Adriamycin and indocyanine green co-loaded polysaccharide-based nanoparticles as well as preparation method and application of adriamycin and indocyanine green co-loaded polysaccharide-based nanoparticles
A technology of indocyanine green and nanoparticles, which can be applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc. Controllable release and other issues, to achieve simple purification process, improve tumor treatment effect, and good biocompatibility
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[0039] A method for preparing nanoparticles co-loaded with doxorubicin and indocyanine green in the present invention, first, doxorubicin and indocyanine green are respectively loaded on the formhylated hyaluronic acid and water-soluble On the chitosan, they are mixed in a certain proportion to form nanoparticles loaded with doxorubicin and indocyanine green, and then modified by layer-by-layer self-assembly process to form aldylated hyaluronic acid, water-soluble chitosan and Aldylation of hyaluronic acid with targeting ligands to yield polysaccharide-based nanoparticles co-loaded with doxorubicin and indocyanine green. Specifically include the following steps:
[0040] (1) Preparation of aldoxylated hyaluronic acid nanoparticles loaded with doxorubicin: using sodium periodate oxidation method to synthesize aldylated hyaluronic acid with an oxidation degree of 40% to 100% or 5% to 20%, wherein The formhylated hyaluronic acid with an oxidation degree of 40% to 100% is purifie...
Embodiment 1
[0059] (1) Preparation of aldylated hyaluronic acid nanoparticles loaded with doxorubicin: the sodium periodate oxidation method was used to synthesize aldylated hyaluronic acid (MW=8kDa) with a degree of oxidation of 40%, and the specific process was as follows: Add sodium periodate with a mass concentration of 1.0% hyaluronic acid aqueous solution to 0.4 times the molar amount of the hyaluronic acid structural unit, react in the dark for 8 hours at room temperature, then add 2 mL of ethylene glycol to terminate the reaction, and dialyze and purify in deionized water for 48 hours to obtain Formhylated hyaluronic acid with a theoretical oxidation degree of 40%;
[0060] Prepare this formhylated hyaluronic acid into a solution with a concentration of 6 mg / mL, and then add doxorubicin with a concentration of 2 mg / mL dropwise according to the mass ratio of doxorubicin to formylated hyaluronic acid at a ratio of 1:6. aqueous solution, reacted at room temperature for 0.5 hours, the...
Embodiment 2
[0076] (1) Preparation of aldylated hyaluronic acid nanoparticles loaded with doxorubicin: the sodium periodate oxidation method was used to synthesize aldylated hyaluronic acid (MW=8kDa) with a degree of oxidation of 40%, and the specific process was as follows: Add sodium periodate with a mass concentration of 1.0% hyaluronic acid aqueous solution to 0.4 times the molar number of hyaluronic acid repeating structural units, react in the dark for 24 hours at room temperature, then add 2 mL of ethylene glycol to terminate the reaction, and dialysis and purify in deionized water for 72 hours. The aldehydlated hyaluronic acid with a theoretical oxidation degree of 40% is obtained;
[0077] Prepare this formhylated hyaluronic acid into a solution with a concentration of 6 mg / mL, and then add doxorubicin with a concentration of 2 mg / mL dropwise according to the mass ratio of doxorubicin to formylated hyaluronic acid at a ratio of 1:5. aqueous solution, after reacting at room temper...
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