottom of the chamber. In a large Petri dish, 95% nitrogen and 5% CO are continuously fed into the hypoxic chamber for 10 minutes 2 The gas was mixed to ensure that the cells were completely placed in a hypoxic environment, and then the chamber was placed in a 37°C incubator for 24h.

[0072] 2. Statistical analysis of data

[0073]Data are expressed as mean ± standard error (mean ± SEM), statistical analysis using one-way analysis of variance, " # ” means compared with the control group, where ## P* ” means com...

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Abstract

The invention discloses application of TIMP-1 in preparation of a medicine for preventing or treating traumatic brain injury. The invention discloses application of TIMP-1 in preparation of a medicinefor treating blood-brain barrier dysfunction of traumatic brain injury. The medicine can be in the form of injection powder, water aqua, lipidosome and nano materials. The protein TIMP-1 can prolongthe incubation period of a mouse with cerebral trauma in a rotarod test; the motion balance capability of the mouse with cerebral trauma on a balance beam is improved; and the Evans blue permeabilityof brain tissues is reduced. In brain microvascular endothelial cells HBMEC, the TIMP-1 can participate in maintaining intercellular tight junction integrity, up-regulating tight junction complex expression, reducing luciferase leakage and increasing intercellular resistance. The invention also provides application of the protein TIMP-1 as a CD63-Integrin[beta]1 ligand in preparation of a medicinefor treating central nervous system diseases caused by blood-brain barrier dysfunction. The TIMP-1 plays a role by combining with a cell membrane complex CD63-Integrin[beta]1 to regulate and controlthe assembly of important molecules F-actin of cytoskeleton so as to maintain the intercellular tight junction integrity. The protein TIMP-1 has a good application prospect in treating the blood-brainbarrier dysfunction of thetraumatic brain injury.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of endogenous inhibitor of matrix metalloproteinase-1 (Tissue inhibitor metalloproteinases-1, TIMP-1) in the preparation of drugs for preventing or treating traumatic brain injury. Background technique [0002] Traumatic brain injury (Traumaitc brain injury, TBI) is a disease that causes severe damage to brain tissue due to various traumas. It is one of the common emergencies in the field of neurosurgery. bit reason. At present, the main clinical treatment strategies are surgical debridement, hematoma removal, and mannitol to reduce intracranial pressure, but there is no FDA-approved drug for this disease. The difficulty in the treatment of traumatic brain injury is not due to the first injury to the body, but the dysfunction of the blood-brain barrier after the first injury. A large number of inflammatory factors and immune cells in the peripheral blood enter the brain t...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K38/17A61P25/00
CPCA61K48/005A61K38/1709A61P25/00
Inventor 彭英唐婧姝康钰莹黄龙舰
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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