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Determination method of pyrrolizidine alkaloid in eupatorium fortunei

A technology of pyrrolizidine and pyrrolizidine in Perrin, applied in the field of determination of pyrrolizidine alkaloids in Perrin, can solve the problems of mutagenesis, teratogenicity, carcinogenicity, etc., and achieve accurate repeatability and detection results Accurate and ensure the effect of drug safety

Active Publication Date: 2021-06-08
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to liver toxicity, PAs are also toxic to the lung, heart, kidney, pancreas, brain, etc. and have obvious carcinogenic, mutagenic, and teratogenic effects; if taken for a long time, the medicinal material has a greater safety risk

Method used

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  • Determination method of pyrrolizidine alkaloid in eupatorium fortunei
  • Determination method of pyrrolizidine alkaloid in eupatorium fortunei
  • Determination method of pyrrolizidine alkaloid in eupatorium fortunei

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0038] The assay method of pyrrolizidine alkaloids in Perrin comprises the following steps:

[0039] Step 1, mixing the reference substance solution

[0040] Precisely weigh immedidine, sulfamethoxone, lindinaline, lindinaline, N-oxidized lindine, N-oxide lindine, N-oxide and N-oxide An appropriate amount of reference substance of amazone was formulated with 50% methanol water to prepare reference substance stock solutions with mass concentrations of 1, 1, 1, 5, 1, 10, 1 and 1 μg·mL-1 respectively; An appropriate amount of product stock solution was used to prepare mixed reference solution with mass concentrations of 20, 20, 20, 100, 20, 200, 20 and 20 ng·mL–1 with 50% methanol water.

[0041] Step 2, the test solution

[0042] Take 0.8g of the powder of different parts of Peilan medicinal material (passed through a No. 4 sieve), put it in a stoppered conical flask, add 0.05mol L –1 Sulfuric acid solution 40mL, ultrasonic (300W, 40kHz) extraction for 30min, filter, take the...

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Abstract

The invention relates to a determination method of pyrrolizidine alkaloid in eupatorium fortunei. The determination method comprises the following steps: (1) preparing a mixed reference substance solution containing intermedine, lycopsamine, rinderine, echinatine, N-oxidized lycopsamine, N-oxidized echinatine, N-oxidized imprint intermedine and N-oxidized lycopsamine; (2) putting the fortune eupatorium herb powder into a conical flask with a plug, adding 0.05 mol.L<-1> sulfuric acid solution, carrying out ultrasonic extraction and filtration, and taking a subsequent filtrate for purification; (3) determining, wherein the chromatographic column is a Waters ACQUITY UPLC HSS T3 chromatographic column; the column temperature being 40 DEG C; a mobile phase being an aqueous solution (A) containing 0.05% of formic acid and 2.5 mmol.L <-1> of ammonium formate and acetonitrile (B) containing 0.05% of formic acid and 2.5 mmol.L <-1> of ammonium formate, and the gradient elution conditions are as follows: 0-16 min, 0% B -> 4% B; 16-25 minutes, 4%B->20% B; the flow rate being 0.3 mL * min <-1>; the sample size being 1 [mu] L; a mass spectrum ion source being an electrospray ionization source, positive ions being adopted, a multi-reaction monitoring mode being adopted, the voltage of a capillary tube being 3.0 kV, the temperature of the ion source being 150 DEG C, and the solvent removal temperature being 500 DEG C. The method provides a scientific basis for safe medication of the eupatorium medicinal material.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to a method for determining pyrrolizidine alkaloids in Perrin. Background technique [0002] Peilan is the dry aerial part of Eupatorium fortunei Turcz., a plant of the genus Eupatorium fortunei Turcz. in the family Asteraceae. It is a species recorded in the 2020 edition of the "Chinese Pharmacopoeia". Treatment of diseases such as middle resistance, wrist swelling, nausea, sweet and greasy mouth, bad breath, excessive salivation, syndrome of heat-dampness, early onset of dampness and temperature, fever and fatigue, chest tightness and discomfort. Peilan is produced in Shandong, Jiangsu, Zhejiang, Jiangxi, Hubei, Hunan, Yunnan, Sichuan, Guizhou, Guangxi, Guangdong and Shaanxi. It is rare in the wild and cultivated by many. Wild ones live in roadside bushes and beside roadsides in ravines. The whole plant and flowers are fragrant, like la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/72G01N2030/047G01N2030/146
Inventor 昝珂左甜甜金红宇马双成王莹刘丽娜李耀磊王丹丹
Owner NAT INST FOR FOOD & DRUG CONTROL