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Preparation and purification method of stevioside and application of stevioside in anti-fatigue products

A purification method and stevioside technology are applied in the preparation of sugar derivatives, chemical instruments and methods, medical preparations containing active ingredients, etc. Impurity removal effect and other issues, to achieve the effect of promoting flocculation and sedimentation, promoting the aggregation of impurities, and reducing the loss rate

Active Publication Date: 2021-06-11
BEIJING AOTE SHUER HEALTH PROD DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flocculant in this invention uses traditional non-modified chitosan and iron sulfate, and some components are also non-polymeric macromolecules, so it is very likely that the flocculation effect is poor and it is difficult to play key roles such as netting, which makes it difficult to To achieve a better impurity removal effect, it is difficult to meet the growing demand in the field of extraction and purification
[0004] Aiming at the problems of low impurity removal rate, high loss and low yield in the existing preparation and purification methods of steviol glycosides, a preparation and purification method with high impurity removal rate and low loss rate should be found to obtain high yield and high purity steviol glycosides

Method used

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  • Preparation and purification method of stevioside and application of stevioside in anti-fatigue products
  • Preparation and purification method of stevioside and application of stevioside in anti-fatigue products

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A method for preparing and purifying steviol glycosides, comprising the following steps:

[0036] (1) Extraction: after mixing stevia leaves with boiling water, carry out reflux extraction, the number of times of reflux extraction is 3 times, and each extraction time is 0.8h, to obtain extract A; wherein, the weight ratio of boiling water to stevia leaves dry leaves is 8 :1;

[0037] (2) Flocculation and precipitation: add flocculant to the extract A, heat and stir, stand still, centrifuge, and take the supernatant to get final product; wherein, the weight ratio of extract A to flocculant A is 80:1;

[0038] (3) Secondary extraction, refining, and concentration: use AB-8 macroporous adsorption resin to carry out secondary extraction on the supernatant obtained in step (2), pass through D101 type macroporous adsorption resin, and concentrate to obtain final product;

[0039] Among them, the flocculant includes: 20 parts of polyferric sulfate, 5 parts of activated carbon...

Embodiment 2

[0041] A method for preparing and purifying steviol glycosides, comprising the following steps:

[0042] (1) Extraction: after mixing stevia leaf with boiling water, carry out reflux extraction, the number of times of reflux extraction is 3 times, each extraction time is 1.2h, obtains extract A; Wherein, the weight ratio of boiling water and stevia leaf dry leaf is 12 :1;

[0043] (2) Flocculation and precipitation: add flocculant to extract A, heat and stir, stand still, centrifuge, take supernatant, and obtain it; wherein, the weight ratio of extract A to flocculant A is 90:1;

[0044] (3) Secondary extraction, refining, and concentration: use AB-8 macroporous adsorption resin to carry out secondary extraction on the supernatant obtained in step (2), pass through D101 type macroporous adsorption resin, and concentrate to obtain final product;

[0045]Among them, the flocculant includes: 30 parts of polyferric sulfate, 8 parts of activated carbon, 5 parts of ferrous sulfate,...

Embodiment 3

[0047] A method for preparing and purifying steviol glycosides, comprising the following steps:

[0048] (1) Extraction: Stevia leaves are mixed with boiling water to carry out reflux extraction, the number of times of reflux extraction is 3 times, and each extraction time is 1h to obtain extract A; wherein, the weight ratio of boiling water to stevia leaves dry leaves is 10: 1;

[0049] (2) Flocculation and precipitation: add a flocculant to the extract A, heat and stir, stand still, centrifuge, take the supernatant, and obtain it; wherein, the weight ratio of the extract A to the flocculant A is 100:1;

[0050] (3) Secondary extraction, refining, and concentration: use AB-8 macroporous adsorption resin to carry out secondary extraction on the supernatant obtained in step (2), pass through D101 type macroporous adsorption resin, and concentrate to obtain final product;

[0051] Among them, the flocculant includes: 25 parts of polyferric sulfate, 6 parts of activated carbon, ...

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Abstract

The invention provides a preparation and purification method of stevioside and application of the stevioside in an anti-fatigue product, and relates to the technical field of extraction. The preparation and purification method specifically comprises the following steps of (1), extraction: mixing stevia rebaudian and an extracting agent and then conducting reflux extraction to obtain an extracting solution A; (2) flocculating and precipitating: adding a flocculating agent into the extracting solution A, conducting filtering to remove precipitate to obtain filtrate; and (3) secondary extraction, refining and concentration: carrying out secondary extraction on the filtrate by using an adsorption column, conducting treatment through a refining column, and conducting concentrating, wherein the flocculating agent is prepared from polyferric sulfate, activated carbon, ferrous sulfate, ferric citrate, glycine iron, edible seaweed powder, starch and zeolite powder. By optimizing the components of the flocculating agent and the whole method, the impurity removal rate and the extraction rate can be effectively improved, the loss rate is reduced, and the product prepared by taking the stevioside obtained by the method as the raw material has an excellent anti-fatigue effect.

Description

technical field [0001] The invention relates to the technical field of extraction, in particular to a method for preparing and purifying steviol glycosides and its application in anti-fatigue products. Background technique [0002] Steviol glycoside is a glycoside extracted from the leaves of Stevia rebaudiana, a plant of Compositae, which is a tetracyclic diterpene monomer containing a variety of different components, including rebaudioside A, stevioside, rubusoside and And dulcoside A, etc., have high sweetness and no obvious toxic side effects. In addition, related studies have shown that steviol glycosides also have anti-inflammatory, anti-tumor, blood sugar and blood pressure inhibition, and dental caries prevention effects. [0003] Traditional preparation techniques of steviol glycosides include hot water extraction, dialysis, ultra-high pressure, supercritical CO 2 Extraction method and enzymatic method, etc., and the extraction and purification process usually need...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08A61K31/704A61K36/82A61P39/00
CPCA61K31/704A61K36/254A61K36/258A61K36/287A61K36/815A61K36/82A61P39/00C07H1/08C07H15/256A61K2300/00
Inventor 赵一弘雷雨
Owner BEIJING AOTE SHUER HEALTH PROD DEV
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