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A compound probiotic fermented product with skin care efficacy and its preparation and application

A technology of probiotics and fermented liquid, applied in skin care preparations, medical preparations containing active ingredients, fermentation, etc., can solve the problem of lack of active metabolite skin care function, lack of stable fermentation process, high active metabolite rich Integrate technology and other issues to achieve the effect of maintaining the balance of flora, repairing the skin barrier, and improving skin care efficacy

Active Publication Date: 2022-07-05
青岛元达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the prior art lacks research on the stable fermentation process of probiotic metabolites, the enrichment process of highly active metabolites, and the skin care function; specifically, (1) the lack of fermentation media optimized for skin care functions with high yields of probiotic active metabolites , time, temperature and other fermentation technologies, thereby improving the yield and stability of beneficial metabolites; (2) lack of fermentation technology using compound lactic acid bacteria fermentation technology to produce highly active and rich types of beneficial substances; (3) lack of active metabolism Research on skin care function

Method used

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  • A compound probiotic fermented product with skin care efficacy and its preparation and application
  • A compound probiotic fermented product with skin care efficacy and its preparation and application
  • A compound probiotic fermented product with skin care efficacy and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: A postbiotic Bilantera with skin care function and its preparation

[0032] (1) Lactobacillus plantarum CGMCC No.11932, Lactobacillus casei CGMCC No.18638, Bifidobacterium lactis CGMCC No.14536, Bifidobacterium animalis CGMCC No.16070 and Lactobacillus rhamnosus CGMCC No.16002 strains were respectively It was inoculated into MRS liquid medium, incubated at 37°C for 18 hours, and subcultured twice, so that the number of viable bacteria after activation of each strain was 5-20 billion cfu / ml.

[0033] (2) The activated Lactobacillus plantarum CGMCC No.11932, Lactobacillus casei CGMCC No.18638, Bifidobacterium lactis CGMCC No.14536, Bifidobacterium animalis CGMCC No.16070 and Lactobacillus rhamnosus CGMCC No.16002 The bacterial species are mixed into a probiotic composition in a ratio of 1: 1: 2: 2: 0.5 in terms of the number of viable bacteria.

[0034](3) The above probiotic composition is inoculated into the fermentation medium according to the inoculation a...

Embodiment 2

[0039] Example 2: Research on skin care function of postbiotic Bilantera

[0040] Through the clinical efficacy test of Postbiotic Bilantera, the changes of various skin indicators before and after application were studied to verify its effect on wrinkle removal and skin barrier repair. The cited standards are: "Guidelines for the Evaluation of Moisturizing Efficacy of Cosmetics" QB / T4256-2011, "Test Methods for Transepidermal Moisture Loss of Cosmetic Effects" T / ZHCA003-2018 and internal testing standards of institutions.

[0041] The test method is as follows:

[0042] (1) Test sample: Postbiotic Bilantera prepared in Example 1;

[0043] Control sample: thickener 1%, preservative 0.5%, humectant 3%, stabilizer 2%, and the rest is deionized water.

[0044] (2) Test equipment

[0045] Facial image analysis VISIA image analysis software;

[0046] Skin elasticity test probe Cutometer MPA580 (Courage & Khazaka, Germany);

[0047] Skin melanin test probe MexameterMX18 (Courag...

Embodiment 3

[0083] Example 3: Content of active substances in probiotic fermentation broth

[0084] Taking the postbiotic fermentation broth prepared in Example 1 as the test sample, the active substances and their contents were analyzed based on the UPLC-Q-TRAP-MS targeted metabolic analysis method, as follows:

[0085] (1) Sample pretreatment

[0086] Take 0.1 g of the sample into a 2 mL homogenization tube filled with 1.0 mm zirconia beads, add 1 mL of ultrapure water and mix well, use OMNI Bead Ruptor 12 (OMNI International, USA) to homogenize the mixture at 3 m / s Incubate for 30 s, repeat twice; transfer all to a 7 mL centrifuge tube, add 30 μL of 50 μg / mL bile acid mixed IS solution and 2 mL of acetonitrile, mix well, and centrifuge at 13,400 × g for 10 min. Take the supernatant and pass it through a 0.22 μm filter, transfer 50 μL to a 2 mL liquid sample bottle containing 450 μL methanol, mix well and use the machine for detection. (2) UPLC-Q-TRAP-MS detection conditions

[0087]...

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Abstract

The invention belongs to the field of microbial fermentation, and particularly relates to a fermentation metabolite with skin care function and a preparation method and application thereof. The product is prepared by mixing Lactobacillus plantarum, Lactobacillus casei, Bifidobacterium lactis, Bifidobacterium animalis and Lactobacillus rhamnosus as fermentation strains, and sucrose, seaweed powder, soybean powder and milk powder as fermentation raw materials. The product contains malonyl genistein, genistein, hyaluronic acid, iridone, Preaustinoid A and daidzein and other unique fermentation metabolites to help improve skin care efficacy; fermented tartaric acid, malic acid, succinic acid Organic acids such as salicylic acid, vanillic acid and phenylpropionic acid can control pathogenic bacteria and spoilage microbial flora, help maintain the balance of skin flora, and play a natural antiseptic role. Through the efficacy test, it has been shown that it has the functions of wrinkle reduction, increased skin elasticity, anti-inflammatory and skin barrier repair, and the effect is remarkable.

Description

Technical field: [0001] The invention relates to a probiotic fermentation product, in particular to a fermentation metabolite with skin care function, a preparation method and application thereof, and belongs to the field of microbial fermentation. Background technique: [0002] Lactic acid bacteria and their fermented products play an important role in ensuring the healthy life of human beings, and are a civilian production industry that carries human nutrition and health. A large number of studies have shown that the probiotic effect of probiotic lactic acid bacteria is related to its activity, especially the metabolites of probiotic bacteria, the lysate of the cell body and the cell body itself may be the direct promoters of the health effect. . Beneficial metabolites produced by lactic acid bacteria, such as exopolysaccharides, cell wall peptides, short-chain fatty acids, lactobacilli, antibacterial organic acids, etc., are used in regulating immunity, anti-tumor, antib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A61K8/99A61Q17/00A61Q19/00A61Q19/08C12R1/25C12R1/245C12R1/225C12R1/01
CPCC12P1/04C12N1/20A61K8/99A61Q19/00A61Q19/08A61Q17/005A61K2800/5922A61K2800/85A61K2800/524Y02A40/90
Inventor 彭传涛王习振
Owner 青岛元达生物科技有限公司
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