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Composite flora for preventing and controlling outbreak of maize seed fusarium and application of composite flora

A technology of compound flora and fusarium, applied in the direction of application, chemicals and bacteria for biological control, can solve environmental pollution, endanger human and animal health and other problems, and achieve the effect of reducing the outbreak rate and reducing the number

Active Publication Date: 2021-06-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Conventional chemical prevention and control measures will cause environmental pollution, endanger the health of humans and animals, etc.

Method used

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  • Composite flora for preventing and controlling outbreak of maize seed fusarium and application of composite flora
  • Composite flora for preventing and controlling outbreak of maize seed fusarium and application of composite flora
  • Composite flora for preventing and controlling outbreak of maize seed fusarium and application of composite flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Effects of the outbreak of Fusarium spp. on the growth of maize

[0049] The experiment consisted of two treatments: natural soil planting (NS) and sterilized soil planting (SS). 150g of natural soil and sterilized soil were placed in sterile tissue culture bottles, and 37.5ml of sterile water was added, stirred evenly, and kept in the dark. Cultivate for 1 week, and ensure that the soil moisture content is 20%-25%. The sterilized soil is sterilized by gamma rays, and the irradiation dose is >50kGray.

[0050] Transplant the corn seeds with the same growth potential that had been germinated in the sterile plate into tissue culture bottles, plant 5 corn plants in each culture bottle, and set up 6 replicates. The corn planting conditions for the pot experiment were 25°C, 16 hours of light and 8 hours of darkness, and a humidity of 50%.

[0051] After 8 days of corn growth, the incidence of corn was counted, and the plant height, fresh weight, chlorophyll SPAD...

Embodiment 2

[0056] Example 2: Isolation and identification of cultivable strains from the healthy corn rhizosphere

[0057] The planting conditions of potted corn are the same as in Example 1.

[0058] Healthy maize plants in natural soil were selected, and maize rhizosphere samples were collected on the 8th day after transplanting, and cultivable strains of maize rhizosphere were isolated by a combination of macroculture omics separation and plate coating.

[0059] Take the rhizosphere soil to prepare the soil microbial extract, and then carry out gradient dilution, select 10 -4 and 10 -5 Diluted microbial extracts were isolated in 96-well plates supplemented with 1:10 (v / v) tryptic soy broth. A microtiter plate with only 30%-40% of the wells with bacterial growth was selected to identify the 16S rRNA gene of the strain, and the genomic DNA was extracted from the bacterial liquid, and amplified by the polymerase chain reaction (PCR) technology using two-step barcode-labeled primers. A...

Embodiment 3

[0062] Example 3: Determination of core antagonistic bacteria in the complex flora

[0063] The 8 strains were streak cultured on solid TSB medium and placed in a constant temperature incubator at 30°C. After a single colony was formed, each strain was picked and cultured overnight in 3ml liquid TSB medium with shaking at 170rpm. Draw 2ml of bacterial solution into a sterile centrifuge tube, centrifuge at 8000rmp for 5 minutes, and then resuspend with PBS buffer to obtain the suspension of the tested strain.

[0064] Inoculate the Fusarium spp. on the center of the PDA plate and place it in a constant temperature incubator at 28°C. After the mycelia cover the plate, use a sterile puncher to take out the fungal block and transfer it to the center of a new PDA solid plate. Draw 10 μL of the suspension of the tested strains and inoculate them equidistantly on both sides of the fungal block, and place them in a constant temperature incubator at 30°C for 8 days.

[0065] results...

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Abstract

The invention discloses a composite flora for preventing and controlling the outbreak of maize seed fusarium and application of the composite flora. The compound flora is prepared from acinetobacter baumannii RFAci-1, pseudomonas stutzeri RFPse-1, pseudoxanthomonas japonicum RFPth-1, burkholderia cepacia RFBur-1, stenotrophomonas maltophilia RFSte-1, bacillus amyloliquefaciens RFBac-1, lysobacter soilis RFLys-1 and enterobacter ludwigii RFEnt-1. The composite flora is stable in structure, has the characteristics of efficient biocontrol and growth promotion, can significantly reduce the morbidity of fusarium diseases caused by seed fusarium in main maize planting varieties in China after being inoculated into soil, and can also promote the growth of corn plants.

Description

technical field [0001] The invention relates to a rhizosphere composite flora for preventing and controlling diseases caused by Fusarium maize seed-dwelling fungus and an application thereof, belonging to the technical field of crop soil-borne disease prevention and control. Background technique [0002] The seed-dwelling microorganisms inside the seeds constitute a very rich microbial community. They do not have a single mutualistic symbiotic relationship with plants, and there are also pathogenic pathogenic microorganisms in them. Most seeds carry pathogenic fungi inside, and the dominant genus is Fusarium, such as Fusarium oxysporum, Fusarium verticillium, Fusarium laminarum, Fusarium graminearum, etc. Fusarium spreads mainly through the vertical transmission between plant species into the interior of plant seeds, and then becomes the source of infection in the second year, causing various plant diseases. [0003] Fusarium species can produce toxins such as deoxynivaleno...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N63/27A01N63/20A01P21/00A01P3/00A01G22/20C12R1/38C12R1/07C12R1/01
CPCC12N1/20A01N63/22A01N63/27A01N63/20A01G22/20
Inventor 荀卫兵张瑞福刘梓豪任轶张楠徐志辉沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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