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High-conversion-rate xylitol strain and preparation method thereof

A high conversion rate, xylitol technology, applied in the biological field, can solve the problems of high cost of culture medium and increased production cost, and achieve the effects of simple and easy method, environmental protection and high conversion rate

Inactive Publication Date: 2021-03-09
SHANDONG TAIYANGZHIYE +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The biotransformation method also has some disadvantages. For example, additives such as nickel and sodium gluconate need to be added during the fermentation process, and the cost of the medium during the expanded cultivation process is high, resulting in an increase in production costs.

Method used

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  • High-conversion-rate xylitol strain and preparation method thereof

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Embodiment 1

[0033] The screening of embodiment 1 bacterial strain

[0034] In the fermentation section of the xylose workshop, chromatographic detection found that the content of xylitol in the fermented liquid in a fermentation tank was more than 3 times higher than the usual detection, and samples were taken immediately for separation and cultivation. The xylose medium formula: xylose 10% by mass, heptahydrate 0.02 mass % of magnesium sulfate, 0.05 mass % of yeast extract, (NH 4 )H 2 PO 4 0.05% by mass, KH 2 PO 4 0.02% by mass, pH 5.8, if making a plate culture medium, add 2% by mass of agar (sterilized by an automatic pressure steam sterilizer at 120° C. for 20 minutes).

[0035] The fermenter is sampled, inoculated into xylose medium at a ratio of 5% by mass, cultivated in a 180rpm shaking incubator at 30°C for 12 hours, taking the culture solution and marking it on the xylose medium plate, and cultivating in a biochemical incubator at 30°C for 3- On the 4th day, high-quality c...

Embodiment 2

[0037] Embodiment 2 Fermentation experiment

[0038] Strain test tube culture: take out the preferred yeast slant from the refrigerator at 2-4°C, pick a single colony and inoculate it in a test tube containing 5ml of seed medium (5% wort medium, sterilized at 120°C for 15 minutes), Incubate in a biochemical incubator at 30°C for 24h (shake the test tube for 2-3 minutes every 4-8h).

[0039] Seed bottle culture: according to 10% inoculum, transfer the test tube seed solution to a 150ml Erlenmeyer flask containing 45ml seed medium (5% wort medium, sterilized at 120°C for 20 minutes) and cultivate for 12h (the culture condition is 30 °C, 180rpm).

[0040] 1000ml seed bottle cultivation: by 10% inoculum size, transfer the test tube seed solution to 450ml seed culture medium (components are 3 mass % of xylose, 1 mass % of glucose, 0.7 mass % of corn steep liquor powder, magnesium sulfate heptahydrate 0.02% by mass, adjust the pH value to 5.8, defoamer 0.005% by mass, sterilize at...

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Abstract

The invention relates to a preparation method of a high-conversion-rate xylitol strain. The preparation method comprises: (1) in a xylose fermentation workshop, detecting fermentation broth, screeningthe fermentation broth with a high xylitol content, and fermenting the fermentation broth by using saccharomyces cerevisiae; (2) performing sampling from a fermentation tank of the fermentation brothwith the highest xylitol content, inoculating a xylose culture medium with the fermentation broth, selecting bacterial colonies, outperforming enlarged culture on a xylose culture medium flat plate,and performing ultraviolet irradiation for mutagenesis; and (3) respectively performing enlarged culture on the mutated strains, inspecting fermentation products, and selecting the strain with the highest xylitol content and the least byproducts. The strain is preserved by the China General Microbiological Culture Collection Center, and the serial number of the strain is CGMCC No.20693.

Description

technical field [0001] The disclosure relates to the field of biotechnology, in particular to a strain of xylitol with high conversion rate and a preparation method thereof. Background technique [0002] Agricultural wastes such as straws, husks, and cores are rich in crude fibers (pentanose), but these agricultural wastes are mostly disposed of by waste or incineration, resulting in a great waste of resources and environmental pollution. And xylitol can be obtained from xylose by hydrolysis of crude fiber (pentose), and xylitol can be obtained by catalytic hydrogenation of xylose. Using agricultural wastes as raw materials to prepare xylitol is an effective way to turn agricultural and sideline products into treasures, protect the environment, and save resources. [0003] Xylitol is produced by reducing xylose. As a functional sweetener, it is an auxiliary therapeutic agent for diabetics. It has functions such as weight loss, intestinal regulation, and liver function impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N13/00C12N15/01C12N1/18C12P7/18C12R1/865
CPCC12N1/36C12N13/00C12N15/01C12P7/18
Inventor 仝庆文路庆辉曹衍军袁守彦刘泽华应广东张伟周天
Owner SHANDONG TAIYANGZHIYE