A kind of oral flora polymorphism regulator
A polymorphism and regulator technology, applied in antifungal agents, antibacterial agents, plant/algae/fungus/moss components, etc., can solve the relative balance, poor taste and safety issues affecting oral microbial diversity Can not be ignored and other issues, to achieve the effect of excellent maintenance of oral ecological diversity, sweet and refreshing taste, safe and non-toxic process
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Embodiment 1
[0072] Embodiment 1: Screening of natural plant extracts
[0073] 1. Instruments and materials
[0074] 1.1 Instrument
[0075] HCB-900V vertical laminar flow clean bench (Qingdao Haier Special Electric Co., Ltd.); SW-CJ-JF ultra-clean bench (Suzhou purification plant); HPX-9052MBE electric heating constant temperature incubator (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory); BSD-TX345 desktop shaker (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory); infinite M200 PRO microplate reader (US TECAN company); EYELA N-1210B rotary evaporator (Shanghai Ailon Instrument Co., Ltd.); EYELA-1000S Circulating water vacuum pump (Tokyo Physical and Chemical Instrument Co., Ltd.); FA124 electronic balance (Tianjin Yinuo Scientific Instrument Co., Ltd.); LS-B 50L vertical pressure steam sterilizer (Shanghai Medical Nuclear Instrument Factory).
[0076] 1.2 Materials
[0077] Streptococcus salivarius (S.salivarius, ATCC13419) was purchased from Guangdong Micr...
Embodiment 2
[0098] Embodiment 2. extract extraction process and proportioning research
[0099] 1. Instruments and materials
[0100] 1.1 Instrument
[0101] HCB-900V vertical laminar flow clean bench (Qingdao Haier Special Electric Co., Ltd.); SW-CJ-JF ultra-clean bench (Suzhou purification plant); HPX-9052MBE electric heating constant temperature incubator (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory); BSD-TX345 desktop shaker (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory); infinite M200 PRO microplate reader (US TECAN company); FA124 electronic balance (Tianjin Yinuo Scientific Instrument Co., Ltd.); LS-B 50L vertical Pressure steam sterilizer (Shanghai Medical Nuclear Instrument Factory). Agilent1260 high performance liquid chromatography, Agilent20RBAX Eclipse Plus chromatographic column (250mm×4.6mm, 5μm), YP1001 electronic balance (Shanghai Qinghai Instrument Co., Ltd.), AX205 1 / 100,000 electronic balance (Switzerland METTLER TOLEDO company), Mi...
Embodiment 3
[0240] Embodiment 3. preparation active ingredient of the present invention
[0241] Take 15 g of Scutellaria baicalensis coarse powder, add 15 times the amount of purified water, reflux for extraction for 2 hours, centrifuge and filter until clarification, and concentrate the filtrate under reduced pressure at 60°C to a clear paste with a crude drug concentration of about 1g / mL
[0242] Take 15 g of eucommia leaves, add 20 times the amount of purified water, soak and extract at 60°C for 2 hours, centrifuge and filter until clarified, and concentrate the filtrate at 60°C under reduced pressure to a clear paste with a crude drug concentration of about 1 g / mL.
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