Preparation method and application of photoelectric aptamer sensor for detecting pcb72
The technology of an aptamer sensor and PCB72 is applied in the field of preparation of photoelectric aptamer sensors, which can solve the problems of low sensitivity, complicated operation, poor selectivity, etc., and achieve the effects of high sensitivity, promotion of separation, and improvement of conductivity.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] (1) Polish the pure titanium foil with different sizes of sandpaper and ultrasonically clean it. The cleaned titanium foil was etched with hydrochloric acid and water (2:1, V:V) for 10-20 min at 120°C, and immediately rinsed with secondary water. Then, at a potential of 60 V, with 0.3 wt% NH 4 F and 2vol%H 2 The ethylene glycol solution of O was the electrolyte, the Ti foil was the anode, and the platinum sheet was the cathode, and the anodization was carried out for 2 h. After anodization, the prepared samples were placed in a muffle furnace and calcined at 450 °C for 120 min at a heating rate of 2 °C / min to make the TiO 2 Crystallization of NTs. like figure 1 As shown in A, TiO 2 NTs were grown upright on the surface of the titanium foil in situ, showing a distinct tubular structure with a diameter of 100 nm and a length of 6.3 μm.
[0038] (2) by a simple hydrothermal method on TiO 2 Modified BiVO on NTs 4 NPs. BiVO 4 The synthesis method is to dissolve...
Embodiment 2
[0041] The preparation method of the photoelectrochemical aptamer sensor for detecting PCB72 comprises the following steps:
[0042] Before immobilizing the aptamer, the disulfide bond of the aptamer was first reduced using 1,4-dithiothreitol. The aptamer solution was diluted to 3.0 μmol / L with Tris-EDTA buffer solution. The treated aptamer solution was added dropwise to BiVO modified with Au NPs 4 NPs-TiO 2 NTs photoelectrodes and incubated in the refrigerator at 4 °C for more than 12 h. Then, the aptamer-modified photoelectrode was rinsed with Tris-HCl buffer solution to remove unbound aptamer. The PCB72 photoelectrochemical aptamer sensor was prepared and stored at 4 °C for use as follows figure 2 Shown are the I-t curves of the photoelectrochemical aptamer sensor of the present invention at different construction stages in 0.1 mol / L PBS, pH 7.4 electrolyte solution. It can be seen from the figure that TiO 2 The photocurrent density of NTs is small (curve a). BiVO...
Embodiment 3
[0044] Photoelectric analysis method of photoelectrochemical aptamer sensor for detecting PCB72
[0045] (1) Prepare standard solutions of PCB72 with different concentrations from 1ng / L to 500ng / L;
[0046] (2) with the described photoelectrochemical aptamer sensor for detecting PCB72 as working electrode, saturated calomel electrode as reference electrode, platinum sheet electrode as counter electrode and 0.1mol / L PBS, the electrolyte solution of pH 7.41 constitutes three. Electrode system; the fixed electrode of the photoelectrochemical aptamer sensor of PCB72 is 1cm×1cm.
[0047] (3) Add the prepared PCB72 standard solution of the first concentration to the three-electrode system, incubate at room temperature for 40 min; then, under the irradiation of visible light above 420 nm, apply a 0.0V bias voltage, and use I-t technology to measure the concentration of the PCB72 standard solution Corresponding photocurrent response; using the above method to measure the photocurrent...
PUM
| Property | Measurement | Unit |
|---|---|---|
| recovery rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More 
PatSnap Eureka turns technology decisions into work you can execute. Powered by our Innovation Knowledge Graph, it runs expert workflows across engineering, life sciences, materials and intellectual property. Get your review-ready output in minutes.
