Application of composition to preparation of medicine for treating gastric cancer based on regulation and control of cancer suppression related genes

A technology of composition and application, which is applied in the field of preparing gastric cancer drugs based on the regulation of tumor suppressor-related genes, and can solve the problems of poor prognosis and the like

Pending Publication Date: 2021-04-30
HANDAN PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CDK1 (cyclin-dependent kinase 1) is an important regulator of G2-M phase transition. It has been reported that CDK1 can cooperate with cyclin B to promote cells to enter meiosis, and the expression of CDK1 is upregulated in CRC circulating tumor cells , CDK1 plays a key role in promoting the G2-M transition, regulating the G1 process, and the transition from G1 to S phase. Studies have shown that the increase in the expression level of CDK1 is closely related to the proliferation of cancer cells or the poor prognosis of cancer, and the high expression rate of CDK1 indicates that Patients with CRC have a poorer prognosis

Method used

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  • Application of composition to preparation of medicine for treating gastric cancer based on regulation and control of cancer suppression related genes
  • Application of composition to preparation of medicine for treating gastric cancer based on regulation and control of cancer suppression related genes
  • Application of composition to preparation of medicine for treating gastric cancer based on regulation and control of cancer suppression related genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Moluotan is composed of lily, Poria cocos, Scrophulariaceae, black medicine, Alisma, Ophiopogon japonicus, Angelica, Atractylodes macrocephala, capillary root, white peony root, dendrobium, calamus chinensis, Chuanxiong, notoginseng, Burnet, Corydalis, Puhuang, chicken The pure Chinese medicine preparation composed of 18 medicinal materials such as Neijin has the functions of harmonizing the stomach and invigorating the spleen, dredging collaterals and relieving pain, strengthening the spleen and reducing swelling, and can significantly reduce the expression of EGF and EGFR in patients with CAG, and the actual clinical application effect is quite good. Moluodan was selected as the treatment for chronic gastritis and chronic atrophy by the "Consensus Opinions on Chronic Gastritis in China" (2017) formulated by the Digestive Disease Branch of the Chinese Medical Association, and the "Consensus Opinions on the Diagnosis and Treatment of Chronic Gastritis by TCM Experts" (201...

Embodiment 2

[0058] Example 2 Identification of substances with tumor suppressor function of Molotan.

[0059] In this example, among the genes with significant changes in expression levels in the transcriptome analysis data, the changes in the transcripts of genes such as P53, TGF-β, CDK1, and CDK6 were used as marker genes for regulating tumor suppressors.

[0060] SGC-7901 cell line (preserved by our laboratory and purchased from ATCC cell bank) is used as the object of drug treatment. This cell has the characteristics of rapid reproduction and easy culture. The cultured SGC-7901 cells were seeded on a 6-well plate (50w cells were cultured in each well), and cultured in DMEM (10% FBS) medium until all adhered. The molodan candidate active components screened above were dissolved in DMSO, and applied to the SGC-7901 cell culture medium respectively, and the treated cells were collected after incubation for 24 hours. RNA was extracted according to the processing steps in the aforemention...

Embodiment 3

[0064] In this embodiment, apigenin, atractylodes lactone III, chrysophanol, ellagic acid, quercetin, limonin, cattail glycoside, pachymic acid, γ-terpinene, β-elemene and pomelo The 11 substances of corticosteroids were combined according to different molar ratios, and the advantages of promoting cell apoptosis under different combinations were verified.

[0065] SGC-7901 cell line (preserved by our laboratory and purchased from ATCC cell bank) is used as the object of drug treatment. This cell has the characteristics of rapid reproduction and easy culture.

[0066] Inoculate the cultured SGC-7901 cells on a 6-well plate (50w cells per well), culture them in DMEM (10% FBS) medium until they are all attached to the wall, and dissolve the 11 effective components screened above in DMSO , mix according to the following four combinations (molar concentration ratio):

[0067] Group A: Apigenin: Atractylodes lactone III: Chrysophanol: Ellagic acid: Quercetin = 4:10:1:6:8;

[0068]...

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Abstract

The invention belongs to the technical field of pharmaceutical preparations, particularly relates to an application of a composition to preparation of a medicine for treating gastric cancer based on regulation and control of cancer suppression related genes, and more particularly relates to application of the medicine for treating gastric cancer based on regulation and control of CDK1, CDK6, P53 and / or TGF-beta cancer suppression genes. On the basis of fingerprint spectrum analysis of a Chinese patent medicine Morhodamine, a plurality of medicine components in Morhodamine and target genes capable of being regulated and controlled by the medicine components are preliminarily determined; cancer inhibition related genes such as CDK1, CDK6, P53, TGF-beta and the like are selected as target genes for direct or indirect regulation and control of the morhodamine, and effective substances with a gastric cancer inhibition function in the morhodamine are verified. effective components including apigenin, atractylenolide III, chrysophanol, ellagic acid, quercetin, limonin, typhagoside, pachymic acid, gamma-terpinene, beta-elemene or naringenin are screened out, and theoretical support is provided for development of new drugs for treating gastric cancer.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and specifically relates to the use of a composition for the preparation of a drug for treating gastric cancer based on regulation of tumor suppressor-related genes, and more specifically relates to therapy based on regulation of CDK1, CDK6, P53 and / or TGF-β tumor suppressor genes Use of gastric cancer drugs. Background technique [0002] According to reports, the annual incidence of gastric cancer (GC) ranks 4th and 5th in the world, and the annual mortality rate ranks 2nd among all tumors. Although the diagnosis and treatment of gastric cancer have made great progress in recent years, its 5-year survival rate is still very low. As a highly malignant tumor, GC has the characteristics of unlimited cell proliferation, invasion, and metastasis, and it is a tumor with poor prognosis. It is of great significance to pay attention to and pay attention to the treatment and research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/122A61K31/366A61K31/365A61K31/7048A61K31/585A61K31/575A61K31/015A61K36/8984A61P35/00A61P1/00A61K35/57
CPCA61K31/352A61K31/122A61K31/366A61K31/365A61K31/7048A61K31/585A61K31/575A61K31/015A61K36/8967A61K36/076A61K36/808A61K36/54A61K36/884A61K36/8968A61K36/232A61K36/284A61K36/282A61K36/71A61K36/65A61K36/8984A61K36/236A61K36/258A61K36/739A61K36/66A61K36/88A61K35/57A61P35/00A61P1/00A61K2300/00
Inventor 陈致慜殷苗苗王珍刘会云王梦蕾
Owner HANDAN PHARMA
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