Cell immobilization preserving fluid, preparation method and application thereof

Abstract

The invention provides a cell immobilization preservation solution, a preparation method and application thereof. The cell immobilization preservation solution is prepared from the following components in percentage by weight: 1-5% of phosphate, 30-70% of fatty alcohol or polyol, 5-10% of organic amine salt, 0.5-1% of antibiotics and 15-60% of water. According to the invention, the cell immobilization preservation solution provided by the invention still keeps cell activity under the condition of not containing aldehyde compounds, is used for preserving and immobilizing cells, avoids the situation that antigen protein cannot be effectively recognized by antibodies due to the fact that protein structures are damaged by combination of amino acids and aldehyde substances in the cells, and does not need to use high-temperature and high-pressure equipment for antigen repair before an immunochemical staining experiment so as to simplify the experiment steps and avoid the problems of high experiment risk and unstable result caused by the use of high-temperature and high-pressure instruments and equipment, so that the whole chemical staining experiment is simpler, more convenient, more stable and safer.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to a cell fixation preservation solution and a preparation method and application thereof. Background technique [0002] For the immunohistochemical process of liquid-based exfoliated cells, the cells need to be placed in cell fixation and storage solution. Immunohistochemistry is a commonly used detection method in clinical diagnosis and histopathology. Antigen retrieval cannot be ignored in the process of immunohistochemistry. key step. In the process of immunohistochemistry, the commonly used fixative contains formalin. The main component of formalin is formaldehyde, which can be used to preserve cell shape, stabilize the cell matrix, and prevent cells from autophagy or protein translocation. Formaldehyde in formalin can specifically bind to certain amino acid residues, such as lysine, tyrosine, histidine, arginine, glutamine, asparagine, etc. In this reaction, the ac...

AI Technical Summary

Problems solved by technology

Because this process consumes the hydrogen in the amino group, it disrupts the original hydrogen bonds and interactions between amino acids, destroying the tertiary or quaternary structure of the protein, resulting in the antigen not being well recognized by the antibody, thus affecting Antibody recognition effect of immune cells

For this reason, immunocytochemical staining using formalin-containing cell fixatives to fix and fix the preservation solution generally requires the use of high temperature, high pressure or chemical reagents for antigen retrieval, which will lead to complicated and cumbersome immunocytochemical staining experimental steps and inconsistent results. Stable and experimental risk

Images