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Preparation method of cellulose silicon-based cascade double-ring resonant cavity chip for detecting PCA3

A double-ring resonance, cellulose technology, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems such as the inability to observe the change of the signal of the cascaded double-ring resonator cavity sensor, the inability to realize the selective detection of PCA3, and the small number of PCA3 gene probes. , to achieve the effect of safer and more environmentally friendly operation process, lower testing costs, and easy availability of raw materials

Inactive Publication Date: 2021-05-11
济南国科医工科技发展有限公司
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Problems solved by technology

However, since the cascaded double-ring resonator sensor will show the same spectral signal for substances with the same refractive index, the selective detection of PCA3 cannot be achieved.
If the method of directly reacting the PCA3 gene probe with the hydroxyl functional group on the surface of the resonator is used, the number of immobilized PCA3 gene probes will usually be too small, so that the cascaded double-ring resonator sensor will not be observed when detecting PCA3 Changes in signal, or very small changes in signal observed

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  • Preparation method of cellulose silicon-based cascade double-ring resonant cavity chip for detecting PCA3

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[0048] The cellulose used in the preparation of the cellulose film in the present invention is cellulose and cellulose derivatives such as cellulose, carboxylated cellulose, polymerized cellulose, hydroxypropylmethylcellulose, and hydroxyethylcellulose.

[0049] The prepared cellulose film may further include cellulose copolymers or blends doped with other nanoparticles.

[0050] The fixation of the PCA3 probe in the present invention is to fix the PCA3 probe on the surface of the cellulose film through the covalent reaction of the PCA3 probe and the cellulose.

[0051] The PCA3 probe of the present invention is designed using the 4 exons of PCA3 as a template.

[0052] The process of modifying the PCA3 probe on the surface of the sensing ring 6 of the present invention comprises the following steps:

[0053] ① Pretreatment of silicon-based cascaded double-ring resonators;

[0054] ② Modification of epoxy silane or amino silane on the surface of sensing ring 6;

[0055] ③ F...

Embodiment 1

[0066] Immerse the silicon-based cascaded double-ring resonant cavity in a piranha solution (98% sulfuric acid: 30% hydrogen peroxide in a ratio of 7:3) and treat it at 80°C for 30 minutes, clean it with ultrapure water, and then immerse it in ammonia water: hydrogen peroxide: water Treat in a solution with a volume ratio of 1:1:5 for 1 h, and wash with ultrapure water. A 2% 3-aminopropyltriethoxysilane solution in toluene was added dropwise to the sensing ring of the silicon-based cascaded double-ring resonator treated above for treatment for 1 h, followed by treatment at 120° C. for 30 min. Then, 5 ml of an aqueous solution containing 0.271 g of carboxymethyl cellulose was put into the sensing ring, and reacted at 90° C. for 15 h. Then the sensing ring was put into 5ml of methanol solution containing 100mg of succinic anhydride, and reacted at room temperature for 24h. Then transfer the sensing loop to the newly prepared EDC / NHS (0.04M:0.01M) solution and let it stand for 1...

Embodiment 2

[0068] Immerse the silicon-based cascaded double-ring resonant cavity in a piranha solution (98% sulfuric acid: 30% hydrogen peroxide in a ratio of 7:3) and treat it at 80°C for 30 minutes, clean it with ultrapure water, and then immerse it in ammonia water: hydrogen peroxide: water Treat in a solution with a volume ratio of 1:1:5 for 1 h, and wash with ultrapure water. The surface of the sensing ring of the above-mentioned liquid-treated silicon-based cascaded double-ring resonator was added dropwise with an anhydrous toluene solution containing 1% 3-(2,3-epoxypropoxy)propyltrimethoxysilane for 30 minutes, After drying at 110° C. for 1 h, the sensing ring was put into 5 ml of DMSO solution containing 0.01 g LiCl and 0.5 g cellulose, and reacted at 80° C. for 24 h. Next, the sensing ring was put into 9 ml of tetrahydrofuran solution containing 25 mg of succinic anhydride and reacted overnight at room temperature for 24 hours. Then transfer the sensing loop to the newly prepar...

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Abstract

The invention discloses a preparation method of a cellulose silicon-based cascade double-ring resonant cavity chip for detecting PCA3, and belongs to the technical field of cascade double-ring resonant cavity sensing. The surface of a sensing ring of the chip is sequentially modified with a silane coupling agent, a cellulose film and a PCA3 gene probe; and the cellulose silicon-based cascade double-ring resonant cavity chip with the functionalized PCA3 gene probe is prepared. The surface of the sensing ring is modified with the cellulose nano-film, so that the fixing amount of the PCA3 gene probe on the surface of the sensing ring is increased, the quantity of combining the PCA3 and the chip is increased by utilizing the specific combination performance of the PCA3 gene probe and PCA3, the response signal of the silicon-based cascade double-ring resonant cavity sensor to the PCA3 is greatly improved, and the unmarked, high-sensitivity and high-selectivity detection of the PCA3 is realized. The functional chip for detecting PCA3 reduces the detection cost, and is expected to be widely applied to diagnosis and treatment of prostatic cancer.

Description

technical field [0001] The invention relates to the technical field of cascaded double-ring resonant cavity sensing, in particular to a method for preparing a cellulose silicon-based cascaded double-ring resonant cavity chip for detecting PCA3. Background technique [0002] Prostate cancer is one of the common tumors that threaten men's health. The incidence of prostate cancer in my country has been on the rise in recent years. Prostate cancer is described as a "silent killer", and it is not easy to be detected in the early stage. About 2 / 3 of the cancer patients have advanced to the advanced stage when they are diagnosed, and the overall survival period and quality of life are seriously affected. Data show that 65% to 75% of prostate cancer patients have bone metastases, bone pain, pathological fractures, limb movement disorders, spinal cord compression, hypercalcemia and other bone-related events, and even paralysis of the lower limbs. At the same time, patients with adva...

Claims

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Application Information

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IPC IPC(8): G01N21/25
CPCG01N21/25
Inventor 李秋顺董文飞李力姜琛昱葛明锋常智敏从瑛哥宁珊珊
Owner 济南国科医工科技发展有限公司
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