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A kind of low cleaning residue magnetic beads and preparation method thereof

A magnetic bead and reaction technology, which is applied in the preparation methods of peptides, the preparation of carboxylate esters, chemical instruments and methods, etc., can solve the problems of difficult cleaning and other problems, and achieve the effects of low cleaning residues, reducing residues, and easy washing away.

Active Publication Date: 2022-06-24
JIANGSU JITRI MOLECULAR ENG INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this process, electromagnetic interaction will occur between the electrode surface and the magnetic bead surface, so that the magnetic beads are adsorbed on the electrode surface, making it difficult to clean the electrode surface after detection

Method used

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  • A kind of low cleaning residue magnetic beads and preparation method thereof
  • A kind of low cleaning residue magnetic beads and preparation method thereof
  • A kind of low cleaning residue magnetic beads and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0034] At the same time, the present invention provides a preparation method of low-cleaning residual magnetic beads, which comprises the preparation steps:

[0035] 1) Preparation of activated ester mixture

[0036] Put 5-(4,4'-dimethoxytriphenylmethoxy)-pentanoic acid and 4-pentynoic acid in a reaction tube, add dicyclohexylcarbodiimide and N-hydroxysuccinyl imine and DMF react at room temperature to obtain a mixture of activated esters, wherein the 5-(4,4'-dimethoxytriphenylmethoxy)-pentanoic acid, 4-pentynoic acid, di The mass volume ratio of cyclohexylcarbodiimide, N-hydroxysuccinimide and DMF is 50mg:25mg:25mg:13mg:1mL

[0037] 2) The surface of the magnetic beads is modified with hydroxyl and alkynyl groups

[0038] Add the activated ester mixture prepared in step 1) to the amino magnetic beads, shake the reaction at room temperature, and then add a DMF solution with a mass percentage of 10% TFA to obtain the magnetic beads with hydroxyl and alkynyl surface modificati...

Embodiment 1

[0048]19.3 g of benzyl 5-hydroxyvalerate was added to the reaction flask under argon protection, 100 mL of pyridine was added to the reaction flask, and the solid was completely dissolved by stirring; 37.7 g of 4,4'-dimethoxytris Phenylchloromethane (DMT-Cl), 0.2 g 4-dimethylaminopyridine (DMAP), keep the reaction at room temperature for 16 h, TLC monitoring the raw material reaction is complete; 10 mL methanol is added to quench the reaction, and the solvent is removed under reduced pressure at 40 °C; Add 150 mL of ethyl acetate and 100 mL of saturated aqueous sodium bicarbonate solution to separate the organic phase; wash the organic phase with saturated brine; add anhydrous sodium sulfate to dry the organic phase; through flash column chromatography PE:EA= After 30:1-20:1 separation, 13.0 g of product was obtained.

[0049] The structural formula of this product is:

[0050] .

[0051] 13.0 g of the product obtained in the previous step, 200 mL of ethyl acetate and 6 mL...

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Abstract

The invention belongs to the technical field of electrochemical detection materials, and in particular relates to a magnetic bead with low cleaning residue and a preparation method thereof. The magnetic beads are surface-modified magnetic beads with hydroxyl and alkyne groups and tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine-copper(I) complex Prepared by reacting with azide-labeled streptavidin. The beneficial effect of the present invention is: the present invention carries out the modification of hydroxyl and alkynyl to the surface of amino magnetic beads first; Amino-copper (I) complexes and azide-labeled streptavidin were used to further modify the surface of amino magnetic beads to obtain a low cleaning residue magnetic beads, which effectively reduced the magnetic beads on the electrode surface. Residue can be more easily washed off by cleaning solution.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection materials, and particularly relates to a low-cleaning residual magnetic bead and a preparation method thereof. Background technique [0002] Magnetic beads are a new immunological technology developed, which combines the unique advantages of immobilized reagents with the high specificity of immunological reactions. Based on immunology, it penetrates into pathological, physiological, pharmacological, microbiological, biochemical and In various fields such as molecular genetics, it has been more and more widely used in immune detection, cell separation, biological macromolecular purification and molecular biology. [0003] In electrochemiluminescence detection using magnetic beads, a redox reaction occurs on the electrode surface after the magnetic beads are energized. In this process, electromagnetic interaction occurs between the surface of the electrode and the surface of the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/36C07K1/13C07K1/113C07C51/09C07C59/68C07C67/31C07C69/712
CPCC07K14/36C07C51/09C07C67/31C07C69/712C07C59/68
Inventor 杨志伟刘铮胡春苗
Owner JIANGSU JITRI MOLECULAR ENG INST CO LTD