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Saline-alkali tolerant phosphate-solubilizing marine bacterium 3-1a, microbial organic fertilizer, preparation method and application

A microbial organic fertilizer and saline-alkali-resistant technology, applied in the field of microbial organic fertilizer, can solve the problems of decreased soil fertility and soil microbial activity, and achieve the effects of promoting phosphorus absorption, reducing soil pH, and alleviating soil compaction problems

Active Publication Date: 2021-05-25
SHANDONG UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term frequent water-salt movement leads to a decline in soil fertility and soil microbial activity. It is difficult for conventional phosphorus-solubilizing bacteria to survive and function in such a high-saline environment.

Method used

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  • Saline-alkali tolerant phosphate-solubilizing marine bacterium 3-1a, microbial organic fertilizer, preparation method and application
  • Saline-alkali tolerant phosphate-solubilizing marine bacterium 3-1a, microbial organic fertilizer, preparation method and application
  • Saline-alkali tolerant phosphate-solubilizing marine bacterium 3-1a, microbial organic fertilizer, preparation method and application

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preparation example Construction

[0037] The third aspect of the present invention provides a preparation method of the above-mentioned microbial organic fertilizer, which specifically includes: pretreatment of the strain carrier, cultivation and inoculation of salt-alkali phosphate-tolerant marine bacteria 3-1a, and semi-solid fermentation of the microbial organic fertilizer.

[0038]In one or more embodiments of the present invention, the pretreatment of the strain carrier specifically includes air-drying, pulverizing, and sieving the edible fungus residue and corncob powder respectively, and sterilizing twice at 120° C. 30 minutes each time.

[0039] In one or more embodiments of the present invention, the sieving is as follows: passing the edible fungus residue through a 18-mesh sieve, passing the corn cob powder through a 18-mesh sieve and a 60-mesh sieve respectively, and using a ratio of 2:1:1 The mass ratio of the edible fungus slag passing through the 18 mesh sieve, the corn cob powder passing through...

Embodiment 1

[0054] Phosphorus-dissolving amount and medium pH change of salt-alkali tolerant phosphate-dissolving marine bacteria 3-1a within 5 days:

[0055] Firstly, the salt-alkali phosphate-hydrolysis-tolerant marine bacteria 3-1a was cultured in seawater LB medium for 24 hours (200 rpm, 25° C.).

[0056] Then, the bacterial solution was centrifuged at 4000rpm, washed twice with deionized water, resuspended with sterile water, inoculated to high-salt PVK medium with 5% inoculum (v / v), and cultured by sampling at intervals of 6h, 12h or 24h Based on pH, the supernatant was collected by centrifugation to measure the soluble phosphorus content in the supernatant, and the phosphorus-dissolving amount of the strain and the pH change curve of the medium were drawn within 5 days.

[0057] The formula of the above-mentioned high-salt PVK medium is: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, MgSO 4 0.5g / L, KCl0.3g / L, NaCl 33.3g / L, yeast powder 0.5g / L, FeSO 4 0.03g / L, MnSO 4 0.03g / L, Ca 3...

Embodiment 2

[0059] Determination of the phosphorus-solubilizing ability of salt-alkali tolerant phosphorus-solubilizing marine bacteria 3-1a under different salinity, pH, temperature and using different carbon and nitrogen sources:

[0060] High-salt PVK medium used: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, MgSO 4 0.5g / L, KCl 0.3g / L, NaCl 33.3g / L, yeast powder 0.5g / L, FeSO 4 0.03g / L, MnSO 4 0.03g / L, Ca 3 (PO 4 ) 2 5g / L, prepared with deionized water, adjusted the pH to 6.8-7.2 with 0.5M HCl and 0.5M NaOH, and sterilized by high-pressure steam at 115°C for 20min.

[0061] Wherein, the phosphorus-dissolving amount determination test of bacterial strain under different salinities adopts the following method, adjusts the content of sodium chloride in the above-mentioned salt-containing PVK medium, and is mixed with sodium chloride mass concentration and is 0.5%, 1%, 3%, 5%. %, 8%, 10% salt-containing PVK medium with six salinity gradients.

[0062] The phosphorus solubilization tes...

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Abstract

The invention relates to the field of microbial organic fertilizers, in particular to a saline-alkaline tolerant phosphate-solubilizing marine bacterium 3-1a, a microbial organic fertilizer and a preparation method and application thereof. The saline-alkaline tolerant phosphate-solubilizing marine bacteria 3-1a are separated from deep-sea hydrothermal port sediments, the saline-alkaline tolerant phosphate-solubilizing marine bacteria 3-1a can grow in a wide salinity, pH and temperature range and maintain high phosphate-solubilizing activity, and the microbial organic fertilizer prepared by taking the bacteria as an active material can effectively improve the problem of soil hardening, reduce the pH of soil increases the soil available phosphorus content, improves the soil fertility, promotes plant rhizosphere soil phosphorus conversion and phosphorus absorption, increases the plant biomass, reduces the use amount of chemical phosphate fertilizer, and reduces environmental pollution; in addition, the saline-alkaline tolerant phosphate-solubilizing marine bacterium 3-1a also has the function of reducing Cr (VI), can reduce 0-150mg / L of Cr (VI) under the condition of high salinity, and can be widely applied to treatment of chromium-containing soil and water.

Description

technical field [0001] The invention relates to the field of microbial organic fertilizers, in particular to a salt-alkali tolerant phosphorus-dissolving marine bacterium 3-1a, microbial organic fertilizers, preparation methods and applications. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Phosphorus is an essential mineral element for plant growth. It mainly participates in the synthesis of nucleic acid, phospholipid and ATP during plant growth, and promotes crop growth through photosynthesis, biological oxidation, nutrient absorption and cell catabolism. It determines the yield and quality of crops. quality. The form of phosphorus in soil that can be absorbed and ut...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/20C05G3/00C05G3/80C09K17/14C12R1/07C09K101/00C09K109/00
CPCC05F5/00C05F17/20C05G3/00C05G3/80C09K17/14C09K2101/00C09K2109/00C05F11/00C05F11/08Y02W30/40
Inventor 周维芝韩明月张梦汝刘喆陈秀兰
Owner SHANDONG UNIV
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