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Preparation method and application of implantable in-situ formed chitosan hydrogel

A technology of in-situ molding and chitosan, which can be applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., and can solve problems such as safety retention that limits the development of implantable materials

Active Publication Date: 2021-05-18
XI'AN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the safety and retention in the body of the current carrier greatly limit the development of implantable materials.

Method used

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  • Preparation method and application of implantable in-situ formed chitosan hydrogel
  • Preparation method and application of implantable in-situ formed chitosan hydrogel
  • Preparation method and application of implantable in-situ formed chitosan hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of preparation method of chitosan hydrogel that can be implanted in situ molding of the present invention, specifically implement according to the following steps:

[0050] Step 1, chitosan is dissolved in the acetic acid solution, obtains the acetic acid solution of chitosan;

[0051] The massfraction of acetic acid solution is 1%; The concentration of chitosan in acetic acid solution is 20mg / mL;

[0052] Step 2, using N-isopropylacrylamide (NIPAm) and 2-acrylamido-2-methylpropanesulfonic acid (AMPS) free radical polymerization to prepare polyanionic PNAM, specifically:

[0053] Dissolve the monomer NIPAm and the monomer AMPS in the mixed solvent, and then put the initiator under N 2 Protected, and added dropwise to the mixed solvent under constant temperature stirring at 60°C. After the dropwise addition, the sealed system was reacted for 24 hours, and the reaction liquid was transferred to a dialysis bag with a molecular weight cut-off of 3500Da for dialysis ...

Embodiment 2

[0062] A kind of preparation method of chitosan hydrogel that can be implanted in situ molding of the present invention, specifically implement according to the following steps:

[0063] Step 1, chitosan is dissolved in the acetic acid solution, obtains the acetic acid solution of chitosan;

[0064] The massfraction of acetic acid solution is 1%;

[0065] The concentration of chitosan in acetic acid solution is 15mg / mL; the viscosity of chitosan is medium viscosity chitosan;

[0066] Step 2, using N-isopropylacrylamide (NIPAm) and 2-acrylamido-2-methylpropanesulfonic acid (AMPS) free radical polymerization to prepare polyanionic PNAM, specifically:

[0067] Dissolve the monomer NIPAm and the monomer AMPS in the mixed solvent, and then put the initiator under N 2 Protected, and added dropwise to the mixed solvent under constant temperature stirring at 60°C. After the dropwise addition, the sealed system was reacted for 24 hours, and the reaction liquid was transferred to a di...

Embodiment 3

[0076] A kind of preparation method of chitosan hydrogel that can be implanted in situ molding of the present invention, specifically implement according to the following steps:

[0077] Step 1, chitosan is dissolved in the acetic acid solution, obtains the acetic acid solution of chitosan;

[0078] The massfraction of acetic acid solution is 1%;

[0079] The concentration of chitosan in acetic acid solution is 20mg / mL; chitosan is high viscosity chitosan (>400mPa.s);

[0080] Step 2, using N-isopropylacrylamide (NIPAm) and 2-acrylamido-2-methylpropanesulfonic acid (AMPS) free radical polymerization to prepare polyanionic PNAM, specifically:

[0081] Dissolve the monomer NIPAm and the monomer AMPS in the mixed solvent, and then put the initiator under N 2 Protected, and added dropwise to the mixed solvent under constant temperature stirring at 60°C. After the dropwise addition, the sealed system was reacted for 24 hours, and the reaction liquid was transferred to a dialysis ...

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Abstract

The invention discloses a preparation method of implantable in-situ formed chitosan hydrogel. The preparation method specifically comprises the following steps of: dissolving chitosan into an aceticacid solution to obtain an acetic acid solution of the chitosan; preparing polyanion PNAM by utilizing NIPAm and AMPS free radical polymerization; and under an ice-water bath condition, adding a PNAM aqueous solution and a NaHCO3 solution into the acetic acid solution of the chitosan, stirring for 30 minutes, then putting a sample into a constant-temperature water bath kettle at 37 DEG C, carrying out gelation, and finally carrying out freeze drying to obtain the chitosan hydrogel. The chitosan is taken as a carrier, and has the advantages of inhibiting and killing bacteria, diminishing inflammation and stopping bleeding, promoting wound healing and the like; in-situ gel prepared by a polyelectrolyte composite method not only has temperature sensitivity, but also has pH sensitivity, and can realize controlled release of medicines, so that the wound of a surgical operation is greatly reduced, and the operation cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical polymer materials, and in particular relates to a preparation method of chitosan hydrogel which can be implanted and formed in situ, and also relates to the application of the chitosan hydrogel. Background technique [0002] Cardiovascular disease is one of the major diseases that seriously threaten human life and health. Among them, the number of patients with myocardial infarction is huge and the mortality rate is high. The treatment of myocardial infarction mostly adopts coronary interventional therapy, that is, stent surgery or coronary artery bypass surgery, which is a major trauma operation, and it is easy to cause wound infection after surgery, resulting in secondary injury. [0003] Implantable in situ forming gel is a kind of tissue engineering scaffold, which is in the flow state of solution at room temperature and can wrap therapeutic drugs. When injected into the desi...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/32A61K9/06A61K31/616
CPCA61K47/36A61K47/32A61K9/06A61K9/0024A61K31/616
Inventor 赵婧王晓冉丁涛刘凯文邓春昱
Owner XI'AN POLYTECHNIC UNIVERSITY
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