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Enzyme-based composite catalyst as well as preparation method and use method thereof

A composite catalyst and catalyst technology, applied in biochemical equipment and methods, enzymes, peptidases, etc., can solve the problems of product separation, increase in purification procedures, limit industrial expansion, and reduce by-products, etc., and achieve excellent chemical stability. Time and economic cost, the effect of low production cost

Pending Publication Date: 2021-04-30
广东省汇林包装科技集团有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former catalyst can be recycled and reused, which is helpful for industrial scale promotion. However, this method has the problem of high energy consumption. The catalyst needs to be activated under high temperature and pressure, and the catalytic activity is highly dependent on the surface structure of the material. , requiring fine control of structural parameters (such as pore size, surface area, functional groups, etc.), so this type of catalyst is rarely reported for industrialization
CN107382718A discloses a method for CaO / MCF mesoporous basic molecular sieve to catalyze the alcoholysis of polylactic acid: the catalyst reacts with polylactic acid at a temperature of 100°C to 140°C for 3 to 4 hours to obtain methyl lactate, the quality of methanol and polylactic acid The ratio reached 3:1. The harsh catalytic environment and high methanol consumption of this process will increase the difficulty of subsequent product separation and purification processes.
Biological enzyme is another effective catalyst to catalyze the degradation of polylactic acid plastics. It can usually be carried out under normal temperature and pressure, with few by-products, high activity and high selectivity; but the industrialization of biological enzymes first needs to solve two important problems: stability and recyclability, it is these two serious defects that limit its industrial expansion in plastic degradation and even environmental protection as a whole

Method used

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  • Enzyme-based composite catalyst as well as preparation method and use method thereof
  • Enzyme-based composite catalyst as well as preparation method and use method thereof
  • Enzyme-based composite catalyst as well as preparation method and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A preparation method for an enzyme-based composite catalyst, comprising the steps of:

[0034] (S1), 10 molar parts of metal salt and 30 molar parts of organic ligand were dissolved in 100 parts by volume of 0.2 mol / L phosphate buffer solution, heated and condensed at 110°C under reflux and stirred for 60 minutes to obtain a coordination polymer;

[0035] (S2), at a temperature of 55°C, add 10 mole parts of esterase-lactate dehydrogenase blend to the coordination polymer, and continue stirring for 60 minutes to obtain a catalyst mixture;

[0036] (S3), centrifuging, washing, and vacuum-drying the catalyst mixture at 50° C. for 12 hours to obtain an enzyme-based composite catalyst with an enzyme load of 8 wt%.

[0037] The metal salt is ferric nitrate; the organic ligand is dimethylimidazole.

[0038]The esterase-lactate dehydrogenase blend is formed by mixing esterase and D-lactate dehydrogenase at a molar ratio of 6:3.

[0039] The esterase is pronase.

[0040] A me...

Embodiment 2

[0045] A preparation method for an enzyme-based composite catalyst, comprising the steps of:

[0046] (S1), 10 molar parts of metal salt and 10 molar parts of organic ligand were dissolved in 100 parts by volume of 0.15 mol / L phosphate buffer solution, heated, condensed and refluxed at 90° C. and stirred for 40 minutes to obtain a coordination polymer;

[0047] (S2), at a temperature of 50°C, add 1 mole part of esterase-lactate dehydrogenase blend to the coordination polymer, and continue stirring for 40 minutes to obtain a catalyst mixture;

[0048] (S3), centrifuging, washing, and vacuum drying the catalyst mixture at 40°C for 12 hours to obtain an enzyme-based composite catalyst with an enzyme loading of 5 wt%.

[0049] The metal salt is zinc nitrate; the organic ligand is ethylenediaminetetraacetic acid.

[0050] The esterase-lactate dehydrogenase blend is formed by mixing esterase and D-lactate dehydrogenase at a molar ratio of 5:2.

[0051] The esterase is boroprotease...

Embodiment 3

[0057] A preparation method for an enzyme-based composite catalyst, comprising the steps of:

[0058] (S1) Dissolving 10 mol parts of metal salt and 50 mol parts of organic ligand in 100 parts by volume of 0.25 mol / L phosphate buffer solution, heating, condensing and stirring under reflux at 120°C for 80 minutes to obtain a coordination polymer;

[0059] (S2), at a temperature of 60°C, add 20 mole parts of esterase-lactate dehydrogenase blend to the coordination polymer, and continue stirring for 80 minutes to obtain a catalyst mixture;

[0060] (S3), after centrifuging, washing, and vacuum drying the catalyst mixture at 60° C. for 12 hours, an enzyme-based composite catalyst with an enzyme load of 10 wt % is obtained.

[0061] The metal salt is calcium chloride; the organic ligand is sodium alkylbenzenesulfonate.

[0062] The esterase-lactate dehydrogenase blend is formed by mixing esterase and D-lactate dehydrogenase at a molar ratio of 8:5.

[0063] The esterase is K-prot...

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Abstract

The invention relates to the technical field of preparation of catalytic degradation materials, and in particular, relates to an enzyme-based composite catalyst as well as a preparation method and a use method thereof. The preparation method of the enzyme-based composite catalyst comprises the following steps: (S1) dissolving metal salt and an organic ligand into a phosphate buffer solution, and heating, condensing and refluxing under a stirring condition to obtain a coordination polymer; (S2) adding an esterase-lactic dehydrogenase blend into the coordination polymer, and continuously stirring to obtain a catalyst mixed solution; and (S3) centrifuging the catalyst mixed solution, washing, and carrying out vacuum drying to obtain the enzyme-based composite catalyst. The coordination polymer is used as a carrier, and the esterase-lactic dehydrogenase blend is anchored in situ, so that the high activity and high selectivity of the esterase-lactic dehydrogenase can be exerted, and the carrier endows the esterase lactic dehydrogenase blend with excellent chemical stability.

Description

technical field [0001] The invention relates to the technical field of preparing catalytic degradation materials, in particular to an enzyme-based composite catalyst, its preparation method and its use method. Background technique [0002] Plastic is a great invention of mankind. It exhibits excellent plasticity, durability and chemical stability, and is widely used in industrial production and daily life. However, the problem of white pollution and dependence on non-renewable resources (such as petroleum) are also becoming more and more prominent, which urgently requires us to optimize and improve its process route so as to take into account the two development goals of energy saving and environmental protection. In recent years, starch from renewable plant resources (such as corn and straw) has been extracted as a raw material, and further saccharified and polycondensed to obtain a green, economical, and degradable plastic precursor-polylactic acid. With the "sustainable ...

Claims

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Application Information

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IPC IPC(8): C12N11/096
CPCC12N11/096C12N9/0006C12N9/63C12N9/20C12N9/52C12N9/58C12Y301/01003C12Y101/01028C12Y304/22004C12Y304/21064Y02P20/584
Inventor 刘武邹洋吴生焘王宏青龚韬
Owner 广东省汇林包装科技集团有限公司