A kind of tissue adhesive and its preparation method and application

A tissue adhesive and cross-linking agent technology, used in applications, surgical adhesives, pharmaceutical formulations, etc., can solve the problem of inability to fully meet clinical needs, poor mechanical properties, and super-strong hydrogels lack of cell affinity and tissue. Adhesion and other issues, to achieve the effect of good wide applicability, good biocompatibility, and high adhesion strength

Active Publication Date: 2022-07-15
CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional hydrogels have poor mechanical properties due to their high water content; general super-strong hydrogels lack cell affinity and tissue adhesion, and cannot fully meet clinical needs.

Method used

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  • A kind of tissue adhesive and its preparation method and application
  • A kind of tissue adhesive and its preparation method and application
  • A kind of tissue adhesive and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 2 g of gelatin (bovine origin) was dissolved in 20 mL of deionized water at 60°C to obtain a 10 wt / vol% gelatin solution. After the pH value was adjusted to 8.5 with sodium hydroxide solution, 0.2 g / mL aqueous tannic acid solution was added dropwise to the gelatin solution using a syringe (a total of 1 mL of tannic acid aqueous solution was used), and after it was completely dissolved and uniformly mixed ( The time was controlled at 0.5h), and the pH of the solution was adjusted to 7.1 with hydrochloric acid solution, which was recorded as solution I.

[0064] 0.04 g of genipin was dissolved in 1 mL of 70% ethanol solution at room temperature. Subsequently, the solution I and the genipin solution (volume ratio of 1:1) were poured into a double-barrel syringe, extruded, and subjected to in-situ cross-linking reaction at room temperature (25° C.) for 10 min to obtain high-strength adhesion. Strong tissue adhesive.

[0065] Use a double-barrel syringe to extrude solution...

Embodiment 2

[0069] 1.5 g of gelatin (fish source) was dissolved in 20 mL of deionized water at 40°C to give a 7.5 wt / vol % gelatin solution. After adjusting the pH value to 8.1 with sodium hydroxide solution, use a syringe to add 0.5 g / mL gallic acid aqueous solution dropwise to the gelatin solution (a total of 1 mL of gallic acid aqueous solution is used), and after it is completely dissolved and mixed evenly (time control) At 0.75h), the pH of the solution was adjusted to 7.1 with a hydrochloric acid solution, which was designated as solution I.

[0070] 0.08 g of genipin was dissolved in 2 mL of 75% ethanol solution at room temperature. Subsequently, solution I and genipin solution (volume ratio of 3:1) were poured into a double-barrel syringe, extruded, and subjected to in-situ cross-linking reaction at room temperature (20° C.) for 15 min to obtain high-strength adhesion. Strong tissue adhesive.

[0071] Use a double-barrel syringe to extrude solution I and genipin solution (volume...

Embodiment 3

[0075] 1.5 g of gelatin (porcine source) was dissolved in 30 mL of deionized water at 40° C. to obtain a 5 wt / vol % gelatin solution. After the pH value was adjusted to 8.8 with sodium hydroxide solution, 0.8 g / mL tannic acid aqueous solution was added dropwise to the gelatin solution using a syringe (a total of 1 mL of tannic acid aqueous solution was used), and the pH of the solution was adjusted to 7.1 with hydrochloric acid solution , denoted as solution I.

[0076] 0.1 g of genipin was dissolved in 1 mL of 75% ethanol solution at room temperature. Subsequently, solution I and genipin solution (volume ratio of 1.5:1) were poured into a double-barrel syringe, extruded, and subjected to in-situ cross-linking reaction at room temperature (30° C.) for 20 min to obtain high-strength adhesion. Strong tissue adhesive.

[0077] Use a double-barrel syringe to extrude solution I and genipin solution (volume ratio of 1.5:1) to the surface of pigskin, rabbit heart, aminated glass, e...

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Abstract

The present application discloses a tissue adhesive and its preparation method and application. A tissue adhesive, which is on the surface of pigskin, when the surface adhesion time is less than 8h, the adhesion strength is ≤55kPa; when the surface adhesion time is ≥8h, the adhesion strength is >55kPa. Because the tissue adhesive contains amino groups, phenolic hydroxyl groups and natural cross-linking agents, it can form non-covalent and covalent bonds with groups such as amino groups and sulfhydryl groups on the surface of the tissue. Strong adhesion strength. The preparation method of the tissue adhesive has the advantages of simple process, low cost of raw materials, and all materials of natural origin. The tissue adhesive has good biocompatibility, high adhesive strength under physiological conditions, is easy and safe to use, and has a good application prospect in tissue adhesive repair.

Description

technical field [0001] The application belongs to the field of hydrogel preparation, and in particular relates to a tissue adhesive with high-strength adhesion properties, a preparation method and application thereof. Background technique [0002] Every year millions of patients around the world need sutures due to trauma or surgery. Currently, sutures and rivets are used clinically to suture wounds. Although this method is firmly sutured to make the wound less prone to cracking, its wide application is limited due to inconvenient operation, easy infection, scarring after healing, affecting the appearance and increasing the pain of patients. Therefore, a tissue adhesive with high adhesion properties was developed. Very necessary. Adhesive hydrogels with high water content and tissue-like structure are one of the most important biomaterials for use as surgical sealants and wound dressings. They show superior wound closure and tissue regeneration properties compared to curr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/104A61L24/0031A61L24/001A61L2400/06
Inventor 徐婷王荣张永和
Owner CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI
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