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A kind of plant culture medium without explosive-producing compound and application thereof

A compound and medium technology, applied in the field of plant biology, can solve the problems of high production cost of mixed medium powder powder, unstable quality, cumbersome powder, etc., to promote plant growth and biomass accumulation, improve plant resistance, and reduce preparation costs Effect

Active Publication Date: 2022-07-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant culture medium of the present invention does not contain ammonium nitrate and potassium nitrate, which are easy to make explosives, and is convenient for plant tissue culture enterprises to prepare plant tissue culture medium formulas with low cost and good use effect, so as to fundamentally solve the problem of professional production by safety management departments. The management of ammonium nitrate and potassium nitrate, which are urgently needed by enterprises, is a cumbersome problem, as well as the high production cost and the high risk of unstable powder quality due to the high production cost of the production enterprise due to the need to purchase mixed medium powder.

Method used

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  • A kind of plant culture medium without explosive-producing compound and application thereof
  • A kind of plant culture medium without explosive-producing compound and application thereof
  • A kind of plant culture medium without explosive-producing compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Proliferation and culture of adventitious buds of Echinacea

[0037] (1) Obtaining aseptic adventitious bud material of Echinacea purpurea: Echinacea purpurea aseptic adventitious bud is obtained from young petiole through induction culture and regeneration. The height of adventitious bud is about 2cm, including terminal bud and 2-3 tender leaves.

[0038] (2) Preparation of proliferation medium: 30g / L of sucrose, 0.4mg / L of 6-BA and 0.01mg / L of NAA, 4.8g / L of agar, and 4.8g / L of agar were added to the formulations of NECC1, NECC2, NECC3 and MS medium respectively. The value is adjusted to 6.0.

[0039] (3) Culture method: The above-mentioned adventitious buds were inoculated (vertically inserted) into the above-prepared NECC1, NECC2, NECC3 and MS medium. The cells were cultured for 45 days under the conditions of culture temperature of 25±2℃, light intensity of 2000Lx and light time of 12h / d.

[0040] Culture results: The proliferation folds of adventitiou...

Embodiment 2

[0044] Example 2: Growth and rooting culture of adventitious shoots of Echinacea purpurea

[0045] (1) Obtainment of aseptic adventitious bud material of Echinacea purpurea: the same as step (1) of Example 1.

[0046] (2) Preparation of growth and rooting medium: On the basis of NECC1, NECC2, NECC3 and MS medium, sucrose 30 g / L, NAA 0.02 mg / L, and agar 4.8 g / L were added, and the pH value was adjusted to 6.0.

[0047] (3) Culture method: Inoculate the adventitious buds obtained in step (1) into NECC1, NECC2, NECC3 and MS medium prepared above. The cells were cultured for 45 days under the conditions of culture temperature of 25±2℃, light intensity of 2000Lx and light time of 12h / d.

[0048] (4) Transplanting method for rooted seedlings: unscrew the cap of the culture bottle and move it to natural light. After 3 days, carefully remove the plants from the medium, wash the medium on the roots, soak them in tap water for 12 hours, and plant them in a seedling tray containing moi...

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Abstract

The invention discloses a plant culture medium free of explosive compounds and an application thereof, belonging to the field of plant biotechnology. The plant culture medium without explosive compounds includes the following content of compound components that provide a large number of elements: 3500-4200 mg / L calcium ammonium nitrate, 1200-1600 mg / L potassium chloride, 370 mg / L heptahydrate sulfuric acid Magnesium and 170mg / L potassium dihydrogen phosphate. According to the needs of plants for ammonium nitrogen, 1000-1400mg / L ammonium chloride or 1000-1400mg / L ammonium sulfate can be added to supplement ammonium nitrogen. Compared with the MS medium, the medium of the present invention has little difference in composition, but the content of calcium ions is relatively high, which is conducive to promoting plant growth, has good tissue culture effect, and does not contain explosive compounds, and the composition is stable and safe. It is regulated and inexpensive, and has great application value to the tissue culture industry.

Description

technical field [0001] The invention belongs to the technical field of plant biology, and in particular relates to a plant culture medium that does not contain explosive compounds and its application. Background technique [0002] Plant culture medium is the material basis for the growth and development of in vitro materials in tissue culture, and is an essential production material for seedling production enterprises to apply biotechnology to produce seedlings. Usually plant tissue culture medium consists of inorganic nutrients, carbon sources, vitamins, retinoids and growth regulating substances. Inorganic nutrients include macroelements and trace elements; among them, macroelements refer to 6 elements that are in great demand, including nitrogen, phosphorus, potassium, calcium, magnesium and sulfur; trace elements refer to iron, boron, manganese, zinc, molybdenum, Cobalt and copper are 7 elements in low demand. [0003] To date, almost all medium formulations contain am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴鸿覃磊杨跃生宁熙平
Owner SOUTH CHINA AGRI UNIV
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