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Medicinal carbon dot modified probiotic preparation as well as preparation method and application thereof

A probiotic preparation and probiotic technology are applied in the field of medicine and can solve problems such as poor medication stability and poor biocompatibility, and achieve the effects of stable physical and chemical properties, simple preparation method and improved therapeutic effect.

Active Publication Date: 2022-07-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a carbon dot modified probiotic preparation and its preparation method and application, which can effectively solve the problems of poor drug stability and poor biocompatibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of a medicinal carbon dot modified probiotic preparation of the present invention is:

[0019] (1) Preparation of drug carbon dots: add 1.5 g of model drug quercetin into 10 mL of ultrapure water, hydrothermally react at 120 °C for 15 h, filter with filter paper with a pore size of 100 nm, centrifuge at 10000 rpm for 8 min, and dialyze overnight with a 2000 Da dialysis bag for 9 h , freeze-dried to obtain quercetin carbon dots;

[0020] (2) Preparation of medicinal carbon dot modified probiotics preparation: the number of viable bacteria is 2 × 10 9 1 g of CFU bifidobacteria was added to 1 mL of quercetin carbon dots with a mass concentration of 40 mg / mL in 0.9% normal saline solution, stirred at room temperature for 9 hours, centrifuged at 5000 rpm for 10 min, and washed with 0.9% normal saline (NaCl) with a mass concentration of 0.9%. 3 times, each dose of 1 mL, to obtain a quercetin carbon dot modified probiotic preparation.

Embodiment 2

[0022] The preparation method of a medicinal carbon dot modified probiotic preparation of the present invention is:

[0023] (1) Preparation of drug carbon dots: Add 0.6 g of model drug resveratrol to 8 mL of ultrapure water, hydrothermally react at 115 °C for 23 h, filter with filter paper with a pore size of 100 nm, centrifuge at 8500 rpm for 9 min, and dialyze overnight with a 600 Da dialysis bag 10h, freeze-drying to obtain resveratrol carbon dots;

[0024] (2) Preparation of medicinal carbon dot modified probiotics preparation: the number of viable bacteria is 3×10 9 CFU Escherichia coli Nissle 19171g was added to 1 mL of a 0.9% physiological saline solution with a mass concentration of 40 mg / mL resveratrol carbon dots, stirred at room temperature for 7 hours, centrifuged at 5000 rpm for 10 min, and then mixed with 0.9% physiological saline (NaCl) with a mass concentration of 0.9%. ) washed 3 times, each dose of 1 mL, to obtain a resveratrol carbon dot modified probiotic...

Embodiment 3

[0026] The preparation method of a medicinal carbon dot modified probiotic preparation of the present invention is:

[0027] (1) Preparation of drug carbon dots: add 1.0 g of the model drug curcumin to 9 mL of ultrapure water, hydrothermally react at 125 °C for 7 h, filter with filter paper with a pore size of 100 nm, centrifuge at 9000 rpm for 8 min, and dialyze overnight with a 1000 Da dialysis bag for 9 h. Freeze-drying to obtain curcumin carbon dots;

[0028] (2) Preparation of medicinal carbon dot modified probiotics preparation: count 1×10 viable bacteria 5 1 g of Lactobacillus CFU was added to 1 mL of a 0.9% normal saline solution with a mass concentration of 40 mg / mL curcumin carbon dots, stirred at room temperature for 8 hours, centrifuged at 5000 rpm for 10 min, and washed three times with 0.9% normal saline (NaCl) with a mass concentration of 0.9%. The dosage of each time is 1 mL to obtain a curcumin carbon dot modified probiotic preparation.

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Abstract

The invention relates to a carbon dot modified probiotic preparation and a preparation method and application thereof, which can effectively solve the problems of poor medication stability and poor biocompatibility, and the method comprises the following steps: adding a model drug into ultrapure water, carrying out hydrothermal reaction, filtering with filter paper, centrifuging, carrying out overnight dialysis with a dialysis bag, and freeze-drying to obtain drug carbon dots; adding probiotics into the medicine carbon dot normal saline solution, stirring at room temperature, centrifuging, and washing with normal saline for 3 times to obtain the medicine carbon dot modified probiotic preparation. The preparation method is simple, the prepared product is stable in physicochemical property, the medicine is delivered to the whole body, the treatment effect is greatly improved, more colonization of probiotics in the intestinal tract is protected, intestinal flora disorder is improved, combined application is achieved, the preparation method is a great innovation for treating the diseases, and huge social and economic benefits are achieved.

Description

technical field [0001] The invention relates to medicine, in particular to a medicinal carbon point modified probiotic preparation and a preparation method and application thereof. Background technique [0002] Oral administration is the most commonly used route of administration, especially for chronic diseases, and compared with intravenous administration, oral administration allows patients to self-administer, reducing health care costs and improving patient compliance. In the past few decades, natural bioactive organic compounds such as resveratrol, curcumin, and quercetin have been implicated in chronic diseases such as insulin resistance, type 2 diabetes, and intestinal inflammatory diseases such as ulcerative colitis. As well as the powerful preventive and therapeutic effects of cardiovascular disease, it has attracted widespread attention. However, these lipid-soluble drugs all have the disadvantages of unstable physicochemical properties, poor water solubility, sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K35/741A61K35/745A61K47/04A61P5/50A61P1/00A61P29/00A61P9/00A61P3/10A61K31/05A61K31/12A61K31/352A61K31/56A61K31/09A61K31/355
CPCA61K35/747A61K35/741A61K35/745A61K31/05A61K31/12A61K31/352A61K31/56A61K31/09A61K31/355A61K47/02A61P5/50A61P1/00A61P29/00A61P9/00A61P3/10A61K2300/00Y02A50/30
Inventor 侯琳张振中李宏张依然许子强卢思宇王柏扬朱玲张红岭
Owner ZHENGZHOU UNIV
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