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Compound for treating colon cancer and application thereof

A compound and composition technology, applied in the field of medicine, can solve the problems of lower than expected curative effect of colon cancer, toxic and side effects of patients, etc., and achieve the effect of enhancing the anti-tumor effect

Pending Publication Date: 2021-06-15
SUN YAT SEN UNIV SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For many years, these conventional treatments have been less effective than expected for colon cancer and have produced many toxic side effects for many patients

Method used

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  • Compound for treating colon cancer and application thereof
  • Compound for treating colon cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 compound of the present invention

[0030] 1. Preparation of liposomes:

[0031] 1) Pre-cool the low-temperature coolant circulation pump to make the cooling temperature below 10°C;

[0032] 2) Take 2.5mgL-α-lysolecithin, 2.5mgL-α-phosphatidylethanolamine, 1mg cholesterol, 2mg cholesterol-peg600, add 4mL absolute ethanol to fully dissolve, transfer to a 25mL round bottom flask;

[0033] (For subsequent preparation of red fluorescent liposomes, 10 μL Atto 532 DOPE dye needs to be added in this step, stock solution concentration: 5 mg / mL)

[0034] 3) Turn on the rotating device, turn on the atmosphere, turn on the switch of the vacuum pump, and seal the joint with glue;

[0035] 4) Set the temperature of the water bath to 40°C, adjust the rotation speed to 150-180r / min, vacuumize and rotate for 1 hour, until the bottom of the flask is covered with a film and almost all the ethanol in the bottle is volatilized;

[0036] 5) Turn off the ro...

Embodiment 2

[0082] Embodiment 2 carries the characterization of black phosphorus quantum dot Adpgk polypeptide liposome

[0083] In order to check whether the prepared Adpgk polypeptide liposomes loaded with black phosphorus quantum dots meet the particle size requirements of drug-loaded nanosystems, their morphology was characterized under a scanning electron microscope, as shown in figure 2 (A represents the topography of the Adpgk polypeptide, B represents the topography of the Adpgk polypeptide liposome loaded with black phosphorus quantum dots, and C and D are the topography of the F127 gel with a concentration of 25%. ).

[0084] After freeze-drying, polypeptide antigens are fragmented and scattered under the scanning electron microscope, and after being encapsulated by liposomes, after freeze-drying, the spherical or ellipsoidal shape similar to liposomes is observed under the scanning electron microscope. And the particle size is shown at about 200nm, which is relatively close to...

Embodiment 3

[0085] Example 3 Cell experiment

[0086] 1. Cell uptake experiment

[0087] 1) Inoculate log phase HeLa or DC2.4 cells of appropriate density into 12-well plates (with cell slides in advance), at 37°C, 5% CO 2 Cultivate overnight in an incubator;

[0088] 2) When the density of adherent cells reaches 70-80% the next day, suck off the old medium;

[0089] 3) Add 750 μL of the culture medium required for the corresponding cells without fetal bovine serum, and then add 250 μL of the corresponding liposome sample to each well, and continue to incubate for 4 hours;

[0090] 4) Aspirate the old culture medium from each well, wash with 1mL 1×PBS buffer solution twice;

[0091] 5) Prepare a film, and observe the uptake effect of the corresponding cells on the liposomes loaded with black phosphorus quantum dots by confocal laser microscopy (CLSM).

[0092] 2. Cytotoxicity test - CCK 8 method

[0093] (1) Detection of cytotoxicity of liposomes loaded with black phosphorus quantum ...

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Abstract

The invention discloses a compound for treating colon cancer and application thereof. The compound comprises black phosphorus quantum dots, liposomes, Adpgk peptides and F127 gel; the Adpgk peptides and the black phosphorus quantum dots are wrapped by the liposomes to form Adpgk peptide black phosphorus quantum dot liposomes; the Adpgk peptide black phosphorus quantum dot liposomes are embedded in the F127 gel. According to the invention, the black phosphorus quantum dots with good photothermal effect and Adpgk polypeptides are effectively loaded by the liposomes, so that the combined application of a photothermal method and an immune method is realized; meanwhile, it is found that application of the black phosphorus quantum dots loaded Adpgk polypeptide liposome (Adpgk polypeptide / BPQDs / Liposome) has more advantages in the anti-tumor effect than application of the black phosphorus quantum dots loaded composite liposome, and it is indicated that the anti-tumor effect can be effectively enhanced through combined application of thermal therapy and immunological therapy.

Description

technical field [0001] The invention belongs to the field of medicine and relates to a compound for treating colon cancer and its application. Background technique [0002] Colon cancer is a disease caused by the interaction of genetic and environmental factors, which occurs in the colon and is a common malignant tumor of the gastrointestinal tract. Statistics show that in 2017, it is estimated that nearly 140,000 people in the United States will be diagnosed with colon cancer, and about 50,000 people will die from it; rate fifth. [0003] At present, the conventional treatment methods for colon cancer mainly include surgery, radiotherapy, chemotherapy, gene therapy and traditional Chinese medicine treatment. Chemotherapy is chemical drug treatment. Generally, after surgery, patients need to receive chemotherapy for 1 to 1.5 years, which is about 2 to 3 courses of treatment. During chemotherapy, patients may experience severe side effects, such as nausea, loss of appetite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/127A61K39/00A61K47/10A61P35/00
CPCA61K41/0052A61K9/127A61K39/0011A61K47/10A61P35/00A61K2039/82A61K2300/00
Inventor 梁欣张旭东梅林张錦勰
Owner SUN YAT SEN UNIV SHENZHEN
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