Detection reagent and detection kit for detecting risperidone and/or 9-hydroxyrisperidone

Abstract

The invention relates to the technical field of immunological detection, and particularly discloses a detection reagent and a detection kit for detecting risperidone and / or 9-hydroxy risperidone. The detection reagent comprises an enzyme-labeled working solution, a magnetic particle working solution and an indicator solution; the enzyme-labeled working solution comprises a conjugate obtained from risperidone, a 9-hydroxy risperidone hapten and a marker; the magnetic particle working solution comprises a conjugate obtained by coupling an antibody and magnetic particles; the indicator solution contains an indicator for quantitatively detecting the concentration of the marker; and the structures of the risperidone hapten and the 9-hydroxy risperidone hapten are shown as a formula I, wherein the antibody is generated in response to a conjugate formed by the risperidone hapten, the 9-hydroxy risperidone hapten and carrier protein through adoption of a covalent coupling method. The detection kit comprises the detection reagent. According to the invention, an immunological detection method with strong specificity and high sensitivity to risperidone and 9hydroxy risperidone is established.

Description

technical field [0001] This application relates to the technical field of immunological detection, more specifically, it relates to a detection reagent and a detection kit for detecting risperidone and / or 9-hydroxy risperidone. Background technique [0002] Schizophrenia is a serious, disabling mental illness. As social competition intensifies, the pace of life accelerates, and family structures change, people are under increasing pressure, resulting in increasingly prominent mental health problems. Schizophrenia is the most common mental illness. [0003] Risperidone is a phenylpropisoxazole derivative with the chemical name 3-[2-[4-(6-fluoro-1,2-benzisoxazol-3-yl)-1-piperidinyl]ethyl Base]-6,7,8,9-tetrahydro-2-methyl-4H-pyrido[1,2-α]pyrimidin-4-one is a new type of psychotropic drug. Risperidone targets 5-hydroxytryptamine (5-HT2) receptors and dopamine (D2) receptors, blocks their respective neurotransmitter absorption, thereby effectively improving psychotic symptoms....

AI Technical Summary

Problems solved by technology

However, patients taking risperidone will also metabolize inactive metabolites 7-hydroxyrisperidone and N-dealkylated risperidone, and most patients will also take sedatives while taking risperidone , tricyclic antidepressants, selective pentamerin tryptamine reuptake inhibitors, etc., the above factors will affect the detection of risperidone and 9-hydroxyrisperidone

[0005] At present, radioimmunoassay and high-performance liquid chromatography (HPLC) are mostly used for blood concentration determination of risperidone and 9-hydroxyrisperidone. Liquid chromatography requires multiple repeated extractions, which has the disadvantages of cumbersome, complicated, time-consuming and labor-intensive operations, and low throughput, which is not conducive to clinical promotion

[0006] For example, the publication date of the application is July 1, 2015, and the patent document with the publication number CN104755479A discloses a hapten of risperidone and paliperidone, and the publication date of the application is June 24, 2015. The patent document No. CN104736565A discloses a risperidone hapten antibody and its application. The above two patent documents propose derivatization at the 9th hydroxyl position and the F atom position of the 9-hydroxy risperidone molecule, and the prepared antibody There is an obvious cross-reaction with the inactive metabolite 7-hydroxyrisperidone, and the detection reagent prepared by this antibody may cause the determination of the plasma concentration of risperidone and paliperidone in the blood sample to be too high

For example, the patent document with application publication date of August 21, 2018 and application publication number CN108431040A discloses the amino acid sequence of a risperidone antibody, but the antibody still has a serious crossover with the inactive metabolite 7-hydroxyrisperidone response, less specific

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